1887

Abstract

The presence of , a gene encoding a known virulence factor of strains responsible for neonatal meningitis in humans, was investigated in the genome of 213 avian pathogenic (APEC) strains and 55 non-pathogenic strains of avian origin. Fifty-three strains were found to be , all of which belonged to the APEC group. The gene is therefore positively linked to the pathogenicity of strains (<0·0001). Analysis of the serogroup of strains revealed a positive association of with serogroups O18, O88 and O2. On the contrary, only 1/59 O78 strains are , indicating a negative association of with this serogroup (<0·0001). The role of in the virulence of the APEC strain BEN 2908 was investigated by constructing an mutant. Challenge assays on 3-week-old chickens showed a reduced virulence for the mutant. Furthermore, the APEC strain BEN 2908 was able to invade brain microvascular epithelial cells, this invasion being significantly reduced upon inactivation of . Altogether, these results suggest a role of in the pathogenicity of some APEC strains and confirm the close relationship between APEC and other human extraintestinal pathogenic isolates.

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2005-04-01
2024-03-28
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