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Volume 64, Issue Pt_5

gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of fam. nov. Free

Abstract

The taxonomic characteristics of two bacterial strains, FH5 and SS4, isolated from enrichment cultures obtained from two distinct marine environments, were determined. These bacteria were Gram-stain-negative, facultatively anaerobic rods. Growth occurred at 20–40 °C (optimum, 28–32 °C), pH 5.5–9.0 (optimum, pH 7.0–7.5) and in the presence of 1–7 % NaCl (optimum, 2–4 %). The major cellular fatty acids were anteiso-C and iso-C. Menaquinone 7 (MK-7) was the sole respiratory quinone. The major polar lipids were phosphatidylethanolamine, an unkown phospholipid and an unknown lipid. The DNA G+C contents of strains FH5 and SS4 were both determined to be 42.0 mol%. The results of DNA–DNA hybridization studies indicated that the FH5 and SS4 genomes share greater than 95 % relatedness. The strains formed a distinct phyletic line within the class , with less than 89.4 % sequence similarity to their closest relatives with validly published names. On the basis of physiological and biochemical characteristics, 16S rRNA gene sequences and chemical properties, a novel genus and species, gen. nov., sp. nov., within the class , are proposed, with strain FH5 ( = DSM 25947 = CICC 10585) as the type strain. In addition, a new family, fam. nov., is proposed to accommodate gen. nov.

  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31290231 and 31370057)
  • National Science and Technology Major Project of China (Award 2013ZX10004217)
  • China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (Award DY125-15-T-05)
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2014-05-01
2024-04-24
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References

  1. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 1861–1868.[PubMed]
    [Google Scholar]
  2. Chassard C., Delmas E., Lawson P. A., Bernalier-Donadille A. ( 2008 ). Bacteroides xylanisolvens sp. nov., a xylan-degrading bacterium isolated from human faeces. . Int J Syst Evol Microbiol 58, 1008–1013. [View Article] [PubMed]
    [Google Scholar]
  3. CLSI ( 2012 ). Performance Standards for Antimicrobial Susceptibility Testing; 22nd Informational Supplement M100-S22 . Wayne, PA:: Clinical and Laboratory Standards Institute;.
     [Google Scholar]
  4. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
     [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133–142. [View Article] [PubMed]
    [Google Scholar]
  6. Gonzalez J. M., Saiz-Jimenez C. ( 2002 ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4, 770–773. [View Article] [PubMed]
    [Google Scholar]
  7. Gonzalez J. M., Saiz-Jimenez C. ( 2005 ). A simple fluorimetric method for the estimation of DNA-DNA relatedness between closely related microorganisms by thermal denaturation temperatures. . Extremophiles 9, 75–79. [View Article] [PubMed]
    [Google Scholar]
  8. Hayashi H., Shibata K., Bakir M. A., Sakamoto M., Tomita S., Benno Y. ( 2007 ). Bacteroides coprophilus sp. nov., isolated from human faeces. . Int J Syst Evol Microbiol 57, 1323–1326. [View Article] [PubMed]
    [Google Scholar]
  9. Holmes D. E., Nevin K. P., Woodard T. L., Peacock A. D., Lovley D. R. ( 2007 ). Prolixibacter bellariivorans gen. nov., sp. nov., a sugar-fermenting, psychrotolerant anaerobe of the phylum Bacteroidetes, isolated from a marine-sediment fuel cell. . Int J Syst Evol Microbiol 57, 701–707. [View Article] [PubMed]
    [Google Scholar]
  10. Huang X.-F., Liu Y.-J., Dong J.-D., Qu L.-Y., Zhang Y.-Y., Wang F.-Z., Tian X.-P., Zhang S. ( 2013 ). Mangrovibacterium diazotrophicum gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from a mangrove sediment, and proposal of Prolixibacteraceae fam. nov. . Int J Syst Evol Microbiol (in press). [View Article] [PubMed]
    [Google Scholar]
  11. Jordan E. M., Thompson F. L., Zhang X.-H., Li Y., Vancanneyt M., Kroppenstedt R. M., Priest F. G., Austin B. ( 2007 ). Sneathiella chinensis gen. nov., sp. nov., a novel marine alphaproteobacterium isolated from coastal sediment in Qingdao, China. . Int J Syst Evol Microbiol 57, 114–121. [View Article] [PubMed]
    [Google Scholar]
  12. Kim M. S., Roh S. W., Bae J. W. ( 2010 ). Bacteroides faecis sp. nov., isolated from human faeces. . Int J Syst Evol Microbiol 60, 2572–2576. [View Article] [PubMed]
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  14. Kitahara M., Tsuchida S., Kawasumi K., Amao H., Sakamoto M., Benno Y., Ohkuma M. ( 2011 ). Bacteroides chinchillae sp. nov. and Bacteroides rodentium sp. nov., isolated from chinchilla (Chinchilla lanigera) faeces. . Int J Syst Evol Microbiol 61, 877–881. [View Article] [PubMed]
    [Google Scholar]
  15. Kitahara M., Sakamoto M., Tsuchida S., Kawasumi K., Amao H., Benno Y., Ohkuma M. ( 2012 ). Bacteroides stercorirosoris sp. nov. and Bacteroides faecichinchillae sp. nov., isolated from chinchilla (Chinchilla lanigera) faeces. . Int J Syst Evol Microbiol 62, 1145–1150. [View Article] [PubMed]
    [Google Scholar]
  16. Krieg N. R. ( 2011 ). Class I. Bacteroidia class. nov.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 25. Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B. . New York:: Springer;.
     [Google Scholar]
  17. Kroppenstedt R. M. ( 1982 ). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. . J Liq Chromatogr 5, 2359–2367. [View Article]
    [Google Scholar]
  18. Lan P. T. N., Sakamoto M., Sakata S., Benno Y. ( 2006 ). Bacteroides barnesiae sp. nov., Bacteroides salanitronis sp. nov. and Bacteroides gallinarum sp. nov., isolated from chicken caecum. . Int J Syst Evol Microbiol 56, 2853–2859. [View Article] [PubMed]
    [Google Scholar]
  19. Na H., Kim S., Moon E. Y., Chun J. ( 2009 ). Marinifilum fragile gen. nov., sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 59, 2241–2246. [View Article] [PubMed]
    [Google Scholar]
  20. Nishiyama T., Ueki A., Kaku N., Watanabe K., Ueki K. ( 2009 ). Bacteroides graminisolvens sp. nov., a xylanolytic anaerobe isolated from a methanogenic reactor treating cattle waste. . Int J Syst Evol Microbiol 59, 1901–1907. [View Article] [PubMed]
    [Google Scholar]
  21. Qu L., Zhu F., Hong X., Gao W., Chen J., Sun X. ( 2011 ). Sunxiuqinia elliptica gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment in a sea cucumber farm. . Int J Syst Evol Microbiol 61, 2885–2889. [View Article] [PubMed]
    [Google Scholar]
  22. Robert C., Chassard C., Lawson P. A., Bernalier-Donadille A. ( 2007 ). Bacteroides cellulosilyticus sp. nov., a cellulolytic bacterium from the human gut microbial community. . Int J Syst Evol Microbiol 57, 1516–1520. [View Article] [PubMed]
    [Google Scholar]
  23. Rosselló-Mora R., Amann R. ( 2001 ). The species concept for prokaryotes. . FEMS Microbiol Rev 25, 39–67. [View Article] [PubMed]
    [Google Scholar]
  24. Ruvira M. A., Lucena T., Pujalte M. J., Arahal D. R., Macián M. C. ( 2013 ). Marinifilum flexuosum sp. nov., a new Bacteroidetes isolated from coastal Mediterranean Sea water and emended description of the genus Marinifilum Na et al., 2009. . Syst Appl Microbiol 36, 155–159. [View Article] [PubMed]
    [Google Scholar]
  25. Sakamoto M., Ohkuma M. ( 2013 ). Bacteroides reticulotermitis sp. nov., isolated from the gut of a subterranean termite (Reticulitermes speratus). . Int J Syst Evol Microbiol 63, 691–695. [View Article] [PubMed]
    [Google Scholar]
  26. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, Technical Note 101. Newark, DE:: Microbial MIDI Inc;.
     [Google Scholar]
  27. Shalley S., Pradip Kumar S., Srinivas T. N., Suresh K., Anil Kumar P. ( 2013 ). Marinilabilia nitratireducens sp. nov., a lipolytic bacterium of the family Marinilabiliaceae isolated from marine solar saltern. . Antonie van Leeuwenhoek 103, 519–525. [View Article] [PubMed]
    [Google Scholar]
  28. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  29. Takai K., Abe M., Miyazaki M., Koide O., Nunoura T., Imachi H., Inagaki F., Kobayashi T. ( 2013 ). Sunxiuqinia faeciviva sp. nov., a facultatively anaerobic organoheterotroph of the Bacteroidetes isolated from deep subseafloor sediment. . Int J Syst Evol Microbiol 63, 1602–1609. [View Article] [PubMed]
    [Google Scholar]
  30. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  31. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 4876–4882. [View Article] [PubMed]
    [Google Scholar]
  32. Ueki A., Abe K., Kaku N., Watanabe K., Ueki K. ( 2008 ). Bacteroides propionicifaciens sp. nov., isolated from rice-straw residue in a methanogenic reactor treating waste from cattle farms. . Int J Syst Evol Microbiol 58, 346–352. [View Article] [PubMed]
    [Google Scholar]
  33. Ueki A., Abe K., Ohtaki Y., Kaku N., Watanabe K., Ueki K. ( 2011 ). Bacteroides paurosaccharolyticus sp. nov., isolated from a methanogenic reactor treating waste from cattle farms. . Int J Syst Evol Microbiol 61, 448–453. [View Article] [PubMed]
    [Google Scholar]
  34. Watanabe Y., Nagai F., Morotomi M., Sakon H., Tanaka R. ( 2010 ). Bacteroides clarus sp. nov., Bacteroides fluxus sp. nov. and Bacteroides oleiciplenus sp. nov., isolated from human faeces. . Int J Syst Evol Microbiol 60, 1864–1869. [View Article] [PubMed]
    [Google Scholar]
  35. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
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Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov.
Int J Syst Evol Microbiol 64, 1690 (2014); https://doi.org/10.1099/ijs.0.056812-0
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