Targeting of Genes to Specific Sites in the Mammalian Genome
This extract was created in the absence of an abstract.
Excerpt
Gene targeting — the homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences — provides the means for systematically altering the mammalian genome (Lin et al. 1985; Smithies et al. 1985; Thomas et al. 1986). A desired alteration would first be introduced into a cloned DNA sequence, and gene targeting would then transfer the alteration into the genome. Gene targeting should be equally effective for correcting or mutating the desired chromosomal locus.
We have initiated our analysis of gene targeting in cultured mammalian cells by studying recombination of a defective gene residing in the chromosome with newly introduced plasmid DNA carrying a different mutation in the same gene (Thomas et la. 1986). For these experiments, we first established cell lines containing a mutant neomycin-resistance gene (neo) integrated into the genome of mouse L cells and then sought to specifically restore the gene via homologous recombination...
