1887

Abstract

A Gram-positive, rod-shaped acid-fast bacterium was isolated from a patient with a post-traumatic chronic skin abscess associated with osteitis. Morphological analysis, 16S rRNA, , and gene sequence analysis, cell-wall fatty acid and mycolic acid composition analyses and biochemical tests showed that the isolate, designated ABO-M06, belonged to the genus . Its phenotype was unique and genetic and phylogenetic findings suggest that strain ABO-M06 represents a novel species within the group. The name sp. nov. is proposed for this novel species, with the type strain ABO-M06 (=CIP 109395=DSM 45070).

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2008-02-01
2024-04-24
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References

  1. Adékambi, T. & Drancourt, M.(2004). Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA and rpoB gene sequencing. Int J Syst Evol Microbiol 54, 2095–2105.[CrossRef] [Google Scholar]
  2. Adékambi, T., Colson, P. & Drancourt, M.(2003).rpoB-based identification of nonpigmented and late-pigmented rapidly growing mycobacteria. J Clin Microbiol 41, 5699–5708.[CrossRef] [Google Scholar]
  3. Adékambi, T., Stein, A., Carvajal, J., Raoult, D. & Drancourt, M.(2006). Description of Mycobacterium conceptionense sp. nov., a Mycobacterium fortuitum group organism isolated from a posttraumatic osteitis inflammation. J Clin Microbiol 44, 1268–1273.[CrossRef] [Google Scholar]
  4. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J.(1997). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef] [Google Scholar]
  5. Brown, B. A., Springer, B., Steingrube, V. A. & other authors(1999).Mycobacterium wolinskyi sp. nov. and Mycobacterium goodii sp. nov., two new rapidly growing species related to Mycobacterium smegmatis and associated with human wound infection: a cooperative study from the International Working Group on mycobacterial taxonomy. Int J Syst Evol Microbiol 49, 1493–1511. [Google Scholar]
  6. Brown-Elliott, B. A. & Wallace, R. J., Jr(2002). Clinical and taxonomic status of pathogenic nonpigmented or late-pigmented rapidly growing mycobacteria. Clin Microbiol Rev 15, 716–746.[CrossRef] [Google Scholar]
  7. Brown-Elliott, B. A. & Wallace, R. J., Jr(2005). Infections caused by nontuberculous Mycobacteria. In Principles and Practice of Infectious Diseases, 6th edn, pp. 2909–2916. Edited by G. L. Mandell, J. E. Bennett & R. Dolin. Philadelphia: Elsevier.
  8. Butler, W. R. & Guthertz, L. S.(2001). Mycolic acid analysis by high performance liquid chromatography for identification of Mycobacterium species. Clin Microbiol Rev 14, 704–726.[CrossRef] [Google Scholar]
  9. Butler, W. R. & Kilburn, J. O.(1990). High performance liquid chromatography patterns of mycolic acids as criteria for identification of Mycobacterium chelonae, Mycobacterium fortuitum and Mycobacterium smegmatis. J Clin Microbiol 28, 2094–2098. [Google Scholar]
  10. Butler, W. R., Thibert, L. & Kilburn, J. O.(1992). Identification of Mycobacterium avium complex strains and some similar species by high performance liquid chromatography. J Clin Microbiol 30, 2698–2704. [Google Scholar]
  11. Devulder, G., Perrière, G., Baty, F. & Flandrois, J. P.(2003).bibi, a bioinformatics bacterial identification tool. J Clin Microbiol 41, 1785–1787.[CrossRef] [Google Scholar]
  12. Devulder, G., Pérouse de Montclos, M. & Flandrois, J. P.(2005). A multigene approach to phylogenetic analysis using the genus Mycobacterium as a model. Int J Syst Evol Microbiol 55, 293–302.[CrossRef] [Google Scholar]
  13. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  14. Galtier, N., Gouy, M. & Gautier, C.(1996).seaview and phylo_win, two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12, 543–548. [Google Scholar]
  15. Kluge, A. G. & Farris, F. S.(1969). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef] [Google Scholar]
  16. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  17. NCCLS(2002). Performance standards for antimicrobial susceptibility testing. Twelfth informational supplement. M100–S12. Wayne, PA: NCCLS.
  18. NCCLS(2003). Susceptibility testing of Mycobacteria, Nocardia, and other aerobic actinomycetes. Approved standard M24-A. Wayne, PA: NCCLS.
  19. Ringuet, H., Akoua-Koffi, C., Honore, S., Varnerot, A., Vincent, V., Berche, P., Gaillard, J. L. & Pierre-Audigier, C.(1999).hsp65 sequencing for identification of rapidly growing mycobacteria. J Clin Microbiol 37, 852–857. [Google Scholar]
  20. Rodríguez-Nava, V., Couble, A., Molinard, C., Sandoval, H., Boiron, P. & Laurent, F.(2004).Nocardia mexicana sp. nov., a new pathogen isolated from human mycetomas. J Clin Microbiol 42, 4530–4535.[CrossRef] [Google Scholar]
  21. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  22. Schinsky, M. F., McNeil, M. M., Whitney, A. M., Steigerwalt, A. G., Lasker, B. A., Floyd, M. M., Hogg, G. G., Brenner, D. J. & Brown, J. M.(2000).Mycobacterium septicum sp. nov., a new rapidly growing species associated with catheter-related bacteraemia. Int J Syst Evol Microbiol 50, 575–581.[CrossRef] [Google Scholar]
  23. Schinsky, M. F., Morey, R. E., Steigerwalt, A. G. & other authors(2004). Taxonomic variation in the Mycobacterium fortuitum third biovariant complex: description of Mycobacterium boenickei sp. nov., Mycobacterium houstonense sp. nov., Mycobacterium neworleansense sp. nov. and Mycobacterium brisbanense sp.nov. and recognition of Mycobacterium porcinum from human clinical isolates. Int J Sys Evol Microbiol 54, 1653–1667.[CrossRef] [Google Scholar]
  24. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  25. Vincent, V., Brown-Elliott, B. A., Jost, K. C., Jr & Wallace, R. J., Jr(2003).Mycobacterium: phenotypic and genotypic identification. In Manual of Clinical Microbiology, 8th edn, pp. 560–584. Edited by P. M. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller & R. H. Yolken. Washington, DC: American Society for Microbiology.
  26. Wallace, R. J., Jr, Brown, B. A., Silcox, V. A., Tsukamura, M., Nash, D. R., Steele, L. C., Steingrube, V. A., Smith, J., Sumter, G. & other authors(1991). Clinical disease, drug susceptibility, and biochemical patterns of the unnamed third biovariant complex of Mycobacterium fortuitum. J Infect Dis 163, 598–603.[CrossRef] [Google Scholar]
  27. Wallace, R. J., Jr, Brown-Elliott, B. A., Hall, L., Roberts, G., Wilson, R. W., Mann, , Crist, C. J., Chiu, S. H., Dunlap, R. & other authors(2002). Clinical and laboratory features of Mycobacteriummageritense. J Clin Microbiol 40, 2930–2935.[CrossRef] [Google Scholar]
  28. Zolg, J. W. & Philippi-Schulz, S.(1994). The superoxide dismutase gene, a target for detection and identification of mycobacteria by PCR. J Clin Microbiol 32, 2801–2812. [Google Scholar]
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Supplements

vol. , part 2, pp. 486–490

HPLC profile of sp. nov. ABO-M06 compared with that of CIP 104534 , CIP 104941 and ATCC 49403 .

Phylogenetic tree based on a partial gene sequence, showing the relationship of ABO-M06 with the 14 most closely related species.

Phylogenetic tree based on a partial gene sequence, showing the relationship of ABO-M06 with the 14 most closely related species.

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