Recipe

LB (Luria-Bertani) liquid medium

Reagent Amount to add
H2O 950 mL
Tryptone 10 g
NaCl 10 g
Yeast extract 5 g
Combine the reagents and shake until the solutes have dissolved. Adjust the pH to 7.0 with 5 N NaOH (~0.2 mL). Adjust the final volume of the solution to 1 L with H2O. Sterilize by autoclaving for 20 min at 15 psi (1.05 kg/cm2) on liquid cycle.
For solid medium, see the recipe entitled “Media containing agar or agarose.”

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  1. doi:10.1101/pdb.rec8141 Cold Spring Harb Protoc 2006: pdb.rec8141-
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