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Volume 156, Issue 1

VEJ, a novel filamentous phage of able to transduce the cholera toxin genes Free

Abstract

A novel filamentous bacteriophage, designated VEJ, was isolated from strain MO45 of of the O139 serogroup. A molecular characterization of the phage was carried out, which included sequencing of its whole genome, study of the genomic structure, identification of the phage receptor, and determination of the function of some of the genes, such as those encoding the major capsid protein and the single-stranded DNA-binding protein. The genome nucleotide sequence of VEJ, which consists of 6842 bp, revealed that it is organized in modules of functionally related genes in an array that is characteristic of the genus (filamentous phages). VEJ is closely related to other previously described filamentous phages of including VGJ, VSK and fs1. Like these phages, VEJ uses as a cellular receptor the type IV fimbria called the mannose-sensitive haemagglutinin (MSHA). It was also demonstrated that VEJ, like phage VGJ, is able to transmit the genome of phage CTX, and therefore the genes encoding the cholera toxin (CT), horizontally among populations of expressing the MSHA receptor fimbria. This suggests that the variety of phages implicated in the horizontal transmission of the CT genes could be more diverse than formerly thought.

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Keyword(s): CT, cholera toxin , MSHA, mannose-sensitive haemagglutinin , RF, replicative form and TCP, toxin co-regulated pilus
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2010-01-01
2024-04-18
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VEJφ, a novel filamentous phage of Vibrio cholerae able to transduce the cholera toxin genes
Microbiology 156, 108 (2010); https://doi.org/10.1099/mic.0.032235-0
/content/journal/micro/10.1099/mic.0.032235-0
/content/journal/micro/10.1099/mic.0.032235-0
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