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Volume 64, Issue Pt_12

sp. nov., isolated from baboons ( spp.) Free

Abstract

Two Gram-negative, non-motile, non-spore-forming coccoid bacteria (strains F8/08-60 and F8/08-61) isolated from clinical specimens obtained from baboons ( spp.) that had delivered stillborn offspring were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence similarities, both strains, which possessed identical sequences, were assigned to the genus . This placement was confirmed by extended multilocus sequence analysis (MLSA), where both strains possessed identical sequences, and whole-genome sequencing of a representative isolate. All of the above analyses suggested that the two strains represent a novel lineage within the genus . The strains also possessed a unique profile when subjected to the phenotyping approach classically used to separate species of the genus reacting only with A monospecific antiserum, being sensitive to the dyes thionin and fuchsin, being lysed by bacteriophage Wb, Bk and Fi phage at routine test dilution (RTD) but only partially sensitive to bacteriophage Tb, and with no requirement for CO and no production of HS but strong urease activity. Biochemical profiling revealed a pattern of enzyme activity and metabolic capabilities distinct from existing species of the genus . Molecular analysis of the locus genes showed that both strains had a novel combination of two highly similar gene copies. The two strains shared a unique fingerprint profile of the multiple-copy -specific element IS Like MLSA, a multilocus variable number of tandem repeat analysis (MLVA) showed that the isolates clustered together very closely, but represent a distinct group within the genus . Isolates F8/08-60 and F8/08-61 could be distinguished clearly from all known species of the genus and their biovars by both phenotypic and molecular properties. Therefore, by applying the species concept for the genus suggested by the ICSP Subcommittee on the Taxonomy of , they represent a novel species within the genus , for which the name sp. nov. is proposed, with the type strain F8/08-60 ( = NCTC 13660 = CIRMBP 0958).

  • Published Online:
Funding
This study was supported by the:
  • South-west National Primate Research Center (Award P51 RR013986)
  • National Center for Research Resources, National Institutes of Health
  • Office of Research Infrastructure Programs (ORIP) of the National Institutes of Health (Award 1 C06 RR015456, 1 C06 RR014578 and P51 OD011133)
  • Federal Institute for Risk Assessment (Award BfR project no. 1322-503)
  • Direction Générale de l’Armement (Award Brucella REI2010 34 0003)
  • UK Department of Environment, Food and Rural Affairs (Defra)
Crown Copyright 2014. Reproduced with the permission of the Controller of Her Majesty's Stationery Office/Queen's Printer for Scotland and AHVLA.
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2014-12-01
2024-04-16
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Brucella papionis sp. nov., isolated from baboons (Papio spp.)
Int J Syst Evol Microbiol 64, 4120 (2014); https://doi.org/10.1099/ijs.0.065482-0
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