1887

Abstract

To assess the stability of polyomavirus antibodies in serial samples over time and the incidence and age-specific prevalence of polyomavirus infections, we established enzyme immunoassays (EIAs) using purified yeast-expressed virus-like particles (VLPs) containing the VP1 major capsid proteins of JC virus (JCV) and the AS and SB strains of BK virus (BKV). A random subsample of 150 Finnish women who had serum samples taken during the first trimester of pregnancy and had a second pregnancy during a 5 year follow-up period was selected, grouped by age of first pregnancy. The polyomavirus antibody levels were similar in samples taken during the first and second pregnancies (correlation coefficient 0·93 for BKV SB and 0·94 for JCV). Analysis of serum samples from 290 Swedish children aged 1–13 years, grouped by age in 2 year intervals, demonstrated that BKV seropositivity increased rapidly with increasing age of the children, reaching 98 % seroprevalence at 7–9 years of age, followed by a minor decrease. JCV seroprevalence increased only slowly with increasing age and reaching 72 % positivity among mothers >25 years of age. The age-specific seroprevalence of the human polyomaviruses measured using this VLP-based EIA was similar to previous serosurveys by other methods. The stability of the antibodies over time indicates that polyomavirus seropositivity is a valid marker of cumulative virus exposure, and polyoma VLP-based EIAs may therefore be useful for epidemiological studies of these viruses.

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2003-06-01
2024-04-16
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References

  1. af Geijersstam V., Kibur M., Wang Z., Koskela P., Pukkala E., Schiller J., Lehtinen M., Dillner J. 1998; Stability over time of serum antibody levels to human papillomavirus type 16. J Infect Dis 177:1710–1714
    [Google Scholar]
  2. af Geijersstam V., Eklund C., Wang Z., Sapp M., Schiller J. T., Dillner J., Dillner L. 1999; A survey of seroprevalence of human papillomavirus types 16, 18 and 33 among children. Int J Cancer 80:489–493
    [Google Scholar]
  3. An K., Lovgren T. R., Tilley M. B., Consigli R. A. 2000; Use of the confocal microscope to determine polyomavirus recombinant capsid-like particle entry into mouse 3T6 cells. J Virol Methods 84:153–159
    [Google Scholar]
  4. Barbanti-Brodano G., Martini F., De Mattei M., Lazzarin L., Corallini A., Tognon M. 1998; BK and JC human polyomaviruses and simian virus 40: natural history of infection in humans, experimental oncogenicity, and association with human tumors. Adv Virus Res 50:69–99
    [Google Scholar]
  5. Del Valle L., Gordon J., Assimakopoulou M., Enam S., Geddes J. F., Varakis J. N., Katsetos C. D., Croul S., Khalili K. 2001; Detection of JC virus DNA sequences and expression of the viral regulatory protein T-antigen in tumors of the central nervous system. Cancer Res 61:4287–4293
    [Google Scholar]
  6. Dillner J. 1999; The serological response to papillomaviruses. Semin Cancer Biol 9:423–430
    [Google Scholar]
  7. Dorries K. 1998; Molecular biology and pathogenesis of human polyomavirus infections. Dev Biol Stand 94:71–79
    [Google Scholar]
  8. Flaegstad T., Andresen P. A., Johnsen J. I., Asomani S. K., Jorgensen G. E., Vignarajan S., Kjuul A., Kogner P., Traavik T. 1999; A possible contributory role of BK virus infection in neuroblastoma development. Cancer Res 59:1160–1163
    [Google Scholar]
  9. Gedvilaite A., Frommel C., Sasnauskas K. 7 other authors 2000; Formation of immunogenic virus-like particles by inserting epitopes into surface-exposed regions of hamster polyomavirus major capsid protein. Virology 273:21–35
    [Google Scholar]
  10. Hale A. D., Bartkeviciute D., Dargeviciute A., Jin L., Knowles W., Staniulis J., Brown D. W., Sasnauskas K. 2002; Expression and antigenic characterization of the major capsid proteins of human polyomaviruses BK and JC in Saccharomyces cerevisiae . J Virol Methods 104:93–98
    [Google Scholar]
  11. Hamilton R. S., Gravell M., Major E. O. 2000; Comparison of antibody titers determined by hemagglutination inhibition and enzyme immunoassay for JC virus and BK virus. J Clin Microbiol 38:105–109
    [Google Scholar]
  12. Hou J., Major E. O. 2000; Progressive multifocal leukoencephalopathy: JC virus induced demyelination in the immune compromised host. J Neurovirol 6:S98–100
    [Google Scholar]
  13. Jin L., Gibson P. E., Booth J. C., Clewley J. P. 1993a; Genomic typing of BK virus in clinical specimens by direct sequencing of polymerase chain reaction products. J Med Virol 41:11–17
    [Google Scholar]
  14. Jin L., Gibson P. E., Knowles W. A., Clewley J. P. 1993b; BK virus antigenic variants: sequence analysis within the capsid VP1 epitope. J Med Virol 39:50–56
    [Google Scholar]
  15. Kibur M., af Geijerstamm V., Pukkala E. 7 other authors 2000; Attack rates of human papillomavirus type 16 and cervical neoplasia in primiparous women and field trial designs for HPV16 vaccination. Sex Transm Infect 76:13–17
    [Google Scholar]
  16. Knowles W. A., Sharp I. R., Efstratiou L., Hand J. F., Gardner S. D. 1991; Attack rates of human papillomavirus type 16 and cervical neoplasia in primiparous women and field trial designs for HPV16 vaccination. J Med Virol 34:127–131
    [Google Scholar]
  17. Limaye A. P., Jerome K. R., Kuhr C. S., Ferrenberg J., Huang M. L., Davis C. L., Corey L., Marsh C. L. 2001; Quantitation of BK virus load in serum for the diagnosis of BK virus-associated nephropathy in renal transplant recipients. J Infect Dis 183:1669–1672
    [Google Scholar]
  18. Ou W. C., Wang M., Fung C. Y., Tsai R. T., Chao P. C., Hseu T. H., Chang D. 1999; The major capsid protein, VP1, of human JC virus expressed in Escherichia coli is able to self-assemble into a capsid-like particle and deliver exogenous DNA into human kidney cells. J Gen Virol 80:39–46
    [Google Scholar]
  19. Rencic A., Gordon J., Otte J., Curtis M., Kovatich A., Zoltick P., Khalili K., Andrews D. 1996; Detection of JC virus DNA sequence and expression of the viral oncoprotein, tumor antigen, in brain of immunocompetent patient with oligoastrocytoma. Proc Natl Acad Sci U S A 93:7352–7357
    [Google Scholar]
  20. Sasnauskas K., Buzaite O., Vogel F., Jandrig B., Razanskas R., Staniulis J., Scherneck S., Kruger D. H., Ulrich R. 1999; Yeast cells allow high-level expression and formation of polyomavirus-like particles. Biol Chem 380:381–386
    [Google Scholar]
  21. Shah K. V. 1996 Polyomaviruses Philadelphia: Lippincott–Raven;
    [Google Scholar]
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