1887

Abstract

Using different 5-fluoropyrimidine analogues, positive selection procedures for obtaining mutants blocked in pyrimidine and purine salvage genes of were established. Strains lacking the following enzyme activities due to mutations in the corresponding genes were isolated: uracil phosphoribosyltransferase uridine/cytidine kinase pyrimidine nucleoside phosphorylase , cytidine/deoxycytidine deaminase , thymidine kinase and purine nucleoside phosphorylase Based on an analysis of the mutants obtained, the pathways by which metabolizes uracil and the different pyrimidine nucleosides were verified. The substrate specificities of the different enzymes were determined. It was demonstrated that a single pyrimidine nucleoside phosphorylase accounts for the phosphorolytical cleavage of uridine, deoxyuridine and thymidine, and a single purine nucleoside phosphorylase has activity towards both the ribonucleoside and deoxyribonucleoside derivatives of adenine, guanine and hypoxanthine. No phosphorylase activity towards xanthosine appeared to be present. The selection procedures developed during this work may be employed in establishing markers on the chromosome of many related lactic acid bacteria.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-141-8-1883
1995-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/8/mic-141-8-1883.html?itemId=/content/journal/micro/10.1099/13500872-141-8-1883&mimeType=html&fmt=ahah

References

  1. Ahmad S.I., Pritchard R.H. 1969; A map of four genes specifying enzymes involved in catabolism of nucleosides and deoxynucleosides in Escherichia coli. . Mol & Gen Genet 104:351–359
    [Google Scholar]
  2. Beck C.F., Ingraham J.L. 1971; Location on the chromosome of Salmonella typhimurium of genes governing pyrimidine metabolism. Mol & Gen Genet 111:303–316
    [Google Scholar]
  3. Beck C.F., Ingraham J.L., Neuhard J. 1972; Location on the chromosome of Salmonella typhimurium of genes governing pyrimidine metabolism. II. Uridine kinase, cytosine deaminase, and thymidine kinase. Mol & Gen Genet 115:208–215
    [Google Scholar]
  4. Gasson M.J. 1983; Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast induced curing. J Bacteriol 154:1–9
    [Google Scholar]
  5. Hammer-Jespersen K., Buxton R.S., Hansen T.D. 1980; A second purine nucleoside phosphorylase in Escherichia coli K-12. Mol & Gen Genet 179:341–348
    [Google Scholar]
  6. Heidelberger C., Danenberg P.V., Moran R.G. 1983; Fluorinated pyrimidines and their nucleosides. In Advances in Entomology and Belated Areas in Molecular Biology pp. 57–119 Meister A. Edited by New York:: John Wiley.;
    [Google Scholar]
  7. Jensen K.F. 1978; Two purine nucleoside phosphorylases in Bacillus subtilis. Purification and some properties of the adenosine- specific phosphorylase. Biochim Biophys Acta 525:346–356
    [Google Scholar]
  8. Jensen K.F., Nygaard P. 1975; Purine nucleoside phosphorylase from Escherichia coli and Salmonella typhimurium. . Eur J Biochem 51:253–265
    [Google Scholar]
  9. Jensen P.R., Hammer K. 1993; Minimal requirements for exponential growth of Lactococcus lactis. . Appl Environ Microbiol 59:4363–4366
    [Google Scholar]
  10. Kunji E.R., Smid E.J., Plapp R., Poolman B., Konings W.N. 1993; Di-tripeptides and oligopeptides are taken up via distinct transport mechanisms in Lactococcus lactis. . J Bacteriol 175:2052–2059
    [Google Scholar]
  11. Lautier M., Gillot B., Auffray Y., Castillo-Sanches X., Boutibonnes P. 1988; Mutagenesis in Streptococcus lactis exposed to UV irradiation and alkylating agents. Mutagenesis 3:245–247
    [Google Scholar]
  12. Leer J.C, Hammer-Jesperson K., Schwartz M. 1977; Uridine phosphorylase from Escherichia coli. Physical and chemical characterization. Eur J Biochem 77:217–224
    [Google Scholar]
  13. Martinussen J., Hammer K. 1994; Cloning and sequencing of upp; a gene encoding uracil phosphoribosyltransferase from Lactococcus lactis. . J Bacteriol 176:6457–6463
    [Google Scholar]
  14. Martinussen J., Andersen P.S., Hammer K. 1994; Nucleotide metabolism in Lactococcus lactis: salvage pathways of exogenous pyrimidines. J Bacteriol 176:1514–1516
    [Google Scholar]
  15. Martinussen J., Glasér P., Andersen P.S., Saxild H.H. 1995; Two genes encoding uracil phosphoribosyltransferase present in Bacillus subtilis. . J Bacteriol 177:271–274
    [Google Scholar]
  16. Neuhard J. 1983; Utilization of preformed pyrimidine bases and nucleosides. InMetabolism of Nucleotides, Nucleosides and Nucleobases in Microorganisms pp. 95–148 Munch-Petersen A. Edited by London:: Academic Press.;
    [Google Scholar]
  17. Nilsson D., Lauridsen A.A. 1992; Isolation of purine auxotrophic mutants of Lactococcus lactis and characterization of the gene hpt encoding hypoxanthine guanine phosphoribosyltransferase. Mol & Gen Genet 235:359–364
    [Google Scholar]
  18. Orengo A., Kobayashi S.-H. 1978; Uridine-cytidine kinase from Novikoff ascites rat tumor and Bacillus stearothermophilus. . Methods Enzymol 51:299–305
    [Google Scholar]
  19. Parks R.E. Jr Agarwal R.P. 1972; Purine nucleoside phosphorylase. In The Enzymes VII, 3. pp. 483–514 Boyer P. D. Edited by New York:: Academic Press.;
    [Google Scholar]
  20. Rasmussen U.B., Mygind B., Nygaard P. 1986; Purification and some properties of uracil phosphoribosyltransferase from Escherichia coli K-12. Biochim Biophys Acta 881:268–275
    [Google Scholar]
  21. Rumyantseva E.V., Sukhodelets V.V., Smirnov Y.V. 1979; Isolation and characterization of mutants for genes of nucleoside catabolism In Bacillus subtilis. . Genetica 15:594–604
    [Google Scholar]
  22. Schwartz M. 1978; Thymidine phosphorylase from Escherichia coli. . Methods Enzymol 51:442–445
    [Google Scholar]
  23. Terzaghi B.E., Sandine W.E. 1975; Improved medium for lactic streptococci and their bacteriophages. Appl Environ Microbiol 29:807–813
    [Google Scholar]
  24. Valentin-Hansen P. 1978; Uridine-cytidine kinase from Escherichia coli. . Methods Enzymol 51:308–314
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-141-8-1883
Loading
/content/journal/micro/10.1099/13500872-141-8-1883
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error