Pleiotropic control of glucose and hormone responses by PRL1, a nuclear WD protein, in Arabidopsis
- Kinga Németh1,
- Klaus Salchert1,
- Péter Putnoky1,4,
- Rishikesh Bhalerao1,
- Zsuzsanna Koncz-Kálmán1,
- Biljana Stankovic-Stangeland1,
- László Bakó1,
- Jaideep Mathur1,
- László Ökrész5,
- Sylvia Stabel2,
- Peter Geigenberger3,
- Mark Stitt3,
- George P. Rédei6,
- Jeff Schell1, and
- Csaba Koncz1,5,7
- 1Abteilung Genetische Grundlagen der Pflanzenzüchtung, and 2Max-Delbrück Laboratory, Max-Planck Institut für Züchtungsforschung, D-50829 Köln; 3Botanisches Institut, Ruprecht-Karls-Universität, D-69120 Heidelberg, Federal Republic of Germany; 4Institute of Genetics, and 5Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, H-6701 Szeged, Hungary; 6Private Investigator, Columbia, Missouri 65203-0906 USA
Abstract
The prl1 mutation localized by T-DNA tagging onArabidopsis chromosome 4-44 confers hypersensitivity to glucose and sucrose. The prl1 mutation results in transcriptional derepression of glucose responsive genes defining a novel suppressor function in glucose signaling. The prl1 mutation also augments the sensitivity of plants to growth hormones including cytokinin, ethylene, abscisic acid, and auxin; stimulates the accumulation of sugars and starch in leaves; and inhibits root elongation. PRL1encodes a regulatory WD protein that interacts with ATHKAP2, an α-importin nuclear import receptor, and is imported into the nucleus in Arabidopsis. Potential functional conservation of PRL1 homologs found in other eukaryotes is indicated by nuclear localization of PRL1 in monkey COS-1 cells and selective interaction of PRL1 with a nuclear protein kinase C–βII isoenzyme involved in human insulin signaling.
Keywords
Footnotes
-
↵7 Corresponding author.
-
E-MAIL koncz{at}mpiz-koeln.mpg.de; FAX 49-221-5062-213.
-
- Received June 23, 1998.
- Accepted August 6, 1998.
- Cold Spring Harbor Laboratory Press
