Novel noncoding RNA from human Y distal heterochromatic block (Yq12) generates testis-specific chimeric CDC2L2
- Zeenath Jehan1,3,
- Sambandam Vallinayagam1,3,
- Shrish Tiwari1,
- Suman Pradhan1,
- Lalji Singh1,
- Amritha Suresh1,
- Hemakumar M. Reddy1,
- Y.R. Ahuja2, and
- Rachel A. Jesudasan1,4
- 1 Centre for Cellular and Molecular Biology, Uppal Road Hyderabad–500 007, AP, India;
- 2 Genetics Unit, Vasavi Medical and Research Centre, Hyderabad, India, AP, India
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↵3 These two authors contributed equally to this work.
Abstract
The human Y chromosome, because it is enriched in repetitive DNA, has been very intractable to genetic and molecular analyses. There is no previous evidence for developmental stage- and testis-specific transcription from the male-specific region of the Y (MSY). Here, we present evidence for the first time for a developmental stage- and testis-specific transcription from MSY distal heterochromatic block. We isolated two novel RNAs, which localize to Yq12 in multiple copies, show testis-specific expression, and lack active X-homologs. Experimental evidence shows that one of the above Yq12 noncoding RNAs (ncRNAs) trans-splices with CDC2L2 mRNA from chromosome 1p36.3 locus to generate a testis-specific chimeric β sv13 isoform. This 67-nt 5′UTR provided by the Yq12 transcript contains within it a Y box protein-binding CCAAT motif, indicating translational regulation of the β sv13 isoform in testis. This is also the first report of trans-splicing between a Y chromosomal and an autosomal transcript.
Footnotes
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↵4 Corresponding author.
↵4 E-mail rachel{at}ccmb.res.in; fax 91-40-27160311.
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[The sequence data from this study have been submitted to GenBank under accession nos. AY598345, AY598346, and AY598347.]
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Article published online before print. Article and publication date are at http://www.genome.org/cgi/doi/10.1101/gr.5155706
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- Received January 19, 2006.
- Accepted September 6, 2006.
- Copyright © 2007, Cold Spring Harbor Laboratory Press
