Immunohistochemistry Staining of Embryos from the African Butterfly Bicyclus anynana
- ↵1Corresponding author (p.m.brakefield{at}biology.leidenuniv.nl)
INTRODUCTION
This protocol describes an immunohistochemical method for the detection of proteins in developing embryos of the African butterfly Bicyclus anynana. The embryos are stained with a primary antibody against a protein of interest, followed by a fluorescently labeled secondary antibody.