1887

Abstract

is increasingly recognized as a common and important pathogen in community settings, and is responsible for various pulmonary and extrapulmonary conditions in the normal population. However, the seroepidemiology of acute infection in HIV-infected individuals is still unclear worldwide. This study examined the seroprevalence of antibodies to in HIV-infected patients admitted with respiratory complaints at a tertiary AIDS care centre in Chennai, India. A commercial gelatin microparticle agglutination test (Serodia-Myco II, Fujirebio) was used for the determination of antibodies against in acute serum specimens. Of the 200 HIV-infected patients with underlying pulmonary conditions tested, 34 (17 % positivity; 95 % CI 12–23 %) had antibodies specific to , while among the 40 patients with no underlying pulmonary symptoms, five (12.5 % positivity; 95 % CI 4–27 %) had evidence of anti- antibody. This shows that the incidence of seropositivity is greater in patients with underlying pulmonary complaints. Most positive titres were found in the age group 28–37 years in the symptomatic and symptom-free groups (64.7 and 60 %, respectively). The positive titres ranged from 40 to >20 480. High titres (⩾320) were found in 10 out of the 39 patients (25.6 %). This seroprevalence study reports a 16.2 % prevalence of infections in HIV-infected patients by a particle agglutination test.

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2006-06-01
2024-03-29
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References

  1. Atkinson T., Duffy L. B., Pendley D., Dai Y., Cassell G. H. 2004; Reduced IgG antibody to Mycoplasma pneumoniae a mong subjects with asthma: results of a five-year study. J Allergy Clin Immunol 115:S171
    [Google Scholar]
  2. Blanchard A., Montagnier L. 1994; AIDS-associated mycoplasmas. Annu Rev Microbiol 48:687 [CrossRef]
    [Google Scholar]
  3. Brunner H., Prescott B., Greenberg H., James W. D., Horswood R. L., Chanock R. M. 1977; Unexpectedly high frequency of antibody to Mycoplasma pneumoniae in human sera as measured by sensitive techniques. J Infect Dis 135:524–530 [CrossRef]
    [Google Scholar]
  4. Cassell G. H., Cole B. C. 1981; Mycoplasmas as agents of human disease. N Engl J Med 304:80–89 [CrossRef]
    [Google Scholar]
  5. Chamberlein P., Saeed A. A. 1983; A study of the specific IgM antibody response in Mycoplasma pneumoniae in man. J Hyg Camb 90:207–211 [CrossRef]
    [Google Scholar]
  6. Cimolai N., Cheong A. C. H. 1996; An assessment of a new diagnostic indirect enzyme immunoassay for the detection of anti- Mycoplasma pneumoniae IgM. Am J Clin Pathol 105:205–209
    [Google Scholar]
  7. Clyde W. A. Jr 1983; Mycoplasma pneumoniae respiratory disease symposium: summation and significance. Yale J Biol 56:523–527
    [Google Scholar]
  8. Dey A. B., Chaudhry R., Kumar P., Nisar N., Nagarkar K. M. 2000; Mycoplasma pneumoniae and community-acquired pneumonia. Natl Med J India 13:66–70
    [Google Scholar]
  9. Dussaix E., Slim A., Tournier P. 1983; Comparison of enzyme-linked immunosorbent assay (ELISA) and complement fixation test for detection of Mycoplasma pneumoniae antibodies. J Clin Pathol 36:228–232 [CrossRef]
    [Google Scholar]
  10. Fedorko D. P., Emery D. D., Franklin S. M., Congdon D. D. 1995; Evaluation of a rapid enzyme immunoassay system for serologic diagnosis of Mycoplasma pneumoniae infection. Diagn Microbiol Infect Dis 23:85–88 [CrossRef]
    [Google Scholar]
  11. Gendrel D., Raymond J., Moulin F., Iniguez J. L., Ravilly S., Habib F., Lebon P., Kalifa G. 1997; Etiology and response to antibiotic therapy of community-acquired pneumonia in French children. Eur J Clin Microbiol Infect Dis 16:388–391 [CrossRef]
    [Google Scholar]
  12. Hammerschlag M. R. 2001; Mycoplasma pneumoniae infections. Curr Opin Infect Dis 14:181–186 [CrossRef]
    [Google Scholar]
  13. Ikeda A., Omori S. 2004 Experience with Serodia-Myco in indirect hemagglutination tests for antibody against Mycoplasma pneumoniae . Serodia-Myco bibliography 21 Tokyo: Fujirebio Inc;
    [Google Scholar]
  14. Jacobs E. 1993; Serological diagnosis of Mycoplasma pneumoniae infections: a critical review of current procedures. Clin Infect Dis 17:S79–S82 [CrossRef]
    [Google Scholar]
  15. Jacobs E., Bennewitz A., Bredt W. 1986; Reaction pattern of human anti- Mycoplasma pneumoniae antibodies in enzyme-linked immunosorbent assays and immunoblotting. J Clin Microbiol 23:517–522
    [Google Scholar]
  16. Karppelin M., Hakkarainen K., Kleemola M., Miettinen A. 1993; Comparison of three serological methods for diagnosing Mycoplasma pneumoniae infection. J Clin Pathol 46:1120–1123 [CrossRef]
    [Google Scholar]
  17. Kenny G. E., Kaiser G. G., Cooney M. K., Foy H. M. 1990; Diagnosis of Mycoplasma pneumoniae : sensitivity and specifications of serology with lipid antigen and isolation of the organism on soy peptone medium for identification of infections. J Clin Microbiol 28:2087–2093
    [Google Scholar]
  18. Kleemola M., Kayhty H. 1982; Increase in titer of antibodies to Mycoplasma pneumoniae in patients with purulent meningitis. J Infect Dis 146:284–288 [CrossRef]
    [Google Scholar]
  19. Maniloff J., McElhancy R. N., Finch L. R., Baseman J. B. (editors) 1992 Mycoplasmas: Molecular Biology and Pathogenesis Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Marmion B. P., Williamson J., Worswick D. A., Kok T. W., Harris R. J. 1993; Experience with newer techniques for the laboratory detection of Mycoplasma pneumoniae : Adelaide, 1978–1992. Clin Infect Dis 17:S90–S99 [CrossRef]
    [Google Scholar]
  21. Matas L., Dominguez J., De Ory F., Garcia N., Gali N., Cardona P. J., Hernandez A., Rodrigo C., Ausina V. 1998; Evaluation of Meridian ImmunoCard mycoplasma test for the detection of Mycoplasma pneumoniae -specific IgM in paediatric patients. Scand J Infect Dis 30:289–293 [CrossRef]
    [Google Scholar]
  22. Mundy L. M., Oldach D., Auwaerter P. G., Gaydos C. A., Moore R. D., Bartlett J. G., Quinn T. C. 1998; Implications for macrolide treatment in community-acquired pneumonia. Chest 113:1201–1206 [CrossRef]
    [Google Scholar]
  23. Shankar E. M., Kumarasamy N., Krishnakumar R., Balakrishnan P., Solomon S., Usha A. R. 2005; Recovery rates of Mycoplasma pneumoniae in induced sputum and throat swab specimens of HIV infected patients at an AIDS care centre in Chennai, South India. In Abstracts of the 157th Meeting of the Society for General Microbiology, Keele University, UK abstract 18 Reading, UK: Society for General Microbiology;
    [Google Scholar]
  24. Sherman M. J., Cubic H. A., Inglis J. M. 1993; Mycoplasma pneumoniae infection: early diagnosis by detection of specific IgM by immunofluorescence. Br J Biomed Sci 50:305–308
    [Google Scholar]
  25. Sillis M. 1990; The limitations of IgM assays in the serological diagnosis of Mycoplasma pneumoniae infections. J Med Microbiol 33:253–258 [CrossRef]
    [Google Scholar]
  26. Srifuengfung S., Techachaiwiwat W., Dhiraputra C. 2004; Serological study of Mycoplasma pneumoniae infections. J Med Assoc Thai 87:935–938
    [Google Scholar]
  27. Tully J. G., Rose D. L., Whitcomb R. F., Wenzel R. P. 1979; Enhanced isolation of Mycoplasma pneumoniae from throat washing with a newly modified culture medium. J Infect Dis 139:478–482 [CrossRef]
    [Google Scholar]
  28. Twigg H. L. 3rd, Spain B. A., Soliman D. M., Bowen L. K., Heidler K. M., Wilkes D. S. 1996; Impaired IgG production in the lungs of HIV-infected individuals. Cell Immunol 170:127–133 [CrossRef]
    [Google Scholar]
  29. Velleca W. M., Bird B. R., Forrester F. T. (editors) 1980 Laboratory Diagnosis of Mycoplasma Infections Atlanta, GA: Centres for Disease Control;
    [Google Scholar]
  30. Vikerfors T., Brodin G., Grandien M., Hirschberg L., Krook A., Petterson C. A. 1988; Detection of specific IgM antibodies for the diagnosis of Mycoplasma pneumoniae infections: a clinical evaluation. Scand J Infect Dis 20:601–610 [CrossRef]
    [Google Scholar]
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