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Volume 53, Issue 3

gen. nov., sp. nov., a novel hydrocarbonoclastic marine bacterium isolated from Antarctic coastal sea water Free

Abstract

The taxonomic characteristics of two bacterial strains, RB-8 and RB-9, isolated from hydrocarbon-degrading enrichment cultures obtained from Antarctic coastal marine environments (Rod Bay, Ross Sea), were determined. These bacteria were psychrophilic, aerobic and Gram-negative with polar flagella. Growth was not observed in the absence of NaCl, occurred only at concentrations of Na above 20 mM and was optimal at an NaCl concentration of 3–5 % (w/v). The major cellular fatty acids were monounsaturated straight-chain fatty acids. The strains were able to synthesize the polyunsaturated fatty acid eicosapentaenoic acid (20 : 53) at low temperatures. The DNA G+C contents were 41–42 mol%. The strains formed a distinct phyletic line within the -, with less than 89·6 % sequence identity to their closest relatives within the with validly published names. Both isolates exhibited a restricted substrate profile, with a preference for aliphatic hydrocarbons, that is typical of marine hydrocarbonoclastic micro-organisms such as , and . On the basis of ecophysiological properties, G+C content, 16S rRNA gene sequences and fatty acid composition, a novel genus and species within the - are proposed, gen. nov., sp. nov.; strain RB-8 (=DSM 14852=LMG 21398) is the type strain.

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Keyword(s): PLFA, phospholipid fatty acid and PUFA, polyunsaturated fatty acid
SGM
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2003-05-01
2024-04-16
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Oleispira antarctica gen. nov., sp. nov., a novel hydrocarbonoclastic marine bacterium isolated from Antarctic coastal sea water
Int J Syst Evol Microbiol 53, 779 (2003); https://doi.org/10.1099/ijs.0.02366-0
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