Molecular Evolution of Protein Atomic Composition
Abstract
Living organisms encounter various growth conditions in their habitats, raising the question of whether ecological fluctuations could alter biological macromolecules. The advent of complete genome sequences and the characterization of whole metabolic pathways allowed us to search for such ecological imprints. Significant correlations between atomic composition and metabolic function were found in sulfur- and carbon-assimilatory enzymes, which appear depleted in sulfur and carbon, respectively, in both the bacterium Escherichia coliand the eukaryote Saccharomyces cerevisiae. In addition to genetic instructions, genomic data thus also provide paleontological records of environmental nutrient availability and of metabolic costs.
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Supplementary Material
REFERENCES AND NOTES
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For S. cerevisiae, the sulfur-assimilatory enzymes used for the statistical analyses were the MET1, 2, 3, 4, 5, 6, 7, 8, 10, 13, 14, 16, 25, 28, 31, 32, STR1, STR4, SUL1, and SUL2 gene products. For E. coli, the enzymes used were the CysA, B, C, D, E, G, H, I, J, K, M, N, P, U, W, Z, MetA, B, C, E, H, R, and Sbp gene products.
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For S. cerevisiae, the carbon-assimilatory enzymes used for the statistical analyses were the ENO1, ENO2, FBA1, FBP1, GLK1, GMP1, HXK1, HXK2, PDC1, PDC5, PDC6, PFK1, PFK2, PGI1, PGK1, PYC1, PYK1, TDH1, TDH2, TDH3, TPI1, and ZWF1 gene products. For E. coli, the enzymes used were the Eno, FbaA, GapA, Glk, Gnd, GpmA, PfkA, PfkB, Pgi, Pgk, Pgm, PykA, RpE, RpiA, RpiB, TalB, TktA, TktB, TpiA, and Zwf gene products.
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The S. cerevisiae nitrogen-assimilatory enzymes used for the statistical analyses were the DAL1, 2, 3, 4, 5, 7, 81, DUR3, DUR12, GDH1, GDH2, PUT1, 2, 3, 4, UGA1, 3, 4, and 5 gene products.
27
Supported by a thesis fellowship from the Ministère de la Défense (P.B.-C.).
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Published In
Science
Volume 293 | Issue 5528
13 July 2001
13 July 2001
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Received: 26 March 2001
Accepted: 1 June 2001
Published in print: 13 July 2001
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- Peggy Baudouin-Cornu et al.
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