1274 Full-Open Reading Frames of Transcripts Expressed in the Developing Mouse Nervous System
- Maria F. Bonaldo1,
- Thomas B. Bair2,
- Todd E. Scheetz2,3,
- Einat Snir1,
- Ike Akabogu1,
- Jennifer L. Bair1,
- Brian Berger1,
- Keith Crouch1,
- Aja Davis1,
- Mari E. Eyestone1,
- Catherine Keppel1,
- Tamara A. Kucaba1,
- Mark Lebeck1,
- Jenny L. Lin4,
- Anna I.R. de Melo1,
- Joshua Rehmann1,
- Rebecca S. Reiter4,
- Kelly Schaefer1,
- Christina Smith1,
- Dylan Tack5,
- Kurtis Trout1,
- Val C. Sheffield1,6,
- Jim J-C. Lin4,
- Thomas L. Casavant2,3,5,7, and
- Marcelo B. Soares1,8,9,10,11
- 1 Department of Pediatrics, The University of Iowa, Iowa City, Iowa 52242, USA
- 2 Department of Center for Bioinformatics and Computational Biology, The University of Iowa, Iowa City, Iowa 52242, USA
- 3 Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, Iowa 52242, USA
- 4 Department of Biological Sciences, The University of Iowa, Iowa City, Iowa 52242, USA
- 5 Department of Electrical and Computer Engineering, The University of Iowa, Iowa City, Iowa 52242, USA
- 6 Department of Howard Hughes Medical Institute, The University of Iowa, Iowa City, Iowa 52242, USA
- 7 Department of Biomedical Engineering, The University of Iowa, Iowa City, Iowa 52242, USA
- 8 Department of Biochemistry, The University of Iowa, Iowa City, Iowa 52242, USA
- 9 Department of Physiology and Biophysics, The University of Iowa, Iowa City, Iowa 52242, USA
- 10 Department of Orthopaedics, The University of Iowa, Iowa City, Iowa 52242, USA
Abstract
As part of the trans-National Institutes of Health (NIH) Mouse Brain Molecular Anatomy Project (BMAP), and in close coordination with the NIH Mammalian Gene Collection Program (MGC), we initiated a large-scale project to clone, identify, and sequence the complete open reading frame (ORF) of transcripts expressed in the developing mouse nervous system. Here we report the analysis of the ORF sequence of 1274 cDNAs, obtained from 47 full-length-enriched cDNA libraries, constructed by using a novel approach, herein described. cDNA libraries were derived from size-fractionated cytoplasmic mRNA isolated from brain and eye tissues obtained at several embryonic stages and postnatal days. Altogether, including the full-ORF MGC sequences derived from these libraries by the MGC sequencing team, NIH_BMAP full-ORF sequences correspond to ∼20% of all transcripts currently represented in mouse MGC. We show that NIH_BMAP clones comprise 68% of mouse MGC cDNAs ≥5 kb, and 54% of those ≥4 kb, as of March 15, 2004. Importantly, we identified transcripts, among the 1274 full-ORF sequences, that are exclusively or predominantly expressed in brain and eye tissues, many of which encode yet uncharacterized proteins.
Footnotes
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[Supplemental material is available online at www.genome.org.]
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Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.2601304.
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↵11 Corresponding author. E-MAIL bento-soares{at}uiowa.edu; FAX (319) 335-9565.
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- Accepted April 27, 2004.
- Received March 29, 2004.
- Cold Spring Harbor Laboratory Press