Biofunctionalization of Si nanowires using a solution based technique

Elissa H. Williams; Albert V. Davydov; Vladimir P. Oleshko; Nancy J. Lin; Kristen L. Steffens; Amy K. Manocchi; Sergiy Krylyuk; Mulpuri V. Rao; John A. Schreifels

doi:10.1117/12.970460

19 October 2012 Biofunctionalization of Si nanowires using a solution based technique

Elissa H. Williams, Albert V. Davydov, Vladimir P. Oleshko, Nancy J. Lin, Kristen L. Steffens, Amy K. Manocchi, Sergiy Krylyuk, Mulpuri V. Rao, John A. Schreifels

Author Affiliations +

Proceedings Volume 8467, Nanoepitaxy: Materials and Devices IV; 846702 (2012) https://doi.org/10.1117/12.970460
Event: SPIE NanoScience + Engineering, 2012, San Diego, California, United States

Abstract

Here we present a solution based functionalization technique for streptavidin (SA) protein conjugation to silicon nanowires (Si NWs). Si NWs, with a diameter of 110 nm to 130 nm and a length of 5 μm to 10 μm, were functionalized with 3-aminopropyltriethoxysilane (APTES) followed by biotin for the selective attachment of SA. High-resolution transmission electron microscopy (HRTEM) and atomic force microscopy (AFM) showed that the Si NWs were conformally coated with 20 nm to 30 nm thick APTES, biotin, and SA layers upon functionalization. Successful attachment of each bio/organic layer was confirmed by X-ray photoelectron spectroscopy (XPS) and fluorescence microscopy. Fluorescence microscopy also demonstrated that there was an undesirable non-specific binding of the SA protein as well as a control protein, bovine serum albumin (BSA), to the APTES-coated Si NWs. However, inhibition of BSA binding and enhancement of SA binding were achieved following the biotinylation step. The biofunctionalized Si NWs show potential as label-free biosensing platforms for the specific and selective detection of biomolecules.

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Elissa H. Williams, Albert V. Davydov, Vladimir P. Oleshko, Nancy J. Lin, Kristen L. Steffens, Amy K. Manocchi, Sergiy Krylyuk, Mulpuri V. Rao, and John A. Schreifels "Biofunctionalization of Si nanowires using a solution based technique", Proc. SPIE 8467, Nanoepitaxy: Materials and Devices IV, 846702 (19 October 2012); https://doi.org/10.1117/12.970460

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