1887

Abstract

and are known to be associated with the development of dental caries. In this study these bacteria were detected in pre-school children (each with primary dentition, age range 3–5 years, = 60) using a PCR method, and then their presence was compared with the incidence of dental caries over a 1-year period. Plaque samples were collected from all erupted tooth sites using a sterile toothbrush. Dental examinations at the beginning of the study (baseline) and after 1 year were also performed to determine decayed, missing, filled teeth (dmft) scores using WHO caries diagnostic criteria. The prevalences of and across all the subjects were 61.7 % and 56.6 %, respectively; 13 subjects (21.7 %) were positive for alone, 10 (16.6 %) were positive for alone and 24 (40.0 %) were positive for both and , whereas 13 (21.7 %) were negative for both and . dmft scores of subjects positive for both and at baseline and after 1 year were significantly higher than of those positive for alone at the same stages ( < 0.01 and < 0.001, respectively). The caries incremental increase was also significantly greater in those with both bacteria detected ( < 0.05). Our results indicate that pre-school children harbouring both and have a significantly higher incidence of dental caries than those with alone.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46069-0
2005-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/54/7/JM540707.html?itemId=/content/journal/jmm/10.1099/jmm.0.46069-0&mimeType=html&fmt=ahah

References

  1. Alaluusua S., Alaluusua S. J., Karjalainen J. & 7 other authors; 1994; The demonstration by ribotyping of the stability of oral Streptococcus mutans infection over 5 to 7 years in children. Arch Oral Biol 39:467–471 [CrossRef]
    [Google Scholar]
  2. Carlsson P., Gandour I. A., Olsson B., Rickardsson B., Abbas K. 1987; High prevalence of mutans streptococci in a population with extremely low prevalence of dental caries. Oral Microbiol Immunol 2:121–124 [CrossRef]
    [Google Scholar]
  3. Caufield P. W., Cutter G. R., Dasanayake A. P. 1993; Initial acquisition of mutans streptococci by infants: evidence for a discrete window of infectivity. J Dent Res 72:37–45 [CrossRef]
    [Google Scholar]
  4. de Soet J. J., van Dalen P. J., Pavicic M. J. A. M. P., de Graaff J. 1990; Enumeration of mutans streptococci in clinical samples by using monoclonal antibodies. J Clin Microbiol 28:2467–2472
    [Google Scholar]
  5. Fujiwara T., Sasada E., Mima N., Ooshima T. 1991; Caries prevalence and salivary mutans streptococci in 0–2-year-old children of Japan. Community Dent Oral Epidemiol 19:151–154 [CrossRef]
    [Google Scholar]
  6. Goncharoff P., Figurski D. H., Stevens R. H., Fine D. H. 1993; Identification of Actinobacillus actinomycetemcomitans : polymerase chain reaction amplification of lktA -specific sequences. Oral Microbiol Immunol 8:105–110 [CrossRef]
    [Google Scholar]
  7. Hamada S., Slade H. D. 1980; Biology, immunology, and cariogenicity of Streptococcus mutans . Microbiol Rev 44:331–384
    [Google Scholar]
  8. Hirose H., Hirose K., Isogai E., Miura H., Ueda I. 1993; Close association between Streptococcus sobrinus in the saliva of young children and smooth-surface caries increment. Caries Res 27:292–297 [CrossRef]
    [Google Scholar]
  9. Igarashi T., Yamamoto A., Goto N. 1996; Direct detection of Streptococcus mutans in human dental plaque by polymerase chain reaction. Oral Microbiol Immunol 11:294–298 [CrossRef]
    [Google Scholar]
  10. Igarashi T., Yamamoto A., Goto N. 2000; PCR for detection and identification of Streptococcus sobrinus . J Med Microbiol 49:1069–1074
    [Google Scholar]
  11. Jordan H. V. 1986; Cultural methods for the identification and quantitation of Streptococcus mutans and lactobacilli in oral samples. Oral Microbiol Immunol 1:23–30 [CrossRef]
    [Google Scholar]
  12. Köhler B., Andréen I. 1994; Influence of caries-preventive measures in mothers on cariogenic bacteria and caries experience in their children. Arch Oral Biol 39:907–911 [CrossRef]
    [Google Scholar]
  13. Köhler B., Pettersson B. M., Bratthall D. 1981; Streptococcus mutans in plaque and saliva and the development of caries. Scand J Dent Res 89:19–25
    [Google Scholar]
  14. Köhler B., Andréen I., Jonsson B. 1988; The earlier the colonization by mutans streptococci, the higher the caries prevalence at 4 years of age. Oral Microbiol Immunol 3:14–17 [CrossRef]
    [Google Scholar]
  15. Köhler B., Bjarnason S., Care R., Mackevica I., Rence I. 1995a; Mutans streptococci and dental caries prevalence in a group of Latvian preschool children. Eur J Oral Sci 103:264–266 [CrossRef]
    [Google Scholar]
  16. Köhler B., Bjarnason S., Finnbogason S. Y., Holbrook W. P. 1995b; Mutans streptococci, lactobacilli and caries experience in 12-year-old Icelandic urban children, 1984 and 1991. Community Dent Oral Epidemiol 23:65–68 [CrossRef]
    [Google Scholar]
  17. Li Y., Caufield P. W. 1995; The fidelity of initial acquisition of mutans streptococci by infants from their mothers. J Dent Res 74:681–685 [CrossRef]
    [Google Scholar]
  18. Li Y., Navia J. M., Caufield P. W. 1994; Colonization by mutans streptococci in the mouths of 3- and 4-year-old Chinese children with or without enamel hypoplasia. Arch Oral Biol 39:1057–1062 [CrossRef]
    [Google Scholar]
  19. Loesche W. J. 1986; Role of Streptococcus mutans in human dental decay. Microbiol Rev 50:353–380
    [Google Scholar]
  20. Masuda N., Tsutsumi N., Sobue S., Hamada S. 1979; Longitudinal survey of the distribution of various serotypes of Streptococcus mutans in infants. J Clin Microbiol 10:497–502
    [Google Scholar]
  21. Newbrun E., Matsukubo T., Hoover C. I., Graves R. C., Brown A. T., Disney A. J., Bohannan H. M. 1984; Comparison of two screening tests for Streptococcus mutans and evaluation of their suitability for mass screenings and private practice. Community Dent Oral Epidemiol 12:325–331 [CrossRef]
    [Google Scholar]
  22. Nie M., Fan M., Bian K. 2002; Transmission of mutans streptococci in adults within a Chinese population. Caries Res 36:161–166 [CrossRef]
    [Google Scholar]
  23. Okada M., Hayashi F., Nagasaka N. 2000; Detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in dental plaque samples from children 2 to 12 years of age. J Clin Periodontol 27:763–768 [CrossRef]
    [Google Scholar]
  24. Okada M., Soda Y., Hayashi F., Doi T., Suzuki J., Miura K., Kozai K. 2002; PCR detection of Streptococcus mutans and S.sobrinus in dental plaque samples from Japanese pre-school children. J Med Microbiol 51:443–447
    [Google Scholar]
  25. Ono T., Hirota K., Nemoto K., Fernandez E. J., Ota F., Fukui K. 1994; Detection of Streptococcus mutans by PCR amplification of spaP gene. J Med Microbiol 41:231–235 [CrossRef]
    [Google Scholar]
  26. Saarela M., Hannula J., Mättö J., Asikainen S., Alaluusua S. 1996; Typing of mutans streptococci by arbitrarily primed polymerase chain reaction. Arch Oral Biol 41:821–826 [CrossRef]
    [Google Scholar]
  27. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Shiroza T., Shinozaki N., Watanabe T., Ikemi T., Fukushima K., Abiko Y. 1998; Rapid isolation of chromosomal DNA from oral streptococci and polymerase chain reaction-oriented restriction fragment-length polymorphism analysis for genetic heterogeneity. Oral Microbiol Immunol 13:11–16 [CrossRef]
    [Google Scholar]
  29. Smith G. L., Socransky S. S., Smith C. M., Scully C., Gilmour G. 1989; Rapid method for the purification of DNA from subgingival microorganisms. Oral Microbiol Immunol 4:47–51 [CrossRef]
    [Google Scholar]
  30. Straetemans M. M. E., van Loveren C., de Soet J. J., de Graaff J., ten Cate J. 1998; Colonization with mutans streptococci and lactobacilli and the caries experience of children after the age of five. J Dent Res 77:1851–1855 [CrossRef]
    [Google Scholar]
  31. Svanberg M., Krasse B. 1990; Comparative recovery of mutans streptococci on two selective media. Caries Res 24:36–38
    [Google Scholar]
  32. Wade W. G., Aldred M. J., Walker D. M. 1986; An improved medium for isolation of Streptococcus mutans . J Med Microbiol 22:319–323 [CrossRef]
    [Google Scholar]
  33. Whiley R. A., Beighton D. 1998; Current classification of the oral streptococci. Oral Microbiol Immunol 13:195–216 [CrossRef]
    [Google Scholar]
  34. WHO 1987; Individual tooth status and treatment need. In Oral Health Surveys: Basic Methods . , 3rd edn. pp 34–39 Geneva: World Health Organization;
  35. Wilson K. 1990; Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology 2.4.1–2.4.2.4.2 Edited by Ausubel F. M. and others Philadelphia: Wiley Interscience;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46069-0
Loading
/content/journal/jmm/10.1099/jmm.0.46069-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error