1887

Abstract

HZ95 is an enterococcus with high-level resistance to aminoglycosides. Nine genes responsible for high-level aminoglycoside resistance, including (′)-(″)-, (″)-, (″)-, (″)-, (′)-, (′)-, (′)-, (′)- and (′)-, were not detected in HZ95. An 8 kb fragment from unconjugative plasmids of HZ95 was cloned, and expressed gentamicin resistance in DH5. The genetic structures (∼8 kb DNA fragment) containing these aminoglycoside-modifying enzyme genes in HZ95 were determined. The deduced amino acid sequence of the novel (″) allele, (″)-, had 93.7 % amino acid identity with APH(2″)-Id. The (″)- gene was bracketed upstream by an insertion sequence (IS)-like element and downstream by a streptomycin adenylyltransferase gene (). The streptomycin adenylyltransferase encoded by the gene had 80.3 % amino acid identity with the protein encoded by . The plasmid of ∼16 kb could hybridize with a PCR-generated (″)- intragenic probe. The IS-like element had 91 % identity with IS. IS, which commonly acts as a key factor in the dissemination of CTX-M-type -lactamase genes in Gram-negative bacteria, has not been reported in .

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2006-11-01
2024-04-19
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References

  1. Ahmed A. M., Shimamoto T. A. 2004; Plasmid-encoded class 1 integron carrying sat , a putative phosphoserine phosphatase gene and aadA2 from enterotoxigenic Escherichia coli O159 isolated in Japan. FEMS Microbiol Lett 235:243–248 [CrossRef]
    [Google Scholar]
  2. Cao V., Lambert T., Courvalin P. 2002; ColE1-like plasmid pIP843 of Klebsiella pneumoniae encoding extended-spectrum beta-lactamase CTX-M-17. Antimicrob Agents Chemother 46:1212–1217 [CrossRef]
    [Google Scholar]
  3. Chow J. W., Zervos M. J., Lerner S. A., Thal L. A., Donabedian S. M., Jaworski D. D., Tsai S., Shaw K. J., Clewell D. B. 1997; A novel gentamicin resistance gene in Enterococcus . Antimicrob Agents Chemother 41:511–514
    [Google Scholar]
  4. Clark N. C., Olsvik O., Swenson J. M., Spiegel C. A., Tenover F. C. 1999; Detection of a streptomycin/spectinomycin adenylyltransferase gene ( aadA ) in Enterococcus faecalis . Antimicrob Agents Chemother 43:157–160 [CrossRef]
    [Google Scholar]
  5. CLSI 2005 Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard . CLSI document M7–A6, 6th edn. Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  6. Derbise A., Aubert S., Solh N. E. 1997; Mapping the regions carrying three contiguous antibiotic resistance genes aadE, sat4 , and aphA-3 in the genomes of staphylococci. Antimicrob Agents Chemother 41:1024–1032
    [Google Scholar]
  7. Donabedian S. M., Thal L. A., Hershberger E. & 13 other authors; 2003; Molecular characterization of gentamicin-resistant enterococci in the United States: Evidence of spread from animals to humans through food. J Clin Microbiol 41:1109–1113 [CrossRef]
    [Google Scholar]
  8. Eliopoulos G. M., Farber B. F., Murray B. E., Wennersten C., Moellering R. C. 1984; Ribosomal resistance of clinical enterococcal isolates to streptomycin. Antimicrob Agents Chemother 25:398–399 [CrossRef]
    [Google Scholar]
  9. Ferretti J. J., Gilmore K. S., Courvalin P. 1986; Nucleotide sequence analysis of the gene specifying the bifunctional 6′-aminoglycoside acetyltransferase enzyme in Streptococcus faecalis and identification and cloning of gene regions specifying the two activities. J Bacteriol 167:631–638
    [Google Scholar]
  10. Hallgren A., Saeedi B., Nilsson M., Monstein H. J., Isaksson B., Hanberger H., Nilsson L. E. 2003; Genetic relatedness among Enterococcus faecalis with transposon-mediated high-level gentamicin resistance in Swedish intensive care units. J Antimicrob Chemother 52:162–167 [CrossRef]
    [Google Scholar]
  11. Ike Y., Tanimoto K., Tomita H., Takeuchi K., Fujimoto S. 1998; Efficient transfer of the pheromone-independent Enterococcus faecium plasmid pMG1 (Gmr) (65.1 kilobases) to Enterococcus strains during broth mating. J Bacteriol 180:4886–4892
    [Google Scholar]
  12. Kao S. J., You I., Clewell D. B., Donabedian S. M., Zervos M. J., Petrin J., Shaw K. J., Chowl J. W. 2000; Detection of the high-level aminoglycoside resistance gene aph ( 2 ″)- Ib in Enterococcus faecium . Antimicrob Agents Chemother 44:2876–2879 [CrossRef]
    [Google Scholar]
  13. Murray B. E. 1998; Diversity among multidrug-resistant enterococci. Emerg Infect Dis 4:37–47 [CrossRef]
    [Google Scholar]
  14. Perreten V., Schwarz F. V., Teuber M., Levy S. B. 2001; Mdt(A), a new efflux protein conferring multiple antibiotic resistance in Lactococcus lactis and Escherichia coli . Antimicrob Agents Chemother 45:1109–1114 [CrossRef]
    [Google Scholar]
  15. Poirel L., Lartigue M. F., Decousser J. W., Nordmann P. 2005; IS Ecp1B -mediated transposition of bla CTX-M in Escherichia coli . Antimicrob Agents Chemother 49:447–450 [CrossRef]
    [Google Scholar]
  16. Projan S. J., Moghazeh S., Novick R. P. 1988; Nucleotide sequence of pS194, a streptomycin-resistance plasmid from Staphylococcus aureus . Nucleic Acids Res 16:2179–2187 [CrossRef]
    [Google Scholar]
  17. Schaberg D. R., Culver D. H., Gaynes R. P. 1991; Major trends in the microbial etiology of nosocomial infection. Am J Med 91 (Suppl. 3B):72S–75S
    [Google Scholar]
  18. Schwarz F. V., Perreten V., Teuber M. 2001; Sequence of the 50-kb conjugative multiresistance plasmid pRE25 from Enterococcus faecalis RE25. Plasmid 46:170–187 [CrossRef]
    [Google Scholar]
  19. Simjee S., Fraise A. P., Gill M. J. 1999; Plasmid heterogeneity and identification of a Tn 5281 -like element in clinical isolates of high-level gentamicin-resistant Enterococcus faecium isolated in the UK. J Antimicrob Chemother 43:625–635 [CrossRef]
    [Google Scholar]
  20. Tai S. F., Zervos M. J., Clewell D. B., Donabedian S. M., Sahm D. F., Chow J. W. 1998; A new high-level gentamicin resistance gene, aph ( 2 ″)- Id , in Enterococcus spp. Antimicrob Agents Chemother 42:1229–1232
    [Google Scholar]
  21. Wang Q., Xu Y. C., Wang H., Chen M. J., Zhang X. Z., Xu S. Z., Wu J. 1999; Antimicrobial resistant characteristics in enterococci and antibiotics for enterococcal infections. Chinese Journal of Laboratory Medicine 22:154–156 (in Chinese
    [Google Scholar]
  22. Werner G., Hildebrandt B., Witte W. 2001; Aminoglycoside-streptothricin resistance gene cluster aadE-sat4-aphA-3 disseminated among multiresistant isolates of Enterococcus faecium . Antimicrob Agents Chemother 45:3267–3269 [CrossRef]
    [Google Scholar]
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