Specific functional interactions of nucleotides at key -3 and +4 positions flanking the initiation codon with components of the mammalian 48S translation initiation complex
Abstract
Eukaryotic initiation factor (eIF) 1 maintains the fidelity of initiation codon selection and enables mammalian 43S preinitiation complexes to discriminate against AUG codons with a context that deviates from the optimum sequence GCC(A/G)CCAUGG, in which the purines at -3 and +4 positions are most important. We hypothesize that eIF1 acts by antagonizing conformational changes that occur in ribosomal complexes upon codon-anticodon base-pairing during 48S initiation complex formation, and that the role of -3 and +4 context nucleotides is to stabilize these changes by interacting with components of this complex. Here we report that U and G at +4 both UV-cross-linked to ribosomal protein (rp) S15 in 48S complexes. However, whereas U cross-linked strongly to C1696 and less well to AA1818-1819 in helix 44 of 18S rRNA, G cross-linked exclusively to AA1818-1819. U at -3 cross-linked to rpS5 and eIF2α, whereas G cross-linked only to eIF2α. Results of UV cross-linking experiments and of assays of 48S complex formation done using α-subunit-deficient eIF2 indicate that eIF2α's interaction with the -3 purine is responsible for recognition of the -3 context position by 43S complexes and suggest that the +4 purine/AA1818-1819 interaction might be responsible for recognizing the +4 position.
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Footnotes
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Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.1397906.
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↵3 Corresponding author. E-MAIL tatyana.pestova{at}downstate.edu; FAX (718) 270-2656.
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- Accepted January 13, 2006.
- Received December 2, 2005.
- Cold Spring Harbor Laboratory Press