A Versatile Assay for High-Throughput Gene Expression Profiling on Universal Array Matrices
Abstract
We report a flexible, sensitive, and quantitative gene-expression profiling system for assaying more than 400 genes, with three probes per gene, for 96 samples in parallel. The cDNA-mediated annealing, selection, extension and ligation (DASL) assay targets specific transcripts, using oligonucleotides containing unique address sequences that can hybridize to universal arrays. Cell-specific gene expression profiles were obtained using this assay for hormone-treated cell lines and laser-capture microdissected cancer tissues. Gene expression profiles derived from this assay were consistent with those determined by qRT-PCR. The DASL assay has been automated for use with a bead-based 96-array matrix system. The combined high-throughput assay and readout system is accurate and efficient, and can cost-effectively profile the expression of hundreds of genes in thousands of samples.
Footnotes
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Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.2167504.
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↵3 Corresponding author. E-MAIL jfan{at}illumina.com; FAX (858) 202-4680.
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- Accepted February 11, 2004.
- Received November 13, 2003.
- Cold Spring Harbor Laboratory Press