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Volume 158, Issue 1

RUT-C30 – thirty years of strain improvement Open Access

Abstract

The hypersecreting mutant RUT-C30 (ATCC 56765) is one of the most widely used strains of filamentous fungi for the production of cellulolytic enzymes and recombinant proteins, and for academic research. The strain was obtained after three rounds of random mutagenesis of the wild-type QM6a in a screening program focused on high cellulase production and catabolite derepression. Whereas RUT-C30 achieves outstanding levels of protein secretion and high cellulolytic activity in comparison to the wild-type QM6a, recombinant protein production has been less successful. Here, we bring together and discuss the results from biochemical-, microscopic-, genomic-, transcriptomic-, glycomic- and proteomic-based research on the RUT-C30 strain published over the last 30 years.

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© 2012 SGM
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2012-01-01
2024-04-18
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Trichoderma reesei RUT-C30 – thirty years of strain improvement
Microbiology 158, 58 (2012); https://doi.org/10.1099/mic.0.054031-0
/content/journal/micro/10.1099/mic.0.054031-0
/content/journal/micro/10.1099/mic.0.054031-0
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