1887

Abstract

Hepatitis B virus (HBV) genotypes have a characteristic geographical distribution. More than 90 % of chronic HBV patients in China are infected with genotypes B or C. Here, eight HBV isolates that were initially classified as genotype D by PCR-restriction fragment length polymorphism analysis were analysed in detail. The complete HBV genome was sequenced and compared with 32 sequences retrieved from GenBank, representing HBV genotypes A–G. Phylogenetic analysis of the S gene (nt 10–800) classified all eight isolates as genotype D. However, phylogenetic analyses of nt 800–10 and the open reading frames (ORFs) of the precore/core and X genes classified all eight isolates as genotype C. This discordance between phylogenetic trees reconstructed on different ORFs suggested that intertype recombination has occurred in all eight isolates. By using the program, the site of recombination with genotype D was located in the preS2/S region, spanning nt 10–799 in seven of eight isolates and nt 10–1499 in the other isolate. These results demonstrate that intertype recombination should be considered as a type of variation that increases the genetic diversity of HBV. Hybrids of different HBV genotypes might exhibit specific virological properties and their significance in the diagnosis and management of chronic hepatitis B deserves further investigation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.80771-0
2005-04-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/86/4/vir860985.html?itemId=/content/journal/jgv/10.1099/vir.0.80771-0&mimeType=html&fmt=ahah

References

  1. Arauz-Ruiz P., Norder H., Robertson B. H., Magnius L. O. 2002; Genotype H: a new Amerindian genotype of hepatitis B virus revealed in Central America. J Gen Virol 83:2059–2073
    [Google Scholar]
  2. Bollyky P. L., Rambaut A., Harvey P. H., Holmes E. C. 1996; Recombination between sequences of hepatitis B virus from different genotypes. J Mol Evol 42:97–102 [CrossRef]
    [Google Scholar]
  3. Bowyer S. M., Sim J. G. M. 2000; Relationships within and between genotypes of hepatitis B virus at points across the genome: footprints of recombination in certain isolates. J Gen Virol 81:379–392
    [Google Scholar]
  4. Bowyer S. M., van Staden L., Kew M. C., Sim J. G. M. 1997; A unique segment of the hepatitis B virus group A genotype identified in isolates from South Africa. J Gen Virol 78:1719–1729
    [Google Scholar]
  5. Cui C., Shi J., Hui L., Xi H., Zhuoma, Quni Tsedan., Hu G. 2002; The dominant hepatitis B virus genotype identified in Tibet is a C/D hybrid. J Gen Virol 83:2773–2777
    [Google Scholar]
  6. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  7. Günther S., Li B. C., Miska S., Krüger D. H., Meisel H., Will H. 1995; A novel method for efficient amplification of whole hepatitis B virus genomes permits rapid functional analysis and reveals deletion mutants in immunosuppressed patients. J Virol 69:5437–5444
    [Google Scholar]
  8. Hannoun C., Norder H., Lindh M. 2000; An aberrant genotype revealed in recombinant hepatitis B virus strains from Vietnam. J Gen Virol 81:2267–2272
    [Google Scholar]
  9. Kimbi G. C., Kramvis A., Kew M. C. 2004; Distinctive sequence characteristics of subgenotype A1 isolates of hepatitis B virus from South Africa. J Gen Virol 85:1211–1220 [CrossRef]
    [Google Scholar]
  10. Kramvis A., Weitzmann L., Owiredu W. K. B. A., Kew M. C. 2002; Analysis of the complete genome of subgroup A′ hepatitis B virus isolates from South Africa. J Gen Virol 83:835–839
    [Google Scholar]
  11. Lee W. M. 1997; Hepatitis B virus infection. N Engl J Med 337:1733–1745 [CrossRef]
    [Google Scholar]
  12. Lole K. S., Bollinger R. C., Paranjape R. S., Gadkari D., Kulkarni S. S., Novak N. G., Ingersoll R., Sheppard H. W., Ray S. C. 1999; Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. J Virol 73:152–160
    [Google Scholar]
  13. Luo K., Hou J., Liang C. 1996; Hepatitis B virus infection and its genetic variations in China. Chin J Infect Dis 14:101–104
    [Google Scholar]
  14. Luo K., Liu Z., He H., Peng J., Liang W., Dai W., Hou J. 2004; The putative recombination of hepatitis B virus genotype B with pre-C/C region of genotype C. Virus Genes 29:31–41 [CrossRef]
    [Google Scholar]
  15. Miyakawa Y., Mizokami M. 2003; Classifying hepatitis B virus genotypes. Intervirology 46:329–338 [CrossRef]
    [Google Scholar]
  16. Morozov V., Pisareva M., Groudinin M. 2000; Homologous recombination between different genotypes of hepatitis B virus. Gene 260:55–65 [CrossRef]
    [Google Scholar]
  17. Norder H., Couroucé A.-M., Magnius L. O. 1994; Complete genomes, phylogenetic relatedness, and structural proteins of six strains of the hepatitis B virus, four of which represent two new genotypes. Virology 198:489–503 [CrossRef]
    [Google Scholar]
  18. Okamoto H., Tsuda F., Sakugawa H., Sastrosoewignjo R. I., Imai M., Miyakawa Y., Mayumi M. 1988; Typing hepatitis B virus by homology in nucleotide sequence: comparison of surface antigen subtypes. J Gen Virol 69:2575–2583 [CrossRef]
    [Google Scholar]
  19. Owiredu W. K. B. A., Kramvis A., Kew M. C. 2001; Molecular analysis of hepatitis B virus genomes isolated from black African patients with fulminant hepatitis B. J Med Virol 65:485–492 [CrossRef]
    [Google Scholar]
  20. Robertson D. L., Hahn B. H., Sharp P. M. 1995; Recombination in AIDS viruses. J Mol Evol 40:249–259 [CrossRef]
    [Google Scholar]
  21. Stuyver L., De Gendt S., Van Geyt C., Zoulim F., Fried M., Schinazi R. F., Rossau R. 2000; A new genotype of hepatitis B virus: complete genome and phylogenetic relatedness. J Gen Virol 81:67–74
    [Google Scholar]
  22. Sugauchi F., Orito E., Ichida T. & 9 other authors; 2002; Hepatitis B virus of genotype B with or without recombination with genotype C over the precore region plus the core gene. J Virol 76:5985–5992 [CrossRef]
    [Google Scholar]
  23. Sugauchi F., Orito E., Ichida T. & 10 other authors; 2003; Epidemiologic and virologic characteristics of hepatitis B virus genotype B having the recombination with genotype C. Gastroenterology 124:925–932 [CrossRef]
    [Google Scholar]
  24. Sugauchi F., Kumada H., Acharya S. A. & 10 other authors; 2004; Epidemiological and sequence differences between two subtypes (Ae and Aa) of hepatitis B virus genotype A. J Gen Virol 85:811–820 [CrossRef]
    [Google Scholar]
  25. Yan L., Hou J.-L., Guo Y.-B., Chen J.-J., Wang Z.-H., Lin Y.-L., Luo K.-X., Niu Z.-Y. 2001; Determination and analysis of complete nucleotide sequence of Chinese genotype D hepatitis B virus: the first report. Di Yi Jun Yi Da Xue Xue Bao 21:848–851
    [Google Scholar]
  26. Zeng G.-B., Wen S.-J., Wang Z.-H., Yan L., Sun J., Hou J.-L. 2004; A novel hepatitis B virus genotyping system by using restriction fragment length polymorphism patterns of S gene amplicons. World J Gastroenterol 10:3132–3136
    [Google Scholar]
  27. Zeng G., Wang Z., Wen S. & 10 other authors 2005; Geographic distribution, virologic and clinical characteristics of hepatitis B virus genotypes In China. J Viral Hepat (in press)
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.80771-0
Loading
/content/journal/jgv/10.1099/vir.0.80771-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error