1887

Journal of General Virology header logo

Volume 73, Issue 8

Scheme for the generation of a truncated endogenous murine leukaemia virus, the resistance gene Free

Abstract

The resistance () gene is a truncated endogenous murine leukaemia virus (MuLV) containing a 3′ portion of , the entire gene and the 3′ long terminal repeat. Env expression renders mice resistant to infection by ecotropic MuLVs, probably via receptor interference. Previous studies have suggested that the flanking cellular sequences are also important for gene expression. To establish how the truncated retrovirus was generated and the nature of the cellular sequences involved, the susceptible () allele DNA was cloned, and its restriction map and nucleotide sequence were compared with those of the allele. A likely mechanism for generation of the truncated endogenous MuLV is suggested by the results; integration of a prototype MuLV provirus at a site within the allele about 6 to 8 kb downstream of a non-retroviral promoter region, followed by deletion of the 5′ half of the provirus, with an accompanying loss of only 7 or 10 bp of cellular flanking sequences. The deletion may have led to the expression of the gene under control of the non-retroviral promoter.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1992
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-8-1925
1992-08-01
2024-05-01
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/8/JV0730081925.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-8-1925&mimeType=html&fmt=ahah

References

  1. Albritton L. M., Tseng L., Scadden D., Cunningham J. M. 1989; A putative murine ecotropic retrovirus receptor gene encodes a multiple membrane-spanning protein and confers susceptibility to virus infection. Cell 57:659–666
     [Google Scholar]
  2. Craigie R., Fujiwara T., Bushman F. 1990; The IN protein of Moloney murine leukemia virus processes the viral DNA ends and accomplishes their integration in vitro . Cell 62:829–837
     [Google Scholar]
  3. Dhar R., McClements W. L., Enquist L. W., Van De Woude G. F. 1980; Nucleotide sequences of integrated Moloney sarcoma provirus long terminal repeats and their host and viral junctions. Proceedings of the National Academy of Sciences, U.S.A 77:3937–3941
     [Google Scholar]
  4. Gorman C. M., Moffat L. F., Howard B. H. 1982; Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Molecular and Cellular Biology 2:1044–1051
     [Google Scholar]
  5. Hartley J. W., Rowe W. P. 1976; Naturally occurring murine leukemia viruses in wild mice: characterization of a new ‘amphotropic’ class. Journal of Virology 19:19–25
     [Google Scholar]
  6. Herr W., Corbin V., Gilbert W. 1982; Nucleotide sequence of the 3′ half of AKV. Nucleic Acids Research 10:6931–6944
     [Google Scholar]
  7. Ikeda H., Odaka T. 1983; Cellular expression of murine leukemia virus gp70-related antigen on thymocytes of uninfected mice correlates with Fv-4 gene-controlled resistance to Friend leukemia virus infection. Virology 128:127–139
     [Google Scholar]
  8. Ikeda H., Odaka T. 1984; A cell membrane ‘gp70’ associated with Fv-4 gene: immunological characterization, and tissue and strain distribution. Virology 133:65–76
     [Google Scholar]
  9. Ikeda H., Sugimura H. 1989; Fv-4 resistance gene: a truncated endogenous murine leukemia virus with ecotropic interference properties. Journal of Virology 63:5405–5412
     [Google Scholar]
  10. Ikeda H., Sato H., Odaka T. 1981; Mapping of the Fv-4 gene controlling resistance to murine leukemia viruses. International Journal of Cancer 28:237–240
     [Google Scholar]
  11. Ikeda H., Laigret F., Martin M. A., Repaske R. 1985; Characterization of a molecularly cloned retroviral sequence associated with Fv-4 resistance. Journal of Virology 55:768–777
     [Google Scholar]
  12. Inaguma Y., Miyashita N., Moriwaki K., Hiai W. C., Mei-Lei J., Xinqiao H., Ikeda H. 1991; Acquisition of two endogenous ecotropic murine leukemia viruses in distinct Asian wild mouse populations. Journal of Virology 65:1796–1802
     [Google Scholar]
  13. Kai K., Ikeda H., Yuasa Y., Suzuki S., Odaka T. 1976; Mouse strain resistant to N-, B-, and NB-tropic murine leukemia viruses. Journal of Virology 20:436–440
     [Google Scholar]
  14. Kozak C. A., O’Neill R. R. 1987; Diverse wild mouse origins of xenotropic, mink cell focus-forming, and two types of ecotropic proviral genes. Journal of Virology 61:3082–3088
     [Google Scholar]
  15. Masuda M., Yoshikura H. 1990; Construction and characterization of recombinant Moloney murine leukemia viruses bearing the mouse Fv-4 env gene. Journal of Virology 64:1033–1043
     [Google Scholar]
  16. Odaka T., Ikeda H., Moriwaki K., Matsuzawa A., Mizuno M., Kondo K. 1978; Genetic resistance in Japanese wild mice (Mus musculus molossinus) to an NB-tropic Friend murine leukemia virus. Journal of the National Cancer Institute 67:1123–1127
     [Google Scholar]
  17. Odaka T., Ikeda H., Yoshikura H., Moriwaki K., Suzuki S. 1981; Fv-4: gene controlling resistance to NB-tropic Friend murine leukemia virus. Distribution in wild mice, introduction into genetic background of BALB/c mice, and mapping of chromosomes. Journal of the National Cancer Institute 67:1123–1127
     [Google Scholar]
  18. Okayama H., Berg P. 1983; A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Molecular and Cellular Biology 3:280–289
     [Google Scholar]
  19. Oliff A., Gordon I., Hager L., Chang E. H., Scolnick E. M., Chan H. W., Lowy D. R. 1980; Transfection of molecularly cloned Friend murine leukemia virus DNA yields a highly leukemogenic helper-independent type C virus. Journal of Virology 33:475–486
     [Google Scholar]
  20. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
     [Google Scholar]
  21. Shinnick T. M., Lerner R. A., Sutcliffe J. G. 1981; Nucleotide sequence of Moloney murine leukemia virus. Nature, London 293:543–548
     [Google Scholar]
  22. Southern P. J., Berg P. 1982; Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. Journal of Molecular and Applied Genetics 1:327–341
     [Google Scholar]
  23. Suzuki S. 1975; Fv-4: a new gene affecting splenomegaly induction by Friend leukemia virus. Japanese Journal of Experimental Medicine 45:473–478
     [Google Scholar]
  24. Yamaguchi M., Hayashi Y., Matsukage A. 1989; Mouse DNA polymerase beta gene promoter: fine mapping and involvement of Spl-like mouse transcription factor in its function. Nucleic Acids Research 16:8773–8787
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-8-1925
Loading
Scheme for the generation of a truncated endogenous murine leukaemia virus, the Fv-4 resistance gene
J. Gen. Virol. 73, 1925 (1992); https://doi.org/10.1099/0022-1317-73-8-1925
/content/journal/jgv/10.1099/0022-1317-73-8-1925
/content/journal/jgv/10.1099/0022-1317-73-8-1925
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error