1887

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Volume 58, Issue 7

Leukocyte populations and cytokine expression in the mammary gland in a mouse model of mastitis Free

Abstract

is a contagious, mastitis-causing pathogen that is highly adapted to survive in the bovine mammary gland. This study used a BALB/c mouse model of mastitis to evaluate leukocyte populations in regional lymph nodes and cytokine expression in the mammary gland involved in the immune response against . It was found that the bacteria replicated efficiently in the mammary gland, peaking after 24 h and increasing by 100-fold. Dissemination of bacteria to systemic organs was observed 6 h after infection. At the same time, a massive infiltration of polymorphonuclear cells and an increase in the inflammatory cytokines interleukin (IL)-1, IL-6 and tumour necrosis factor- were detected in mammary glands, indicating an early inflammatory response. A decrease in the levels of inflammatory cytokines in mammary glands was observed 72 h after infection, accompanied by an increase in the levels of IL-12 and IL-10, which were related to a gradual decrease in bacterial load. An increase in the number of macrophages and B220 lymphocytes and similar increases in both CD4 and CD8 T cells in regional lymph nodes were observed, being most pronounced 5 days after infection. Moreover, increased levels of anti- antibodies in the mammary gland were observed 10 days after infection. Overall, these data suggest that the host exhibits both innate and acquired immune responses in response to mastitis.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): H&E, haematoxylin and eosin , IFN-γ, gamma interferon , IL, interleukin , MHC, major histocompatibility complex , PE, phycoerythrin , PMN, polymorphonuclear cell and TNF-α, tumour necrosis factor-α
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2009-07-01
2024-04-26
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References

  1. Almeida R. A., Patel D., Friton G. M., Oliver S. P. 2007; Intracellular killing of mastitis pathogens by penethamate hydriodide following internalization into mammary epithelial cells. J Vet Pharmacol Ther 30:151–156 [CrossRef]
     [Google Scholar]
  2. Armstrong L., Jordan N., Millar A. 1996; Interleukin 10 (IL-10) regulation of tumour necrosis factor alpha (TNF- α ) from human alveolar macrophages and peripheral blood monocytes. Thorax 51:143–149 [CrossRef]
     [Google Scholar]
  3. Bannerman D. D., Paape M. J., Lee J. W., Zhao X., Hope J. C., Rainard P. 2004; Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection. Clin Diagn Lab Immunol 11:463–472
     [Google Scholar]
  4. Berry E. A., Hillerton J. E. 2002; The effect of selective dry cow treatment on new intramammary infections. J Dairy Sci 85:112–121 [CrossRef]
     [Google Scholar]
  5. Brouillette E., Malouin F. 2005; The pathogenesis and control of Staphylococcus aureus -induced mastitis: study models in the mouse. Microbes Infect 7:560–568 [CrossRef]
     [Google Scholar]
  6. Brouillette E., Grondin G., Lefebvre C., Talbot B. G., Malouin F. 2004; Mouse mastitis model of infection for antimicrobial compound efficacy studies against intracellular and extracellular forms of Staphylococcus aureus . Vet Microbiol 101:253–262 [CrossRef]
     [Google Scholar]
  7. Cassatella M. A., Meda L., Bonora S., Ceska M., Constantin G. 1993; Interleukin 10 (IL-10) inhibits the release of proinflammatory cytokines from human polymorphonuclear leukocytes. Evidence for an autocrine role of tumor necrosis factor and IL-1 β in mediating the production of IL-8 triggered by lipopolysaccharide. J Exp Med 178:2207–2211 [CrossRef]
     [Google Scholar]
  8. Chandler R. L. 1970; Experimental bacterial mastitis in the mouse. J Med Microbiol 3:273–282 [CrossRef]
     [Google Scholar]
  9. Hertting O., Khalil A., Jaremko G., Chromek M., Li Y. H., Bakhiet M., Bartfai T., Tullus K., Brauner A. 2003; Enhanced chemokine response in experimental acute Escherichia coli pyelonephritis in IL-1 β -deficient mice. Clin Exp Immunol 131:225–233 [CrossRef]
     [Google Scholar]
  10. Hornef M. W., Wick M. J., Rhen M., Normark S. 2002; Bacterial strategies for overcoming host innate and adaptive immune responses. Nat Immunol 3:1033–1040 [CrossRef]
     [Google Scholar]
  11. Keefe G. P. 1997; Streptococcus agalactiae mastitis: a review. Can Vet J 38:429–437
     [Google Scholar]
  12. Keefe G. P., Dohoo I. R., Spangler E. 1997; Herd prevalence and incidence of Streptococcus agalactiae in the dairy industry of Prince Edward Island. J Dairy Sci 80:464–470 [CrossRef]
     [Google Scholar]
  13. Kotwal G. J. 1997; Microorganisms and their interaction with the immune system. J Leukoc Biol 62:415–429
     [Google Scholar]
  14. Leitner G., Yadlin B., Glickman A., Chaffer M., Saran A. 2000; Systemic and local immune response of cows to intramammary infection with Staphylococcus aureus . Res Vet Sci 69:181–184 [CrossRef]
     [Google Scholar]
  15. Logan E. F., Mackie D. P., Meneely D. J. 1984; Immunological features of consecutive intramammary infections with Streptococcus agalactiae in vaccinated and non-vaccinated heifers. Br Vet J 140:535–542 [CrossRef]
     [Google Scholar]
  16. Logan E. F., Mackie D. P., Meneely D. J. 1986; Distribution of antibody in different immunoglobulin classes in experimental Streptococcus agalactiae infection of the bovine udder. Br Vet J 142:358–363 [CrossRef]
     [Google Scholar]
  17. Mackie D. P., Logan E. F. 1986; Changes in immunoglobulin levels in whey during experimental Streptococcus agalactiae mastitis. Res Vet Sci 40:183–188
     [Google Scholar]
  18. Maxie M. G. (editor) 2007 Jubb, Kennedy and Palmer's Pathology of Domestic Animals , 5th edn. Philadelphia: Saunders;
     [Google Scholar]
  19. Merl K., Abdulmawjood A., Lammler C., Zschock M. 2003; Determination of epidemiological relationships of Streptococcus agalactiae isolated from bovine mastitis. FEMS Microbiol Lett 226:87–92 [CrossRef]
     [Google Scholar]
  20. Nakajima Y., Mikami O., Yoshioka M., Motoi Y., Ito T., Ishikawa Y., Fuse M., Nakano K., Yasukawa K. 1997; Elevated levels of tumor necrosis factor-alpha (TNF- α ) and interleukin-6 (IL-6) activities in the sera and milk of cows with naturally occurring coliform mastitis. Res Vet Sci 62:297–298 [CrossRef]
     [Google Scholar]
  21. Notebaert S., Meyer E. 2006; Mouse models to study the pathogenesis and control of bovine mastitis. A review. Vet Q 28:2–13 [CrossRef]
     [Google Scholar]
  22. Rambeaud M., Almeida R. A., Pighetti G. M., Oliver S. P. 2003; Dynamics of leukocytes and cytokines during experimentally induced Streptococcus uberis mastitis. Vet Immunol Immunopathol 96:193–205 [CrossRef]
     [Google Scholar]
  23. Rubens C. E., Smith S., Hulse M., Chi E. Y., van Belle G. 1992; Respiratory epithelial cell invasion by group B streptococci. Infect Immun 60:5157–5163
     [Google Scholar]
  24. Shuster D. E., Kehrli M. E. Jr, Stevens M. G. 1993; Cytokine production during endotoxin-induced mastitis in lactating dairy cows. Am J Vet Res 54:80–85
     [Google Scholar]
  25. Soltys J., Quinn M. T. 1999; Selective recruitment of T-cell subsets to the udder during staphylococcal and streptococcal mastitis: analysis of lymphocyte subsets and adhesion molecule expression. Infect Immun 67:6293–6302
     [Google Scholar]
  26. Spits H., de Waal Malefyt R. 1992; Functional characterization of human IL-10. Int Arch Allergy Immunol 99:8–15 [CrossRef]
     [Google Scholar]
  27. Tavares D., Ferreira P., Vilanova M., Videira A., Arala-Chaves M. 1995; Immunoprotection against systemic candidiasis in mice. Int Immunol 7:785–796 [CrossRef]
     [Google Scholar]
  28. Wilson C. D., Richards M. S. 1980; A survey of mastitis in the British dairy herd. Vet Rec 106:431–435 [CrossRef]
     [Google Scholar]
  29. Yamanaka H., Hisaeda K., Hagiwara K., Kirisawa R., Iwai H. 2000; ELISA for bovine interleukin-1 receptor antagonist and its application to mastitic sera and whey. J Vet Med Sci 62:661–664 [CrossRef]
     [Google Scholar]
  30. Zhu Y., Osterlundh I., Hulten F., Magnusson U. 2004; Tumor necrosis factor- α , interleukin-6, serum amyloid A, haptoglobin, and cortisol concentrations in sows following intramammary inoculation of Escherichia coli . Am J Vet Res 65:1434–1439 [CrossRef]
     [Google Scholar]
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Leukocyte populations and cytokine expression in the mammary gland in a mouse model of Streptococcus agalactiae mastitis
J. Med. Microbiol. 58, 951 (2009); https://doi.org/10.1099/jmm.0.007385-0
/content/journal/jmm/10.1099/jmm.0.007385-0
/content/journal/jmm/10.1099/jmm.0.007385-0
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