1887

Abstract

SUMMARY

The DNA of red cowpox virus strain Brighton or its white pock variant was analysed by cleavage with restriction endonucleases dIII, 1, 1 or 1. Physical maps were constructed and the genomes compared with that of vaccinia virus strain DIE. The size of the red cowpox genome is 23 to 29 megadaltons greater than that of vaccinia and results from the presence of additional, near terminal sequences. An internal region of about 75 megadaltons appears to be highly conserved between the two viruses. The red cowpox genome contains near terminal, repetitive sequences which have some homology with those of vaccinia virus DNA. Rapid renaturation of red cowpox terminal restriction fragments indicates that these are covalently cross-linked.

Viable white pock variants arise continually and map as deletion mutants lacking similar sequences from one specific terminus only of the parental genome. The deletion represents 11 to 12% of the red cowpox DNA and includes the terminal repetition which therefore is not required for replication. The deleted terminus of the white pock variant genome does not appear to be cross-linked.

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1979-10-01
2024-04-28
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References

  1. Baxby D., Rondle C. J. M. 1968; The inhibition of growth of vaccinia and cowpox viruses in RK13 cells. Journal of Hygiene, Cambridge 66:191–205
    [Google Scholar]
  2. De Filippes F. M. 1976; Restriction enzyme digests of rapidly renaturing fragments of vaccinia virus DNA. Journal of Virology 17:227–238
    [Google Scholar]
  3. Downie A. W. 1939; The immunological relationship of the virus of spontaneous cowpox to vaccinia virus. British Journal of Experimental Pathology 20:158–176
    [Google Scholar]
  4. Downie A. W., Haddock D. W. 1952; A variant of cowpox virus. Lancet i:1049–1050
    [Google Scholar]
  5. Fenner F., Burnet F. M. 1957; A short description of the Poxvirus group (vaccinia and related viruses). Virology 4:305–314
    [Google Scholar]
  6. Gangemi J. D., Sharp D. G. 1976; Use of a restriction endonuclease in analysing the genomes from two different strains of vaccinia virus. Journal of Virology 20:319–323
    [Google Scholar]
  7. Gemmell A., Cairns H. 1959; Linkage in the genome of an animal virus. Virology 8:381–383
    [Google Scholar]
  8. Gemmell A., Fenner F. 1960; Genetic studies with mammalian poxviruses. III. White (u) mutants of rabbitpox virus. Virology n:219–235
    [Google Scholar]
  9. Geshelin P., Berns K. L. 1974; Characterization and localization of the naturally occurring cross-links in vaccinia virus DNA. Journal of Molecular Biology 88:785–796
    [Google Scholar]
  10. Jaureguiberry G. 1977; Cleavage of vaccinia virus DNA by restriction endonuclease Bal 1, Eco Ri, Bam Hi. Isolation of the natural cross-links. FEBS Letters 83:111–117
    [Google Scholar]
  11. Jeffreys A. J., Flavell R. A. 1977; A physical map of the DNA regions flanking the rabbit B-globin gene. Cell 12:429–439
    [Google Scholar]
  12. Joklik W. K. 1962; The purification of four strains of poxvirus. Virology 18:9–18
    [Google Scholar]
  13. Mccarron R. J., Cabrera C. V., Estaban M., Mcallister W. T., Holowczak J. A. 1978; Structure of vaccinia DNA: analysis of the viral genome by restriction endonucleases. Virology 86:88–101
    [Google Scholar]
  14. Müller H. K., Wittek R., Schaffner W., Schümperli D., Menna A., Wyler R. 1978; Comparison of five poxvirus genomes by analysis with restriction endonucleases Hin dIII, BamI and Eco R1. Journal of General Virology 38:135–147
    [Google Scholar]
  15. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase 1. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  16. Rondle C. J. M., Dumbell K. R. 1962; Antigens of cowpox virus. Journal of Hygiene, Cambridge 60:41–49
    [Google Scholar]
  17. Sarov L., Becker Y. 1967; Studies on vaccinia virus DNA. Virology 33:369–375
    [Google Scholar]
  18. Schaffner W., Gross K., Telford J., Birnstiel M. 1976; Molecular analysis of the histone gene cluster of Psammechinus miliaris. II. The arrangement of the five histone-coding and spacer sequences. Cell 8:471–478
    [Google Scholar]
  19. Schümperlui D., Lagadec R., Müller H. K. 1978; DNA sequence homology estimation by combinatorial analysis of endonuclease restriction data. Journal of General Virology 38:161–166
    [Google Scholar]
  20. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–518
    [Google Scholar]
  21. Van Tongeren H. A. E. 1952; Spontaneous mutation of a cowpox virus by means of egg passage. Archivfiir die gesamte Virusforschung 5:35–52
    [Google Scholar]
  22. Wittek R., Menna A., Schümperlui D., Stoffel S., Müller H. K., Wyler R. 1977; Hin dIII and Sst I restriction sites mapped on rabbit poxvirus and vaccinia virus DNA. Journal of Virology 23:669–678
    [Google Scholar]
  23. Wittek R., Menna A., Müller H. K., Schümperlui D., Boseley P. G., Wyler R. 1978a; Inverted terminal repeats in rabbit poxvirus and vaccinia virus DNA. Journal of Virology 28:171–181
    [Google Scholar]
  24. Wittek R., Müller H. K., Menna A., Wyler R. 1978b; Length of heterogeneity in the DNA of vaccinia virus is eliminated on cloning the virus. FEBS Letters 90:41–46
    [Google Scholar]
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