ORFeome Cloning and Systems Biology: Standardized Mass Production of the Parts From the Parts-List

  1. Michael A. Brasch1,
  2. James L. Hartley2, and
  3. Marc Vidal3,4
  1. 1 Atto Bioscience, Rockville, Maryland 20850, USA
  2. 2 SAIC/National Cancer Institute, Frederick, Maryland 21702, USA
  3. 3 Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA

Abstract

Together with metabolites, proteins and RNAs form complex biological systems through highly intricate networks of physical and functional interactions. Large-scale studies aimed at a molecular understanding of the structure, function, and dynamics of proteins and RNAs in the context of cellular networks require novel approaches and technologies. This Special Issue of Genome Research features strategies for the high-throughput construction and manipulation of complete sets of protein-encoding open reading frames (ORFeome), gene promoters (promoterome), and noncoding RNAs, as predicted from genome and transcriptome sequences. Here we discuss the use of a recombinational cloning system that allows efficiency, adaptability, and compatibility in the generation of ORFeome, promoterome, and other resources.

Footnotes

  • Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.2769804.

  • ↵4 Corresponding author. E-MAIL marc_vidal{at}dfci.harvard.edu; FAX (617) 632-5739.

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  1. doi: 10.1101/gr.2769804 Genome Res. 14: 2001-2009 Cold Spring Harbor Laboratory Press

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