1887

Abstract

Summary: ATCC 12475 fails to produce streptomycin when grown at 34 °C or above, although growth is appreciable up to at least 37 °C. This depression of streptomycin production at elevated growth temperature is manifest equally in liquid and on solid, and with complex and minimal, media. We report studies with gene fusions of the reporter genes or to restriction fragments containing the streptomycin biosynthesis promoter . constructs were in high, and constructs in low, copy number vectors. Two promoter fragments were used, one requiring activation by the pathway-specific activator of , the other reportedly activator independent. expression in the constructs in and in was significantly reduced at 37 °C compared to 30 °C. Some of this reduction could be explained by lower plasmid copy number at the higher temperature, but -dependent expression was clearly temperature controlled. Using the reporter system, the temperature dependence of expression was confirmed but, surprisingly, the dependence of the two promoter fragments differed from that observed in the multicopy constructs. These data identify a temperature-dependent promoter which may contribute to the depressive effect of elevated growth temperature on streptomycin production.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-141-10-2511
1995-10-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/10/mic-141-10-2511.html?itemId=/content/journal/micro/10.1099/13500872-141-10-2511&mimeType=html&fmt=ahah

References

  1. Clayton T.M., Bibb M.J. 1990; Streptoniyces promoter-probe plasmids that utilize the xylEgene of Pseud aromas putida.. Nucleic Reids Rei 18:1077
    [Google Scholar]
  2. Distler J., Braun C., Ebert A., Piepersberg W. 1987a; Gene cluster for streptomycin biosynthesis inStreptomyces griseus: analysis of a central region including the major resistance gene.. Mol & Gen Genet 208:204–210
    [Google Scholar]
  3. Distler J., Ebert A., Mansouri K., Pissowotzki K., Stockmann M., Piepersberg W. 1987b; Gene cluster for streptomycin bio- synthesis in Streptomyces griseus: nucleotide sequence of three genes and analysis of transcriptional activity.. Nucleic Acids Res 15:8041–8056
    [Google Scholar]
  4. Distler J., Mansouri K., Mayer G., Stockmann M., Piepersberg W. 1992; Streptomycin production and its regulation.. Gene 115:105–111
    [Google Scholar]
  5. Fazeli M.R., Cove J.H., Baumberg S. 1995; Physiological factors affecting streptomycin production byStreptomyces griseusATCC 12475 in batch and continuous culture.. FEMS Microbiol Lett 126:55–62
    [Google Scholar]
  6. Hara O., Beppu T. 1982; Mutants blocked in streptomycin production in Streptoniycesgriseus - the role of A-factor.. J Antibiot 35:349–358
    [Google Scholar]
  7. Hopwood D.A., Kieser T., Wright H.M., Bibb M.J. 1983; Plasmids, recombination and chromosome mapping in Streptomyces lividans 66.. J Gen Microbiol 129:2257–2269
    [Google Scholar]
  8. Hopwood D.A., Bibb M.J., Chater K.F., Kieser T., Bruton C.J., Kieser H.M., Lydiate D.J., Smith C.P., Ward J.M., Schrempf H. 1985 Genetic Manipulation of Streptomyces: a Laboratory Manual. Norwich:: John Innes Foundation;
    [Google Scholar]
  9. Ingram C., Brawner M., Youngman P., Westpheling J. 1989; xylE functions as an efficient reporter gene in Streptomyces spp. : use for the study of galP1, a catabolite-controlled promoter.. J Bacterial 171:6617–6624
    [Google Scholar]
  10. Kell D.B., van Dam K., Westerhoff H.V. 1989; Contro1 analysis of microbial growth and productivity.. Symp soc Gen Microbiol 44:61–93
    [Google Scholar]
  11. Lindley H.K., Deeble V.J., Peschke U., O´Neill M., Baumberg S., Cove J.H. 1995; Dependence on reporter gene of apparent activity in gene fusions of a Streptomyces griseusstreptomycin biosynthesis promoter.. Can J Microbiol 41:407–417
    [Google Scholar]
  12. Martín J.-F., Demain A.L. 1980; Control of antibiotic biosynthesis.. Microbiol Rev 44:230–251
    [Google Scholar]
  13. Miyake K., Kuzuyama T., Horinouchi S., Beppu T. 1990; The A-factor-binding protein of Streptomyces griseusnegatively controls streptomycin production and sporulation.. J Bacteriol 172:3003–3008
    [Google Scholar]
  14. O´Neill M. 1992 Genetic studies on the streptomycin biosynthesis system of Streptoniyces griseus and its control. PhD thesis,University of Leeds
    [Google Scholar]
  15. Parker R.E. 1973 Introductory Statistics for Biology. London: Edward Arnold;
    [Google Scholar]
  16. Piepersberg W. 1994; Streptomycin and related aminoglycoside antibiotics.. In Biochemistry and Genetics of Antibiotic Biosynthesis pp. 71–104 Edited by Vining L., Stuttard C. Stoneham, MA: Butterworth-Heinemann;
    [Google Scholar]
  17. Retzlaff L., Mayer G., Beyer S., Ahlert J., Verseck S., Distler J., Piepenberg W. 1993; Streptomycin production instreptomycetes: a progress report.. In Industrial Microorganisms: Basic and Applied Molecular Genetics pp. 183–194 Edited by Baltz R. H., Hegeman G. D., Skatrud P.L. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  18. Vallins W.J.S., Baumberg S. 1985; Cloning of a DNA fragment from Xtreptoniycesgriseuswhich directs streptomycin phosphotransferase activity.. J Gen Microbiol 131:1657–1669
    [Google Scholar]
  19. Votruba J., Vanek Z. 1989; Physicochemical factors affecting actinomycete growth and secondary metabolism.. In Regulation of Secondary Metabolism in Actinomycetes pp. 264–282 Edited by Shapiro S. Boca Raton, FL: CRC Press;
    [Google Scholar]
  20. Vujaklija D., Ueda K., Hong S.-K., Beppu T., Horinouchi S. 1991; Identification of an A-factor-dependent promoter in the streptomycin biosynthetic gene cluster of Streptomyces griseus.. Mol & Gen Genet 229:119–128
    [Google Scholar]
  21. Vujaklija O., Horinouchi S., Beppu T. 1993; Detection of an A-factor-responsive protein that binds to the upstream activation sequence of i/rR, a regulatory gene for streptomycin biosynthesis in Streptoniyces griseus.. J Bacteriol 175:2652–2661
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-141-10-2511
Loading
/content/journal/micro/10.1099/13500872-141-10-2511
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error