Optical Mapping of BAC Clones from the Human Y Chromosome DAZ Locus
- Joseph Giacalone1,
- Stephanie Delobette1,
- Veronica Gibaja1,
- Lei Ni1,4,
- Yiannis Skiadas1,5,
- Rong Qi1,6,
- Joanne Edington1,7,
- Zhongwu Lai1,8,
- Damara Gebauer1,
- Hongjuan Zhao1,
- Thomas Anantharaman2,
- Bhubaneswar Mishra2,
- Laura G. Brown3,
- Richa Saxena3,
- David C. Page3, and
- David C. Schwartz4,9
- 1W.M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, New York, New York 10003, USA; 2Courant Institute of Mathematical Sciences, New York University, Department of Computer Science, New York, New York 10012, USA; 3Howard Hughes Medical Institute, Whitehead Institute, and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA; 4Departments of Genetics and Chemistry, UW–Biotechnology Center, University of Wisconsin–Madison, Madison, Wisconsin 53706, USA
Abstract
The accurate mapping of clones derived from genomic regions containing complex arrangements of repeated elements presents special problems for DNA sequencers. Recent advances in the automation of optical mapping have enabled us to map a set of 16 BAC clones derived from the DAZ locus of the human Y chromosome long arm, a locus in which the entire DAZ gene as well as subsections within the gene copies have been duplicated. High-resolution optical mapping employing seven enzymes places these clones into two contigs representing four distinct copies of the DAZ gene and highlights a number of differences between individual copies of DAZ.
Footnotes
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↵5 Present address: University of California–Davis, Davis, CA 95616, USA.
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↵6 Present address: The Institute for Genomic Research, Rockville, MD 20850, USA.
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↵7 Present address: The Rockefeller University, New York, NY 10021, USA.
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↵8 Present address: Celera Genomics, Rockville, MD 20850, USA.
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↵9 Corresponding author.
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E-MAIL dcschwartz{at}facstaff.wisc.edu; FAX (608) 265-6743.
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Article and publication are at www.genome.org/cgi/doi/10.1101/gr.112100.
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- Received June 25, 1999.
- Accepted July 12, 2000.
- Cold Spring Harbor Laboratory Press
