A physiological role for gene loops in yeast
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↵1 These authors contributed equally to this work.
Abstract
DNA loops that juxtapose the promoter and terminator regions of RNA polymerase II-transcribed genes have been identified in yeast and mammalian cells. Loop formation is transcription-dependent and requires components of the pre-mRNA 3′-end processing machinery. Here we report that looping at the yeast GAL10 gene persists following a cycle of transcriptional activation and repression. Moreover, GAL10 and a GAL1p-SEN1 reporter undergo rapid reactivation kinetics following a cycle of activation and repression—a phenomenon defined as “transcriptional memory”—and this effect correlates with the persistence of looping. We propose that gene loops facilitate transcriptional memory in yeast.
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Footnotes
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↵2 Corresponding author.
E-MAIL michael.hampsey{at}umdnj.edu; FAX (732) 235-5889.
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Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.1823609.
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Supplemental material is available at http://www.genesdev.org.
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- Received May 21, 2009.
- Accepted September 22, 2009.
- Copyright © 2009 by Cold Spring Harbor Laboratory Press
