1887

Journal of Medical Microbiology logo

Volume 63, Issue 9

Virulence genes of in the Dominican Republic Free

Abstract

Although the incidence of gastric cancer in the Dominican Republic is not high, the disease remains a significant health problem. We first conducted a detailed analysis of status in the Dominican Republic. In total, 158 patients (103 females and 55 males; mean age 47.1±16.2 years) were recruited. The status of infection was determined based on four tests: rapid urease test, culture test, histological test and immunohistochemistry. The status of and genotypes in was examined using PCR and gene sequencing. The overall prevalence of infection was 58.9 %. No relationship was found between the infection rate and the age range of 17–91 years. Even in the youngest group (patients aged <29 years), the infection rate was 62.5 %. Peptic ulcer was found in 23 patients and gastric cancer was found in one patient. The infection rate in patients with peptic ulcer was significantly higher than that in patients with gastritis (82.6 versus 54.5 %, <0.01). The -positive/ s1m1 genotype was the most prevalent (43/64, 67.2 %). Compared with -negative patients, -positive patients showed more severe gastritis. Furthermore, the presence of was related to the presence of more severe gastritis. All CagA-positive strains had Western-type CagA. In conclusion, we found that infection is a risk factor for peptic ulcer in the Dominican Republic. Patients with -positive could be at higher risk for severe inflammation and atrophy.

  • Received:
  • Accepted:
  • Published Online:
© 2014 The Authors
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.075275-0
2014-09-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jmm/63/9/1189.html?itemId=/content/journal/jmm/10.1099/jmm.0.075275-0&mimeType=html&fmt=ahah

References

  1. Aoki K., Kihaile P. E., Castro M., Disla M., Nyambo T. B., Misumi J. 2004; Seroprevalences of Helicobacter pylori infection and chronic zatrophic gastritis in the United Republic of Tanzania and the Dominican Republic. Environ Health Prev Med 9:170–175 [View Article][PubMed]
     [Google Scholar]
  2. Aoki K., Kihaile P. E., Wenyuan Z., Xianghang Z., Castro M., Disla M., Nyambo T. B., Misumi J. 2005; Comparison of prevalence of chronic atrophic gastritis in Japan, China, Tanzania, and the Dominican Republic. Ann Epidemiol 15:598–606 [View Article][PubMed]
     [Google Scholar]
  3. Argent R. H., Kidd M., Owen R. J., Thomas R. J., Limb M. C., Atherton J. C. 2004; Determinants and consequences of different levels of CagA phosphorylation for clinical isolates of Helicobacter pylori . Gastroenterology 127:514–523 [View Article][PubMed]
     [Google Scholar]
  4. Asaka M., Kimura T., Kudo M., Takeda H., Mitani S., Miyazaki T., Miki K., Graham D. Y. 1992; Relationship of Helicobacter pylori to serum pepsinogens in an asymptomatic Japanese population. Gastroenterology 102:760–766[PubMed]
     [Google Scholar]
  5. Asaka M., Kato M., Takahashi S., Fukuda Y., Sugiyama T., Ota H., Uemura N., Murakami K., Satoh K., Sugano K. Japanese Society for Helicobacter Research 2010; Guidelines for the management of Helicobacter pylori infection in Japan: 2009 revised edition. Helicobacter 15:1–20 [View Article][PubMed]
     [Google Scholar]
  6. Atherton J. C., Cao P., Peek R. M. Jr, Tummuru M. K., Blaser M. J., Cover T. L. 1995; Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. Association of specific vacA types with cytotoxin production and peptic ulceration. J Biol Chem 270:17771–17777 [View Article][PubMed]
     [Google Scholar]
  7. Azevedo N. F., Huntington J., Goodman K. J. 2009; The epidemiology of Helicobacter pylori and public health implications. Helicobacter 14:Suppl 11–7 [View Article][PubMed]
     [Google Scholar]
  8. Azuma T., Yamakawa A., Yamazaki S., Fukuta K., Ohtani M., Ito Y., Dojo M., Yamazaki Y., Kuriyama M. 2002; Correlation between variation of the 3′ region of the cagA gene in Helicobacter pylori and disease outcome in Japan. J Infect Dis 186:1621–1630 [View Article][PubMed]
     [Google Scholar]
  9. Bornschein J., Selgrad M., Wex T., Kuester D., Malfertheiner P. 2012; Serological assessment of gastric mucosal atrophy in gastric cancer. BMC Gastroenterol 12:10 [View Article][PubMed]
     [Google Scholar]
  10. Brenner H., Rothenbacher D., Weck M. N. 2007; Epidemiologic findings on serologically defined chronic atrophic gastritis strongly depend on the choice of the cutoff-value. Int J Cancer 121:2782–2786 [View Article][PubMed]
     [Google Scholar]
  11. Burucoa C., Delchier J. C., Courillon-Mallet A., de Korwin J. D., Mégraud F., Zerbib F., Raymond J., Fauchère J. L. 2013; Comparative evaluation of 29 commercial Helicobacter pylori serological kits. Helicobacter 18:169–179 [View Article][PubMed]
     [Google Scholar]
  12. Calvet X., Ramírez Lázaro M. J., Lehours P., Mégraud F. 2013; Diagnosis and epidemiology of Helicobacter pylori infection. Helicobacter 18:Suppl 15–11 [View Article][PubMed]
     [Google Scholar]
  13. Covacci A., Censini S., Bugnoli M., Petracca R., Burroni D., Macchia G., Massone A., Papini E., Xiang Z. other authors 1993; Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc Natl Acad Sci U S A 90:5791–5795 [View Article][PubMed]
     [Google Scholar]
  14. Cover T. L., Blaser M. J. 1992; Purification and characterization of the vacuolating toxin from Helicobacter pylori . J Biol Chem 267:10570–10575[PubMed]
     [Google Scholar]
  15. Dattoli V. C., Veiga R. V., da Cunha S. S., Pontes-de-Carvalho L. C., Barreto M. L., Alcântara-Neves N. M. 2010; Seroprevalence and potential risk factors for Helicobacter pylori infection in Brazilian children. Helicobacter 15:273–278 [View Article][PubMed]
     [Google Scholar]
  16. Dixon M. F., Genta R. M., Yardley J. H., Correa P. 1996; Classification and grading of gastritis: the updated Sydney System. Am J Surg Pathol 20:1161–1181 [View Article][PubMed]
     [Google Scholar]
  17. Ferlay J., Shin H. R., Bray F., Forman D., Mathers C., Parkin D. M. 2010; Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer 127:2893–2917 [View Article][PubMed]
     [Google Scholar]
  18. Goh K. L., Chan W. K., Shiota S., Yamaoka Y. 2011; Epidemiology of Helicobacter pylori infection and public health implications. Helicobacter 16:Suppl 11–9 [View Article][PubMed]
     [Google Scholar]
  19. Hisada M., Lee M. G., Hanchard B., Owens M., Song Q., van Doorn L. J., Cutler A. F., Gold B. D. 2001; Characteristics of Helicobacter pylori infection in Jamaican adults with gastrointestinal symptoms. J Clin Microbiol 39:212–216 [View Article][PubMed]
     [Google Scholar]
  20. Huang J. Q., Sridhar S., Hunt R. H. 2002; Role of Helicobacter pylori infection and non-steroidal anti-inflammatory drugs in peptic-ulcer disease: a meta-analysis. Lancet 359:14–22 [View Article][PubMed]
     [Google Scholar]
  21. Kodama M., Murakami K., Okimoto T., Abe T., Nakagawa Y., Mizukami K., Uchida M., Inoue K., Fujioka T. 2012a; Helicobacter pylori eradication improves gastric atrophy and intestinal metaplasia in long-term observation. Digestion 85:126–130 [View Article][PubMed]
     [Google Scholar]
  22. Kodama M., Murakami K., Okimoto T., Sato R., Uchida M., Abe T., Shiota S., Nakagawa Y., Mizukami K., Fujioka T. 2012b; Ten-year prospective follow-up of histological changes at five points on the gastric mucosa as recommended by the updated Sydney system after Helicobacter pylori eradication. J Gastroenterol 47:394–403 [View Article][PubMed]
     [Google Scholar]
  23. Leja M., Cine E., Rudzite D., Vilkoite I., Huttunen T., Daugule I., Rumba-Rozenfelde I., Pimanov S., Liepniece-Karele I. other authors 2012; Prevalence of Helicobacter pylori infection and atrophic gastritis in Latvia. Eur J Gastroenterol Hepatol 24:1410–1417 [View Article][PubMed]
     [Google Scholar]
  24. Leung W. K., Wu M. S., Kakugawa Y., Kim J. J., Yeoh K. G., Goh K. L., Wu K. C., Wu D. C., Sollano J. other authors 2008; Screening for gastric cancer in Asia: current evidence and practice. Lancet Oncol 9:279–287 [View Article][PubMed]
     [Google Scholar]
  25. Malaty H. M. 2007; Epidemiology of Helicobacter pylori infection. Best Pract Res Clin Gastroenterol 21:205–214 [View Article][PubMed]
     [Google Scholar]
  26. Malfertheiner P., Megraud F., O’Morain C. A., Atherton J., Axon A. T., Bazzoli F., Gensini G. F., Gisbert J. P., Graham D. Y. other authors 2012; Management of Helicobacter pylori infection – the Maastricht IV/Florence Consensus Report. Gut 61:646–664 [View Article][PubMed]
     [Google Scholar]
  27. Matsuda M., Shiota S., Matsunari O., Watada M., Murakami K., Fujioka T., Yamaoka Y. 2011; Prevalence of two homologous genes encoding glycosyltransferases of Helicobacter pylori in the United States and Japan. J Gastroenterol Hepatol 26:1451–1456[PubMed] [CrossRef]
     [Google Scholar]
  28. Matsunari O., Shiota S., Suzuki R., Watada M., Kinjo N., Murakami K., Fujioka T., Kinjo F., Yamaoka Y. 2012; Association between Helicobacter pylori virulence factors and gastroduodenal diseases in Okinawa, Japan. J Clin Microbiol 50:876–883 [View Article][PubMed]
     [Google Scholar]
  29. Mégraud F. 1997; A growing demand for Helicobacter pylori culture in the near future?. Ital J Gastroenterol Hepatol 29:574–576[PubMed]
     [Google Scholar]
  30. Mégraud F., Lehours P. 2007; Helicobacter pylori detection and antimicrobial susceptibility testing. Clin Microbiol Rev 20:280–322 [View Article][PubMed]
     [Google Scholar]
  31. Miki K. 2011; Gastric cancer screening by combined assay for serum anti-Helicobacter pylori IgG antibody and serum pepsinogen levels – “ABC method”. Proc Jpn Acad, Ser B, Phys Biol Sci 87:405–414 [View Article][PubMed]
     [Google Scholar]
  32. Papatheodoridis G. V., Sougioultzis S., Archimandritis A. J. 2006; Effects of Helicobacter pylori and nonsteroidal anti-inflammatory drugs on peptic ulcer disease: a systematic review. Clin Gastroenterol Hepatol 4:130–142 [View Article][PubMed]
     [Google Scholar]
  33. Parsonnet J., Friedman G. D., Vandersteen D. P., Chang Y., Vogelman J. H., Orentreich N., Sibley R. K. 1991; Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med 325:1127–1131 [View Article][PubMed]
     [Google Scholar]
  34. Peek R. M. Jr, Blaser M. J. 2002; Helicobacter pylori and gastrointestinal tract adenocarcinomas. Nat Rev Cancer 2:28–37 [View Article][PubMed]
     [Google Scholar]
  35. Porras C., Nodora J., Sexton R., Ferreccio C., Jimenez S., Dominguez R. L., Cook P., Anderson G., Morgan D. R. other authors 2013; Epidemiology of Helicobacter pylori infection in six Latin American countries (SWOG Trial S0701). Cancer Causes Control 24:209–215 [View Article][PubMed]
     [Google Scholar]
  36. Rugge M., Genta R. M. OLGA Group 2005; Staging gastritis: an international proposal. Gastroenterology 129:1807–1808 [View Article][PubMed]
     [Google Scholar]
  37. Rugge M., Meggio A., Pennelli G., Piscioli F., Giacomelli L., De Pretis G., Graham D. Y. 2007; Gastritis staging in clinical practice: the OLGA staging system. Gut 56:631–636 [View Article][PubMed]
     [Google Scholar]
  38. Satoh K., Osawa H., Yoshizawa M., Nakano H., Hirasawa T., Kihira K., Sugano K. 2008; Assessment of atrophic gastritis using the OLGA system. Helicobacter 13:225–229 [View Article][PubMed]
     [Google Scholar]
  39. Shak J. R., Sodikoff J. B., Speckman R. A., Rollin F. G., Chery M. P., Cole C. R., Suchdev P. S. 2011; Anemia and Helicobacter pylori seroreactivity in a rural Haitian population. Am J Trop Med Hyg 85:913–918 [View Article][PubMed]
     [Google Scholar]
  40. Shiota S., Mahachai V., Vilaichone R. K., Ratanachu-ek T., Tshering L., Uchida T., Matsunari O., Yamaoka Y. 2013; Seroprevalence of Helicobacter pylori infection and gastric mucosal atrophy in Bhutan, a country with a high prevalence of gastric cancer. J Med Microbiol 62:1571–1578 [View Article][PubMed]
     [Google Scholar]
  41. Strebel K., Rolle-Kampczyk U., Richter M., Kindler A., Richter T., Schlink U. 2010; A rigorous small area modelling-study for the Helicobacter pylori epidemiology. Sci Total Environ 408:3931–3942 [View Article][PubMed]
     [Google Scholar]
  42. Suerbaum S., Michetti P. 2002; Helicobacter pylori infection. N Engl J Med 347:1175–1186 [View Article][PubMed]
     [Google Scholar]
  43. Sugimoto M., Yamaoka Y. 2009; The association of vacA genotype and Helicobacter pylori-related disease in Latin American and African populations. Clin Microbiol Infect 15:835–842 [View Article][PubMed]
     [Google Scholar]
  44. Sugimoto M., Zali M. R., Yamaoka Y. 2009; The association of vacA genotypes and Helicobacter pylori-related gastroduodenal diseases in the Middle East. Eur J Clin Microbiol Infect Dis 28:1227–1236 [View Article][PubMed]
     [Google Scholar]
  45. Suzuki R., Shiota S., Yamaoka Y. 2012; Molecular epidemiology, population genetics, and pathogenic role of Helicobacter pylori . Infect Genet Evol 12:203–213 [View Article][PubMed]
     [Google Scholar]
  46. Tonkic A., Tonkic M., Lehours P., Mégraud F. 2012; Epidemiology and diagnosis of Helicobacter pylori infection. Helicobacter 17:Suppl 11–8 [View Article][PubMed]
     [Google Scholar]
  47. Torres L. E., Melián K., Moreno A., Alonso J., Sabatier C. A., Hernández M., Bermúdez L., Rodríguez B. L. 2009; Prevalence of vacA, cagA and babA2 genes in Cuban Helicobacter pylori isolates. World J Gastroenterol 15:204–210 [View Article][PubMed]
     [Google Scholar]
  48. Torres L. E., González L., Melián K., Alonso J., Moreno A., Hernández M., Reyes O., Bermúdez L., Campos J. other authors 2012; EPIYA motif patterns among Cuban Helicobacter pylori CagA positive strains. Biomedica 32:23–31[PubMed]
     [Google Scholar]
  49. Tummuru M. K., Cover T. L., Blaser M. J. 1993; Cloning and expression of a high-molecular-mass major antigen of Helicobacter pylori: evidence of linkage to cytotoxin production. Infect Immun 61:1799–1809[PubMed]
     [Google Scholar]
  50. Uchida T., Kanada R., Tsukamoto Y., Hijiya N., Matsuura K., Yano S., Yokoyama S., Kishida T., Kodama M. other authors 2007; Immunohistochemical diagnosis of the cagA-gene genotype of Helicobacter pylori with anti-East Asian CagA-specific antibody. Cancer Sci 98:521–528 [View Article][PubMed]
     [Google Scholar]
  51. Uemura N., Okamoto S., Yamamoto S., Matsumura N., Yamaguchi S., Yamakido M., Taniyama K., Sasaki N., Schlemper R. J. 2001; Helicobacter pylori infection and the development of gastric cancer. N Engl J Med 345:784–789 [View Article][PubMed]
     [Google Scholar]
  52. van Doorn L. J., Figueiredo C., Sanna R., Plaisier A., Schneeberger P., de Boer W., Quint W. 1998; Clinical relevance of the cagA, vacA, and iceA status of Helicobacter pylori . Gastroenterology 115:58–66 [View Article][PubMed]
     [Google Scholar]
  53. Vilaichone R. K., Mahachai V., Tumwasorn S., Wu J. Y., Graham D. Y., Yamaoka Y. 2004; Molecular epidemiology and outcome of Helicobacter pylori infection in Thailand: a cultural cross roads. Helicobacter 9:453–459 [View Article][PubMed]
     [Google Scholar]
  54. Vilaichone R. K., Mahachai V., Shiota S., Uchida T., Ratanachu-ek T., Tshering L., Tung N. L., Fujioka T., Moriyama M., Yamaoka Y. 2013; Extremely high prevalence of Helicobacter pylori infection in Bhutan. World J Gastroenterol 19:2806–2810 [View Article][PubMed]
     [Google Scholar]
  55. Xia Y., Yamaoka Y., Zhu Q., Matha I., Gao X. 2009; A comprehensive sequence and disease correlation analyses for the C-terminal region of CagA protein of Helicobacter pylori. . PLoS ONE 4:e7736 [View Article][PubMed]
     [Google Scholar]
  56. Yamaoka Y. 2010; Mechanisms of disease: Helicobacter pylori virulence factors. Nat Rev Gastroenterol Hepatol 7:629–641[PubMed]
     [Google Scholar]
  57. Yamaoka Y., Kodama T., Kashima K., Graham D. Y., Sepulveda A. R. 1998a; Variants of the 3′ region of the cagA gene in Helicobacter pylori isolates from patients with different H. pylori-associated diseases. J Clin Microbiol 36:2258–2263[PubMed]
     [Google Scholar]
  58. Yamaoka Y., Kodama T., Kita M., Imanishi J., Kashima K., Graham D. Y. 1998b; Relationship of vacA genotypes of Helicobacter pylori to cagA status, cytotoxin production, and clinical outcome. Helicobacter 3:241–253 [View Article][PubMed]
     [Google Scholar]
  59. Yamaoka Y., El-Zimaity H. M., Gutierrez O., Figura N., Kim J. K., Kodama T., Kashima K., Graham D. Y. 1999a; Relationship between the cagA 3′ repeat region of Helicobacter pylori, gastric histology, and susceptibility to low pH. Gastroenterology 117:342–349 [View Article][PubMed]
     [Google Scholar]
  60. Yamaoka Y., Kodama T., Gutierrez O., Kim J. G., Kashima K., Graham D. Y. 1999b; Relationship between Helicobacter pylori iceA, cagA, and vacA status and clinical outcome: studies in four different countries. J Clin Microbiol 37:2274–2279[PubMed]
     [Google Scholar]
  61. Yamaoka Y., Osato M. S., Sepulveda A. R., Gutierrez O., Figura N., Kim J. G., Kodama T., Kashima K., Graham D. Y. 2000; Molecular epidemiology of Helicobacter pylori: separation of H. pylori from East Asian and non-Asian countries. Epidemiol Infect 124:91–96 [View Article][PubMed]
     [Google Scholar]
  62. Yamaoka Y., Kikuchi S., el-Zimaity H. M., Gutierrez O., Osato M. S., Graham D. Y. 2002; Importance of Helicobacter pylori oipA in clinical presentation, gastric inflammation, and mucosal interleukin 8 production. Gastroenterology 123:414–424 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.075275-0
Loading
Virulence genes of Helicobacter pylori in the Dominican Republic
J. Med. Microbiol. 63, 1189 (2014); https://doi.org/10.1099/jmm.0.075275-0
/content/journal/jmm/10.1099/jmm.0.075275-0
/content/journal/jmm/10.1099/jmm.0.075275-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error