1887

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Volume 96, Issue 3

Diagnosis and discovery of fungal viruses using deep sequencing of small RNAs Free

Abstract

Analysis of virus-derived small RNAs with high-throughput sequencing has been successful for detecting novel viruses in plants and invertebrates. However, the applicability of this method has not been demonstrated in fungi, although fungi were among the first organisms reported to utilize RNA silencing. Here, we used virus-infected isolates of the fungal species complex as a model system to test whether mycovirus genome segments can be detected with small RNA deep sequencing. Species of the genus are some of the most devastating forest pathogens in boreal forests. These fungi cause wood decay and are commonly infected with species of the family and the unassigned virus species . Small RNA deep sequencing allowed the simultaneous detection of all eight double-stranded RNA virus strains known to be present in the tested samples and one putative mitovirus species (family ) with a single-stranded RNA genome, designated here as . Prior to this study, no members of the family had been described as infecting species of . Quantification of viral double- and single-stranded RNA with quantitative PCR indicated that co-infecting viral species and viruses with segmented genomes can be detected with small RNA deep sequencing despite vast differences in the amount of RNA. This is the first study demonstrating the usefulness of this method for detecting fungal viruses. Moreover, the results suggest that viral genomes are processed into small RNAs by different species of .

  • Received:
  • Accepted:
  • Published Online:
© 2015 The Authors
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2015-03-01
2024-04-18
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Diagnosis and discovery of fungal viruses using deep sequencing of small RNAs
J. Gen. Virol. 96, 714 (2015); https://doi.org/10.1099/jgv.0.000003
/content/journal/jgv/10.1099/jgv.0.000003
/content/journal/jgv/10.1099/jgv.0.000003
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