1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 57, Issue 5

gen. nov., sp. nov., a mesophilic marine bacterium of the family , isolated from the rhizosphere of Free

Abstract

A marine bacterium, DOKDO 007, was isolated from the rhizosphere of the marine alga collected from Dokdo Island, Korea, in October 2004. The strain produced orange-coloured colonies on marine agar 2216. 16S rRNA gene sequence analysis indicated that the novel isolate belonged to the family and showed relatively high sequence similarities with members of the genus (92.0–94.0 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genera and . Cells were aerobic, Gram-negative rods producing non-diffusible carotenoid pigments. In contrast to all other members of the family , cells of DOKDO 007 were motile by means of a polar flagellum. Optimal growth occurred in the presence of 3.5–4 % (w/v) sea salts (corresponding to 2.7–3.1 % NaCl), at pH 8 and at temperatures of 26–29 °C. The novel strain required Ca ions in addition to NaCl for growth. The dominant fatty acids were iso-15 : 0, iso-15 : 110 and 10-methyl-16 : 0. The major respiratory quinone was MK-6. The DNA G+C content was 56.3 mol%, an unusually high value for members of the family . On the basis of these polyphasic taxonomic data, strain DOKDO 007 should be classified as representing a new genus and novel species in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is DOKDO 007 (=KCCM 42307=JCM 13831).

  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64565-0
2007-05-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/5/1050.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64565-0&mimeType=html&fmt=ahah

References

  1. Bae S. S., Lee J.-H., Kim S.-J. 2005; Bacillus alveayuensis sp. nov., a thermophilic bacterium isolated from deep-sea sediments of the Ayu Trough. Int J Syst Evol Microbiol 55:1211–1215 [CrossRef]
     [Google Scholar]
  2. Barbeyron T., L'Haridon S., Corre E., Kloareg B., Potin P. 2001 Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [ Cytophaga ] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov. Int J Syst Evol Microbiol 51, 985–997 [CrossRef]
  3. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [CrossRef]
     [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
     [Google Scholar]
  5. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868
     [Google Scholar]
  6. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 511997–2006 [CrossRef]
     [Google Scholar]
  7. Cho J. C., Giovannoni S. J. 2004; Robiginitalea biformata gen. nov., sp. nov., a novel marine bacterium in the family Flavobacteriaceae with a higher G+C content. Int J Syst Evol Microbiol 54:1101–1106 [CrossRef]
     [Google Scholar]
  8. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp  267–287 Edited by Goodfellow M., Minikin D. E. London: Academic Press;
     [Google Scholar]
  9. Fautz E., Reichenbach H. 1980; A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91 [CrossRef]
     [Google Scholar]
  10. Gosink J. J., Woese C. R., Staley J. T. 1998; Polaribacter gen. nov., with three new species, P.irgensii sp. nov., P. franzmannii sp.nov. and P. filamentus sp. nov.,gas vacuolate polar marine bacteria of the Cytophaga-Flavobacterium-Bacteroides group and reclassification of ‘ Flectobacillus glomeratus ’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48:223–235 [CrossRef]
     [Google Scholar]
  11. Ivanova E. P., Nedashkovskaya O. I., Chun J., Lysenko A. M., Frolova G. M., Svetashev V. I., Vysotskii M. V., Mikhailov V. V., Huq A., Colwell R. R. 2001; Arenibacter gen. nov., new genus of the family Flavobacteriaceae and description of a new species, Arenibacter latericius sp. nov. Int J Syst Evol Microbiol 51:1987–1995 [CrossRef]
     [Google Scholar]
  12. Kwon K. K., Lee Y. K., Lee H. K. 2006; Costertonia aggregata gen. nov., sp. nov., a mesophilic marine bacterium of the family Flavobacteriaceae , isolated from a mature biofilm. Int J Syst Evol Microbiol 561349–1353 [CrossRef]
     [Google Scholar]
  13. Nedashkovskaya O. I., Kim S. B., Han S. K., Lysenko A. M., Rohde M., Zhukova N. V., Falsen E., Frolova G. M., Mikhailov V. V., Bae K. S. 2003a Mesonia algae gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi (Kutz) Kornm. Int J Syst Evol Microbiol 53, 1967–1971 [CrossRef]
  14. Nedashkovskaya O. I., Suzuki M., Vysotskii M. V., Mikhailov V. V. 2003b; Arenibacter troitsensis sp. nov., isolated from marine bottom sediment. Int J Syst Evol Microbiol 53:1287–1290 [CrossRef]
     [Google Scholar]
  15. Nedashkovskaya O. I., Kim S. B., Han S. K., Lysenko A. M., Mikhailov V. V., Bae K. S. 2004a; Arenibacter certesii sp. nov., a novel marine bacterium isolated from the green alga Ulva fenestrata . Int J Syst Evol Microbiol 54:1173–1176 [CrossRef]
     [Google Scholar]
  16. Nedashkovskaya O. I., Kim S. B., Han S. K., Lysenko A. M., Rohde M., Rhee M.-S., Frolova G. M., Falsen E., Mikhailov V. V., Bae K. S. 2004b; Maribacter gen. nov., a new member of the family Flavobacteriaceae , isolated from marine habitats, containing the species Maribacter sedimenticola sp.nov., Maribacter aquivivus sp. nov., Maribacter orientalis sp. nov. and Maribacter ulvicola sp. nov.. Int J Syst Evol Microbiol 54:1017–1023 [CrossRef]
     [Google Scholar]
  17. Nedashkovskaya O. I., Suzuki M., Vancanneyt M., Cleenwerck I., Lysenko A. M., Mikhailov V. V., Swings J. 2004c Zobellia amurskyensis sp. nov., Zobellia laminariae sp. nov. and Zobellia russellii sp. nov., novel marine bacteria of the family Flavobacteriaceae . Int J Syst Evol Microbiol 54, 1643–1648 [CrossRef]
  18. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology . pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood R. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  19. Sohn J. H., Kwon K. K., Kang J.-H., Jung H.-B., Kim S.-J. 2004; Novosphingobium pentaromativorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. Int J Syst Evol Microbiol 54:1483–1487 [CrossRef]
     [Google Scholar]
  20. Stackebrandt E., Liesack W. 1993; Nucleic acids and classification. In Handbook of New Bacterial Systematics pp  158–160 Edited by Goodfellow M., O'Donnell A. G. London: Academic Press;
     [Google Scholar]
  21. Yoon J.-H., Kang S.-J., Lee S.-Y., Oh T.-K. 2005a; Maribacter dokdonensis sp. nov., isolated from sea water off a Korean island, Dokdo. Int J Syst Evol Microbiol 55:2051–2055 [CrossRef]
     [Google Scholar]
  22. Yoon J.-H., Lee M.-H., Oh T.-K., Park Y.-H. 2005b; Muricauda flavescens sp. nov. and Muricauda aquimarina sp. nov., isolated from a salt lake near Hwajinpo Beach of the East Sea in Korea, and emended description of the genus Muricauda . Int J Syst Evol Microbiol 55:1015–1019 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64565-0
Loading
Flagellimonas eckloniae gen. nov., sp. nov., a mesophilic marine bacterium of the family Flavobacteriaceae, isolated from the rhizosphere of Ecklonia kurome
Int J Syst Evol Microbiol 57, 1050 (2007); https://doi.org/10.1099/ijs.0.64565-0
/content/journal/ijsem/10.1099/ijs.0.64565-0
/content/journal/ijsem/10.1099/ijs.0.64565-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error