EFFECT OF INCUBATION TEMPERATURE ON DENGUE-2 VIRAL ANTIGEN PRODUCTION AND VIRAL RNA SYNTHESIS IN INFECTED Aedes albopictus CLONE C6/36 CELL LINE

Marlou Noel M. MANGADA; Halimah MOHAMED; Leticia del Carmen CASTILLO; Futoshi HASEBE; Akira IGARASHI

doi:10.11418/jtca1981.14.4_213

EFFECT OF INCUBATION TEMPERATURE ON DENGUE-2 VIRAL ANTIGEN PRODUCTION AND VIRAL RNA SYNTHESIS IN INFECTED Aedes albopictus CLONE C6/36 CELL LINE

Marlou Noel M. MANGADA, Halimah MOHAMED, Leticia del Carmen CASTILLO, Futoshi HASEBE, Akira IGARASHI

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Keywords: Dengue virus antigens, Aedes albopictus clone C6/36 cell line, incubation temperature, quantitative reverse transcriptase-polymerase chain reaction, internal control

JOURNAL FREE ACCESS

1995 Volume 14 Issue 4 Pages 213-220

DOI https://doi.org/10.11418/jtca1981.14.4_213

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Abstract

Increase in the incubation temperature of infected Aedes albopictus clone C6/36cells from its optimal growth temperature of 28°C to 32 and 37°C showed a concomitant rise in the production of dengue virus type 2 antigen and genomic RNA. The quantitative reverse transcription-polymerase chain reaction using an internal standard as a measuring tool for the viral genomic RNA showed that RNA production was higher in the cells and in the extracellular fluid from the cultures incubated at 32 and 37°C than at 28°C. The presence of viral antigen and viral genomic RNA was also detected 1-2days earlier in the cultures incubated at elevated temperatures.

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