Varying Intertrial Interval Reveals Temporally Defined Memory Deficits and Enhancements in NTAN1-Deficient Mice
Abstract
The N-end rule is one ubiquitin-proteolytic pathway that relates the in vivo half-life of a protein to the identity of its N-terminal residue. NTAN1 deamidates N-terminal asparagine to aspartate, which is conjugated to arginine by ATE1. An N-terminal arginine-bearing substrate protein is recognized, ubiquitylated by UBR1/E3α, and subsequently degraded by 26S proteasomes. Previous research showed that NTAN1-deficient mice exhibited impaired long-term memory in the Lashley III maze. Therefore, a series of studies, designed to assess the role of NTAN1 in short- and intermediate-term memory processes, was undertaken. Two hundred sixty mice (126 −/−; 134 +/ +) received Lashley III maze training with intertrial intervals ranging from 2–180 min. Results indicated that inactivation of NTAN1 amidase differentially affects short-, intermediate-, and long-term memory.
Footnotes
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↵4 Present address: University of Connecticut, Biobehavioral Sciences, Graduate Degree Program, 3107 Horsebarn Hill Road, U-154, Storrs, CT 06269-4154, USA.
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↵5 Corresponding author.
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E-MAIL dberg{at}uconnvm.uconn.edu; FAX (860) 486-3827.
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Article and publication are atwww.learnmem.org/cgi/doi/10.1101/lm.33500.
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- Received May 16, 2000.
- Accepted August 2, 2000.
- Cold Spring Harbor Laboratory Press
