1887

Abstract

Summary: The presence of an incomplete tricarboxylic acid cycle in and is described. These blue-green algae lack both -oxoglutarate dehydrogenase and succinyl-CoA synthetase. Succinyl-CoA was formed in extracts of by 3-ketoacyl CoA-transferase using acetoacetyl-CoA as CoA donor. The activities of the other tricarboxylic cycle enzymes were measured in extracts prepared from autotrophic organisms and from those grown in the presence of acetate. No alterations in activity indicative of enzyme repression or de-repression were observed. These results are discussed in relation to possible reasons for autotrophic behaviour.

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1969-03-01
2024-04-18
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References

  1. Bulen W. A. 1956; The isolation and characterization of glutamic dehydrogenase from com leaves. Archs Biochem. Biophys 62:173
    [Google Scholar]
  2. Callely A. G., Rigopoulos N., Fuller R. C. 1968; The assimilation of carbon by Chloropseudomonas ethylicum . Biochem. J 106:615
    [Google Scholar]
  3. Carr N. G. 1967; Aspects of enzymic control in blue-green algae. Abstr. 5th Meeting Fedn. Europ. Biochem. Soc Universitetsforlaget, Oslo: p. 89
    [Google Scholar]
  4. Carr N. G., Hallaway M. H. 1965; Reduction of phenolindo-2,6-dichlorophenol in dark and light by the blue-green alga, Anabaena variabilis . J. gen. Microbiol 39:335
    [Google Scholar]
  5. Carr N. G., Lascelles J. 1961; Some enzymic reactions concerned in the metabolism of aceto-acetyl-coenzyme A in Athiorhodaceae. Biochem. J 80:70
    [Google Scholar]
  6. Carr N. G., Pearce J. 1966; Photoheterotrophism in blue-green algae. Biochem. J 99:28P
    [Google Scholar]
  7. Ells H. A. 1959; A colorimetric method for the assay of soluble succinic dehydrogenase and pyridine nucleotide-linked dehydrogenase. Archs Biochem. Biophys 85:561
    [Google Scholar]
  8. Forget P., Pichinoty F. 1967; Le cycle tricarboxylique chez Aerobacter aerogenes . Annls Inst. Pasteur, Paris 112:261
    [Google Scholar]
  9. Fuller R. C., Smillie R. M., Sisler E. C., Kornberg H. L. 1961; Carbon metabolism in Chromatium. J. biol. Chem 236:2140
    [Google Scholar]
  10. Hathaway J. A., Atkinson D. E. 1963; The effect of adenylic acid on yeast nicotinamide adenine dinucleotide isocitrate dehydrogenase, a possible metabolic control mechanism. J. biol. Chem 238:2875
    [Google Scholar]
  11. Hempfling W. P., Vishniac W. 1965; Oxidative phosphorylation in extracts of Thiobacillus. Biochem. Z 342:272
    [Google Scholar]
  12. Hoare D. S., Moore R. B. 1965; Photoassimilation of organic compounds by autotrophic blue-green algae. Biochim. Biophys. Acta 109:622
    [Google Scholar]
  13. Hoare D. S., Hoare S. L., Moore R. B. 1967; The photoassimilation of organic compounds by autotrophic blue-green algae. J. gen. Microbiol 49:351
    [Google Scholar]
  14. Hood W., Carr N. G. 1968; Threonine deaminase and acetolactate synthetase in Anabaena variabilis . Biochem. J 10:1094P
    [Google Scholar]
  15. Kaufman S., Gilvarg C., Cori O., Ochoa S. 1953; Enzymatic oxidation of α-ketoglutarate and coupled phosphorylation. J. biol. Chem 203:869
    [Google Scholar]
  16. Kelly D. P. 1967; The incorporation of acetate by the chemoautotroph Thiobacillus neapolitanus strain c. Arch. Mikrobiol 58:99
    [Google Scholar]
  17. Kornberg A., Pricer W. E. 1951; Di- and triphosphopyridine nucleotide isocitric dehydrogenase in yeast. J. biol. Chem 189:123
    [Google Scholar]
  18. Leach C. K., Carr N. G. 1968; Reduced nicotinamide adenine dinucleotide phosphate oxidase in the autotrophic blue-green alga, Anabaena variabilis . Biochem. J 109:4P
    [Google Scholar]
  19. Lynen F., Henning U., Bublitz C., Sorbo B., Kröplin-Rueff L. 1958; Der chemische Mechanismus der Acetessigsäurebildung in der Leber. Biochem. Z 330:269
    [Google Scholar]
  20. Mehler A. H., Kornberg A., Grisolia S., Ochoa S. 1948; The enzymatic mechanism of oxidation reductions between malate or isocitrate and pyruvate. J. biol. Chem 174:961
    [Google Scholar]
  21. Ochoa S. 1948; Biosynthesis of tricarboxylic acids by carbon dioxide fixation. J. biol. Chem 174:133
    [Google Scholar]
  22. Ochoa S. 1955; Crystalline condensing enzyme from pig heart. Meth. Enzymol 1:685
    [Google Scholar]
  23. Ochoa S., Mehler A. H., Kornberg A. 1948; Biosynthesis of dicarboxylic acids by carbon dioxide fixation. J. biol. Chem 174:979
    [Google Scholar]
  24. Pearce J., Carr N. G. 1967a; The metabolism of acetate by the blue-green algae, Anabaena variabilis and Anacystis nidulans . J. gen. Microbiol 49:301
    [Google Scholar]
  25. Pearce J., Carr N. G. 1967b; An incomplete tricarboxylic acid cycle in the blue-green alga, Anabaena variabilis . Biochem. J 105:45P
    [Google Scholar]
  26. Pearce J., Carr N. G. 1968; The incorporation and metabolism of glucose by Anabaena variabilis . J. gen. Microbiol 54:451
    [Google Scholar]
  27. Racker E. 1950; Spectrophotometric measurement of the enzymatic formation of fumaric and cis-aconitic acids. Biochim. biophys. Acta 4:211
    [Google Scholar]
  28. Simon E. J., Shemin D. 1953; The preparation of S-succinyl coenzyme A. J. Am. chem. Soc 75:2520
    [Google Scholar]
  29. Smith A. J., London J., Stanier R. Y. 1967; Biochemical basis of obligate autotrophy in blue-green algae and thiobacilli. J. Bact 94:972
    [Google Scholar]
  30. Stern J. R., Coon M. J., del Campillo A. 1953; Acetoacetyl coenzyme A as intermediate in the enzymatic breakdown and synthesis of acetoacetate. J. Am. chem. Soc 75:1517
    [Google Scholar]
  31. Trudinger P. A., Kelly D. P. 1968; Reduced nicotinamide adenine dinucleotide oxidation by Thiobacillus neopolitanus and Thiobacillus strain c. J. Bact 95:1962
    [Google Scholar]
  32. Trüper H. G. 1965; Tricarboxylic acid cycle and related enzymes in Hydrogenomonas strain H 16 G+ grown on various carbon sources. Biochim. biophys. Acta 111:565
    [Google Scholar]
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