Original paper
Identification of carboxylesterase genes contributing to multi-insecticide resistance in Plutella xylostella (L.)
Li, Ran; Zhu, Bin; Liang, Pei; Gao, Xiwu
Entomologia Generalis Volume 42 Number 6 (2022), p. 967 - 976
published: Dec 8, 2022
published online: Oct 24, 2022
manuscript accepted: Aug 23, 2022
manuscript revision received: Feb 12, 2022
manuscript revision requested: Feb 1, 2022
manuscript received: Jan 29, 2022
DOI: 10.1127/entomologia/2022/1572
ArtNo. ESP146004206014, Price: 29.00 €
Abstract
Carboxylesterases (CarEs) are one of the major enzyme families associated with the detoxification of ester-containing xenobiotics including insecticides. The contribution of CarEs to single insecticide resistance have been reported widely. However, little is known about the roles of CarEs in multi-insecticide resistance. In this study, a total of 46 genes were identified in CarE family of Plutella xylostella (L.), a notorious pest of cruciferous crops, of which 21 genes were found overexpressed in field collected Hainan (HN) and Guangdong (GD) populations which showed moderate to extremely high resistance to beta-cypermethrin, chlorantraniliprole, metaflumizone, phoxim and tebufenozide. Among the 21 overexpressed CarE genes, PxαE3, PxαE6, PxαE9, PxαE13 and PxαE27 can be upregulated significantly by LC25 of the five insecticides. Knockdown of the five genes by RNA interference and the followed bioassays revealed that PxαE3 was involved in chlorantraniliprole resistance, PxαE6, PxαE9 and PxαE13 to phoxim resistance, PxαE9 and PxαE27 to beta-cypermethrin resistance, respectively. The bioassay results with transgenic fruit flies overexpressing each of the five genes confirmed their roles in resistance to the three insecticides. Our results demonstrated that at least five carboxylesterase genes were involved in multi-insecticide resistance in P. xylostella through constitutive and inductive overexpression, and provided foundations for further understanding the molecular mechanisms of multi-insecticide resistance in pest insects.
Keywords
diamondback moth • carboxylesterase overexpression • insecticide resistance • detoxification • chlorantraniliprole