A BAC-Based Physical Map of the Chicken Genome
Abstract
A genome-wide physical map constructed with bacterial artificial chromosomes (BACs) is an essential component in linking phenotypic traits to the responsible genetic variation in the genomes of plants and animals. We have constructed a physical map of the chicken genome from 57,091 BACs (7.9-fold haploid genome coverage) by restriction fingerprint analysis using high-resolution polyacrylamide gel electrophoresis. The physical map consists of 2331 overlapping BAC contigs and is estimated to span 1510 Mb in physical length. BAC contigs were verified manually and by screening the BACs with 367 DNA markers. A total of 361 of the contigs have been anchored to the existing chicken genetic map. This map represents the first genome-wide, BAC-based physical map of the chicken genome. It provides a powerful platform for many areas of chicken genomics, including targeted marker development, fine mapping of genes and QTL alleles, positional cloning, analysis of avian genome organization and evolution, chicken-mammalian comparative genomics, and large-scale genome sequencing.
Footnotes
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[Supplemental materials, including the three source BAC libraries, clone fingerprints, and contig map, are available online at http://hbz.tamu.edu. The database of BAC clones associated with DNA markers is available online at http://poultry.mph.msu.edu/resources/Resources.htm#bacdata.]
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Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.1499303.
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↵3 These authors contributed equally to this work.
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↵4 Corresponding author. E-MAIL hbz7049{at}tamu.edu; FAX (979) 862-4790.
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- Accepted September 3, 2003.
- Received May 5, 2003.
- Cold Spring Harbor Laboratory Press
