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Volume 41, Issue 4

Detection by polymerase chain reaction of genes encoding aminoglycoside-modifying enzymes in methicillin-resistant isolates of epidemic phage types Free

Abstract

Summary

The polymerase chain reaction (PCR) was used to identify the and genes, encoding the aminoglycoside-modifying enzymes AAC(6′)-APH(2″), APH(3′)III and ANT(4′ 4″), respectively, and the methicillin resistance determinant in epidemic aminoglycoside and methicillin-resistant isolates of In total, 37 isolates collected in the period 1980–1985 and 81 isolates from the period 1991–1992 were obtained from 10 different Belgian hospitals. Epidemic isolates from the earlier period were characterised by phage type C (6/47/54/75) of phage group III, whereas two other epidemic phage types of group III—types A (77) and B (47/54/75/77/84/85)—were commonest in isolates from the second period. The bifunctional AAC(6′)-APH(2) was the enzyme encountered most frequently. The prevalence of APH(3 )III decreased significantly in the 1991–1992 period, while ANT(4′, 4″) was found solely in isolates from this period. Resistance mechanisms were more complex in isolates from the 1991–1992 period and the gene was detected in all isolates. The PCR results corresponded well with those obtained in the radiochemical phosphocellulose paper binding assay. Isolates from the 1991–1992 period were shown to express significantly higher levels of acetyltransferase activity than isolates from the 1980s.

  • Received:
  • Accepted:
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© 1994 The Pathological Society of Great Britain and Ireland
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1994-10-01
2024-04-16
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Detection by polymerase chain reaction of genes encoding aminoglycoside-modifying enzymes in methicillin-resistant Staphylococcus aureus isolates of epidemic phage types
J. Med. Microbiol. 41, 282 (1994); https://doi.org/10.1099/00222615-41-4-282
/content/journal/jmm/10.1099/00222615-41-4-282
/content/journal/jmm/10.1099/00222615-41-4-282
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