1887

Abstract

Aster yellows (AY) group (16SrI) phytoplasmas are associated with over 100 economically important diseases worldwide and represent the most diverse and widespread phytoplasma group. Strains that belong to the AY group form a phylogenetically discrete subclade within the phytoplasma clade and are related most closely to the stolbur phytoplasma subclade, based on analysis of 16S rRNA gene sequences. AY subclade strains are related more closely to their culturable relatives, spp., than any other phytoplasmas known. Within the AY subclade, six distinct phylogenetic lineages were revealed. Congruent phylogenies obtained by analyses of gene and ribosomal protein () operon gene sequences further resolved the diversity among AY group phytoplasmas. Distinct phylogenetic lineages were identified by RFLP analysis of 16S rRNA, or gene sequences. Ten subgroups were differentiated, based on analysis of gene sequences. It is proposed that AY group phytoplasmas represent at least one novel taxon. Strain OAY, which is a member of subgroups 16SrI-B, I-B and I-B and is associated with evening primrose () virescence in Michigan, USA, was selected as the reference strain for the novel taxon ‘ Phytoplasma asteris’. A comprehensive database of diverse AY phytoplasma strains and their geographical distribution is presented.

Keyword(s): AY, aster yellows
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2004-07-01
2024-03-28
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References

  1. Ahrens U., Lorenz K.-H., Seemüller E. 1993; Genetic diversity among mycoplasmalike organisms associated with stone fruit diseases. Mol Plant–Microbe Interact 6:686–691 [CrossRef]
    [Google Scholar]
  2. Albouy J. 1966; Le problème des germes fins du gladieul. Ann Epiphyt 17:81–93 (in French
    [Google Scholar]
  3. Alminaite A., Davis R. E., Valiunas D., Jomantiene R. 2002; First report of a group 16SrI, subgroup B, phytoplasma in diseased Epilobium hirsutum in the region of Tallin, Estonia. Plant Dis 86:1177
    [Google Scholar]
  4. Berges R., Cousin M.-T., Roux J., Mäurer R., Seemüller E. 1997; Detection of phytoplasma infections in declining Populus nigra ‘Italica’ trees and molecular differentiation of the aster yellows phytoplasmas identified in various Populus species. Eur J For Pathol 27:33–43 [CrossRef]
    [Google Scholar]
  5. Bertaccini A., Davis R. E., Lee I.-M., Conti M., Dally E. L., Douglas S. M. 1990; Detection of chrysanthemum yellows mycoplasmalike organism by dot hybridization and Southern blot analysis. Plant Dis 74:40–43 [CrossRef]
    [Google Scholar]
  6. Bertaccini A., Mittempergher L., Vibio M. 1996; Identification of phytoplasmas associated with a decline of European hackberry ( Celtis australis . Ann Appl Biol 128:245–253 [CrossRef]
    [Google Scholar]
  7. Bertaccini A., Carraro L., Davies D. L., Laimer da Câmara Machado M., Martini M., Paltrinieri S., Seemüller E. 2000; Micropropagation of a collection of phytoplasma strains in periwinkle and other host plants. In Proceedings of the 13th International Congress of IOM, Fukuoka, Japan. July 14–19 2000 Abstract no. 101
    [Google Scholar]
  8. Borth W. B., Hamasaki R. T., Ogata D., Fukuda S. K., Hu J. S. 2002; First report of phytoplasmas infecting watercress in Hawaii. Plant Dis 86:331 [CrossRef]
    [Google Scholar]
  9. Brcák J. 1979; Leafhopper and planthopper vectors of plant disease agents in central and southern Europe. In Leafhopper Vectors and Plant Disease Agents pp  97–154 Edited by Maramorosch K., Harris K. F. New York: Academic Press;
    [Google Scholar]
  10. Chiykowski L. N. 1991; Vector–pathogen–host plant relationships of clover phyllody mycoplasmalike organism and the vector leafhopper Paraphlepsius irroratus . Can J Plant Pathol 13:11–18 [CrossRef]
    [Google Scholar]
  11. Cousin M. T., Sharma J., Rousseau J., Poitevin J.-P., Savoure A. 1986; Hydrangea virescence. I. Description of the disease and its transmission to the differential host plant Catharanthus roseus by Cuscuta subinclusa . Agron Sci Prod Veg Environ 6:249–254
    [Google Scholar]
  12. Danielli A., Bertaccini A., Vibio M., Rapetti S. 1996; Identificazione di fitoplasmi associati allo scopazzo dell' olivo. In Atti Convegno Annuale SIPaV (Società Italiana di Patologia Vegetale) pp  C28–C29 September 26–27 1996, Udine, Italy (in Italian
    [Google Scholar]
  13. Davis R. E., Lee I.-M., Douglas S. M., Dally E. L. 1990; Molecular cloning and detection of chromosomal and extrachromosomal DNA of the mycoplasmalike organism (MLO) associated with little leaf disease in periwinkle ( Catharanthus roseus ). Phytopathology 80:789–793 [CrossRef]
    [Google Scholar]
  14. Davis R. E., Dally E. L., Gundersen D. E., Lee I.-M., Habili N. 1997; Candidatus Phytoplasma australiense,” a new phytoplasma taxon associated with Australian grapevine yellows. Int J Syst Bacteriol 47:262–269 [CrossRef]
    [Google Scholar]
  15. Deng S., Hiruki C. 1991; Genetic relatedness between two nonculturable mycoplasmalike organisms revealed by nucleic acid hybridization and polymerase chain reaction. Phytopathology 81:1475–1479 [CrossRef]
    [Google Scholar]
  16. Faivre-Amiot A., Moreau J.-P., Cousin M.-T., Staron T. 1970; Essai de mise en culture de l'agent de la phyllodie du trèfle. Ann Phytopathol 2:251–258 (in French
    [Google Scholar]
  17. Griffiths H. M., Sinclair W. A., Davis R. E., Lee I.-M., Dally E. L., Guo Y.-H., Chen T. A., Hibben C. R. 1994; Characterization of mycoplasmalike organisms from Fraxinus , Syringa , and associated plants from geographically diverse sites. Phytopathology 84:119–126 [CrossRef]
    [Google Scholar]
  18. Griffiths H. M., Sinclair W. A., Smart C. D., Davis R. E. 1999; The phytoplasma associated with ash yellows and lilac witches'-broom: ‘ Candidatus Phytoplasma fraxini’. Int J Syst Bacteriol 49:1605–1614 [CrossRef]
    [Google Scholar]
  19. Gundersen D. E., Lee I.-M., Rehner S. A., Davis R. E., Kingsbury D. T. 1994; Phylogeny of mycoplasmalike organisms (phytoplasmas): a basis for their classification. J Bacteriol 176:5244–5254
    [Google Scholar]
  20. Gundersen D. E., Lee I.-M., Schaff D. A., Harrison N. A., Chang C. J., Davis R. E., Kingsbury D. T. 1996; Genomic diversity and differentiation among phytoplasma strains in 16S rRNA groups I (aster yellows and related phytoplasmas) and III (X-disease and related phytoplasmas). Int J Syst Bacteriol 46:64–75 [CrossRef]
    [Google Scholar]
  21. Harrison N. A., Richardson P. A., Tsai J. H., Ebbert M. A., Kramer J. B. 1996a; PCR assay for detection of the phytoplasma associated with maize bushy stunt disease. Plant Dis 80:263–269 [CrossRef]
    [Google Scholar]
  22. Harrison N. A., Tsai J. H., Richardson P. A. 1996b; Etiological and epidemiological investigations of periwinkle witches'-broom disease in Florida. IOM Lett 4:197–198
    [Google Scholar]
  23. IRPCM 2000; Report of the working team for phytoplasmas, spiroplasmas, mesoplasmas and entomoplasmas. In Report of Consultations of the International Research Program on Comparative Mycoplasmology (IRPCM) of the International Organization for Mycoplasmology (IOM) Fukuoka, Japan: IOM;
    [Google Scholar]
  24. Jomantiene R., Davis R. E., Maas J., Dally E. L. 1998; Classification of new phytoplasmas associated with diseases of strawberry in Florida, based on analysis of 16S rRNA and ribosomal protein gene operon sequences. Int J Syst Bacteriol 48:269–277 [CrossRef]
    [Google Scholar]
  25. Jomantiene R., Davis R. E., Antoniuk L., Staniulis J. 2000; First report of phytoplasmas in soybean, alfalfa, and Lupinus sp. in Lithuania. Plant Dis 84:198
    [Google Scholar]
  26. Jomantiene R., Davis R. E., Alminaite A., Valiunas D., Jasinskaite R. 2002a; First report of oat as host of a phytoplasma belonging to group 16SrI, subgroup A. Plant Dis 86:443
    [Google Scholar]
  27. Jomantiene R., Maas J. L., Takeda F., Davis R. E. 2002b; Molecular identification and classification of strawberry phylloid fruit phytoplasma in group 16SrI, new subgroup R. Plant Dis 86:920
    [Google Scholar]
  28. Jung H.-Y., Sawayanagi T., Kakizawa S. 7 other authors 2002; Candidatus Phytoplasma castaneae’, a novel phytoplasma taxon associated with chestnut witches' broom disease. Int J Syst Evol Microbiol 52:1543–1549 [CrossRef]
    [Google Scholar]
  29. Keane G., Edwards A., Clark M. F. 1996; Differentiation of group 16SrI-B aster yellows phytoplasmas with monoclonal antibodies. In BCPC Symposium Proceedings: Diagnostic in Crop Production. no 65 pp  263–268
    [Google Scholar]
  30. Kunkel L. O. 1926; Studies on aster yellows. Am J Bot 23:646–705
    [Google Scholar]
  31. Kunkel L. O. 1932; Celery yellows of California not identical with the aster yellows of New York. Contrib Boyce Thompson Inst 4:405–414
    [Google Scholar]
  32. Kuske C. R., Kirkpatrick B. C. 1992; Phylogenetic relationships between the western aster yellows mycoplasmalike organism and other prokaryotes established by 16S rRNA gene sequence. Int J Syst Bacteriol 42:226–233 [CrossRef]
    [Google Scholar]
  33. Lee I.-M., Davis R. E. 1988; Detection and investigation of genetic relatedness among aster yellows and other mycoplasma-like organisms by using cloned DNA and RNA probes. Mol Plant–Microbe Interact 1:303–310 [CrossRef]
    [Google Scholar]
  34. Lee I.-M., Davis R. E. 2000; Aster yellows. In Encyclopedia of Plant Pathology pp  60–63 Edited by Maloy O. C., Murray T. D. New York: Wiley;
    [Google Scholar]
  35. Lee I.-M., Davis R. E., Chen T.-A., Chiykowski L. N., Fletcher J., Hiruki C., Schaff D. A. 1992; A genotype-based system for identification and classification of mycoplasmalike organisms (MLOs) in the aster yellows MLO strain cluster. Phytopathology 82:977–986 [CrossRef]
    [Google Scholar]
  36. Lee I.-M., Davis R. E., Sinclair W. A., DeWitt N. D., Conti M. 1993a; Genetic relatedness of mycoplasmalike organisms detected in Ulmus spp. in the United States and Italy by means of DNA probes and polymerase chain reactions. Phytopathology 83:829–833 [CrossRef]
    [Google Scholar]
  37. Lee I.-M., Hammond R. W., Davis R. E., Gundersen D. E. 1993b; Universal amplification and analysis of pathogen 16S rDNA for classification and identification of mycoplasmalike organisms. Phytopathology 83:834–842 [CrossRef]
    [Google Scholar]
  38. Lee I.-M., Gundersen-Rindal D. E., Bertaccini A. 1998a; Phytoplasma: ecology and genomic diversity. Phytopathology 88:1359–1366 [CrossRef]
    [Google Scholar]
  39. Lee I.-M., Gundersen-Rindal D. E., Davis R. E., Bartoszyk I. M. 1998b; Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. Int J Syst Bacteriol 48:1153–1169 [CrossRef]
    [Google Scholar]
  40. Lee I.-M., Davis R. E., Gundersen-Rindal D. E. 2000; Phytoplasma: phytopathogenic mollicutes. Annu Rev Microbiol 54:221–255 [CrossRef]
    [Google Scholar]
  41. Lee M. E., Grau C. R., Lukaesko L. A., Lee I.-M. 2002; Identification of aster yellows phytoplasmas in soybean in Wisconsin based on RFLP analysis of PCR-amplified products (16S rDNAs. Can J Plant Pathol 24:125–130 [CrossRef]
    [Google Scholar]
  42. Lim P.-O., Sears B. B. 1989; 16S rRNA sequence indicates that plant-pathogenic mycoplasmalike organisms are evolutionarily distinct from animal mycoplasmas. J Bacteriol 171:5901–5906
    [Google Scholar]
  43. Lim P.-O., Sears B. B. 1992; Evolutionary relationships of a plant-pathogenic mycoplasmalike organism and Acholeplasma laidlawii deduced from two ribosomal protein gene sequences. J Bacteriol 174:2606–2611
    [Google Scholar]
  44. Lin C. P., Chen T.-A. 1985; Monoclonal antibodies against the aster yellows agent. Science 227:1233–1235 [CrossRef]
    [Google Scholar]
  45. Maixner M., Aherns U., Seemüller E. 1994; Detection of mycoplasmalike organisms associated with a yellows disease of grapevine in Germany. J Phytopathol 142:1–10 [CrossRef]
    [Google Scholar]
  46. Marcone C., Ragozzino A., Seemüller E. 1997a; Detection and identification of phytoplasmas in yellows-diseased weeds in Italy. Plant Pathol 46:530–537 [CrossRef]
    [Google Scholar]
  47. Marcone C., Ragozzino A., Seemüller E. 1997b; Detection and identification of phytoplasmas infecting vegetable, ornamental and forage crops in southern Italy. J Plant Pathol 79:211–217
    [Google Scholar]
  48. Marcone C., Ragozzino A., Seemüller E. 1997c; Detection and identification of phytoplasmas infecting wild plant species in southern Italy. In Proceedings of the 10th Congress of the Mediterranean Phytopathology Union pp  251–255 Montpellier: 1–5 June 1997
    [Google Scholar]
  49. Marcone C., Lee I.-M., Davis R. E., Ragozzino A., Seemüller E. 2000; Classification of aster yellows-group phytoplasmas based on combined analyses of rRNA and tuf gene sequences. Int J Syst Evol Microbiol 50:1703–1713
    [Google Scholar]
  50. Marwitz R. 1990; Diversity of yellows disease agents in plant infections. Zentbl Bakteriol Suppl 20:431–434
    [Google Scholar]
  51. McCoy R. E., Caudwell A., Chang C. J. 16 other authors 1989; Plant diseases associated with mycoplasma-like organisms. In The Mycoplasmas vol. 5 pp  545–640 Edited by Whitcomb R. F., Tully J. G. San Diego, CA: Academic Press;
    [Google Scholar]
  52. Montano H. G., Davis R. E., Dally E. L., Hogenhout S., Pimentel J. P., Brioso P. S. T. 2001; Candidatus Phytoplasma brasiliense’, a new phytoplasma taxon associated with hibiscus witches' broom disease. Int J Syst Evol Microbiol 51:1109–1118 [CrossRef]
    [Google Scholar]
  53. Murray R. G. E., Schleifer K. H. 1994; Taxonomic notes: a proposal for recording the properties of putative taxa of procaryotes. Int J Syst Bacteriol 44:174–176 [CrossRef]
    [Google Scholar]
  54. Namba S., Oyaizu H., Kato S., Iwanami S., Tsuchizaki T. 1993; Phylogenetic diversity of phytopathogenic mycoplasmalike organisms. Int J Syst Bacteriol 43:461–467 [CrossRef]
    [Google Scholar]
  55. Okuda S., Prince J. P., Davis R. E., Dally E. L., Lee I.-M., Morgen B., Kato S. 1997; Two groups of phytoplasmas from Japan distinguished on the basis of amplification and restriction analysis of 16S rDNA. Plant Dis 81:301–305 [CrossRef]
    [Google Scholar]
  56. Sawayanagi T., Horikoshi N., Kanehira T., Shinohara M., Bertaccini A., Cousin M.-T., Hiruki C., Namba S. 1999; Candidatus Phytoplasma japonicum’, a new phytoplasma taxon associated with Japanese Hydrangea phyllody. Int J Syst Bacteriol 49:1275–1285 [CrossRef]
    [Google Scholar]
  57. Schneider B., Ahrens U., Kirkpatrick B. C., Seemüller E. 1993; Classification of plant-pathogenic mycoplasma-like organisms using restriction-site analysis of PCR-amplified 16S rDNA. J Gen Microbiol 139:519–527 [CrossRef]
    [Google Scholar]
  58. Schneider B., Seemüller E., Smart C. D., Kirkpatrick B. C. 1995; Phylogenetic classification of plant pathogenic mycoplasma-like organisms or phytoplasmas. In Molecular and Diagnostic Procedures in Mycoplasmology vol 1 pp  369–380 Edited by Razin S., Tully J. G. San Diego, CA: Academic Press;
    [Google Scholar]
  59. Schneider B., Marcone C., Kampmann M., Ragozzino A., Lederer W., Cousin M.-T., Seemüller E. 1997; Characterization and classification of phytoplasmas from wild and cultivated plants by RFLP and sequence analysis of ribosomal DNA. Eur J Plant Pathol 103:675–686 [CrossRef]
    [Google Scholar]
  60. Sears B. B., Lim P.-O., Holland N., Kirkpatrick B. C., Klomparens K. L. 1989; Isolation and characterization of DNA from a mycoplasmalike organism. Mol Plant–Microbe Interact 2:175–180 [CrossRef]
    [Google Scholar]
  61. Seemüller E., Schneider B., Mäurer R. 8 other authors 1994; Phylogenetic classification of phytopathogenic mollicutes by sequence analysis of 16S ribosomal DNA. Int J Syst Bacteriol 44:440–446 [CrossRef]
    [Google Scholar]
  62. Seemüller E., Marcone C., Lauer U., Ragozzino A., Göschl M. 1998; Current status of molecular classification of the phytoplasmas. J Plant Pathol 80:3–26
    [Google Scholar]
  63. Stanosz G. R., Heimann M. F., Lee I.-M. 1997; Purple coneflower is a host of the aster yellows phytoplasma. Plant Dis 81:424
    [Google Scholar]
  64. Tsai J. H. 1979; Vector transmission of mycoplasmal agents of plant diseases. In The Mycoplasmas vol. 3 pp  266–307 Edited by Whitcomb R. E., Tully J. G. San Diego, CA: Academic Press;
    [Google Scholar]
  65. Valiunas D. 2003; Identification of phytoplasmas in Lithuania and estimation of their biodiversity and molecular evolutionary relationships . PhD thesis Institute of Botany; Vilnius, Lithuania:
  66. Valiunas D., Alminaite A., Staniulis J., Jomantiene R., Davis R. E. 2001; First report of aster yellows-related subgroup I-A phytoplasma strains in carrot, phlox, sea-lavender, aconitum, and hyacinth in Lithuania. Plant Dis 85:804
    [Google Scholar]
  67. Van Slogteren D. H. M., Groen N. P. A., Muller P. J. 1974; Yellow disease of gladiolus and hyacinth in the Netherlands. 1. Additional data on field control with systemic insecticides and effects of treating corms and bulbs in hot water. 2. Detection of mycoplasma-like bodies in the phloem of diseased hyacinth leaves. Acta Hortic 36:303–311
    [Google Scholar]
  68. Verdin E., Salar P., Danet J.-L., Choueiri E., Jreijiri F., El Zammar S., Gélie B, Bové J. M., Garnier M. 2003; Candidatus Phytoplasma phoenicium’, a novel phytoplasma associated with an emerging lethal disease of almond trees in Lebanon and Iran. Int J Syst Evol Microbiol 53:833–838 [CrossRef]
    [Google Scholar]
  69. Vibio M., Bertaccini A., Lee I.-M., Davis R. E., Minucci C., Milne R. G. 1994; Etiology of lettuce yellows in Italy: genetic characterization of associated mycoplasmalike organisms. Phytopathol Mediterr 33:179–186
    [Google Scholar]
  70. Vibio M., Bertaccini A., Schiliro E., Rapetti S. 1995; Caratterizzazione molecolare di fitoplasmi associati a virescenza in anemone. Informatore Fitopatologico 45:60–62 (in Italian
    [Google Scholar]
  71. Vibio M., Bertaccini A., Lee I.-M., Davis R. E., Clark M. F. 1996; Differentiation and classification of aster yellows and related European phytoplasmas. Phytopathol Mediterr 35:33–42
    [Google Scholar]
  72. Villalobos W., Moreira L., Rivera C., Bottner K. D., Lee I.-M. 2002; First report of an aster yellows subgroup 16SrI-B phytoplasma infecting chayote in Costa Rica. Plant Dis 86:330
    [Google Scholar]
  73. Wang K., Hiruki C. 2001; Molecular characterization and classification of phytoplasmas associated with canola yellows and a new phytoplasma strain associated with dandelions. Plant Dis 85:76–79 [CrossRef]
    [Google Scholar]
  74. White D. T., Blackall L. L., Scott P. T., Walsh K. B. 1998; Phylogenetic positions of phytoplasmas associated with dieback, yellow crinkle and mosaic diseases of papaya, and their proposed inclusion in ‘ Candidatus Phytoplasma australiense’ and a new taxon, ‘ Candidatus Phytoplasma australasia’. Int J Syst Bacteriol 48:941–951 [CrossRef]
    [Google Scholar]
  75. Zreik L., Carle P., Bové J. M., Garnier M. 1995; Characterization of the mycoplasmalike organism associated with witches'-broom disease of lime and proposition of a “ Candidatus ” taxon for the organism, “ Candidatus Phytoplasma aurantifolia”. Int J Syst Bacteriol 45:449–453 [CrossRef]
    [Google Scholar]
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