不同密度甘肃马先蒿寄生和内生真菌互作对紫花针茅内源激素及生物碱含量的影响

doi:10.11686/cyxb2019313

草业学报 ›› 2020, Vol. 29 ›› Issue (4): 147-156.DOI: 10.11686/cyxb2019313

不同密度甘肃马先蒿寄生和内生真菌互作对紫花针茅内源激素及生物碱含量的影响

鲍根生^1,2,*, 宋梅玲^1,2, 王玉琴^1,2, 刘静³, 王宏生^1,2

1.青海大学畜牧兽医科学院,青海西宁 810016;
2.青海大学省部共建三江源生态与高原农牧业国家重点实验室,青海西宁 810003;
3.兰州大学草地农业生态系统国家重点实验室,甘肃兰州 730020

收稿日期:2019-07-08 修回日期:2019-10-10 出版日期:2020-04-20 发布日期:2020-04-20
通讯作者: E-mail: baogensheng2008@hotmail.com
作者简介:鲍根生(1980-),男,青海乐都人,博士。E-mail: baogensheng2008@hotmail.com
基金资助:
国家自然科学基金项目(31660690, 31700098)和青海省“高端创新人才千人计划”拔尖人才培养计划项目资助

Interactive effects of different densities of Pedicularis kansuensis parasitism and Epichloё endophyte infection on the endogenous hormone levels and alkaloid contents of Stipa purpurea

BAO Gen-sheng^1,2,*, SONG Mei-ling^1,2, WANG Yu-qin^1,2, LIU Jing³, WANG Hong-sheng^1,2

1.Academy of Animal and Veterinary Medicine, Qinghai University, Xining 810016, China;
2.State Key Laboratory Plateau Ecology and Agriculture, Qinghai University, Xining 810003, China;
3.Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China

Received:2019-07-08 Revised:2019-10-10 Online:2020-04-20 Published:2020-04-20
Contact: E-mail: baogensheng2008@hotmail.com

摘要/Abstract

摘要： 根寄生植物是禾草生长过程中的一种生物逆境,禾草内生真菌能增强其对生物或非生物逆境的耐受能力;然而,有关禾草内生真菌对不同密度根寄生逆境下禾草生长、内源激素水平和生物碱含量变化的研究鲜有报道。基于此,通过开展温室盆栽试验,以建立根寄生关系的带菌(E+)和未带菌(E-)紫花针茅及根寄生植物-甘肃马先蒿为研究对象,比较E+和E-紫花针茅在不同甘肃马先蒿寄生强度下生长、内源激素和生物碱含量变化。结果表明,与未寄生紫花针茅相比,随着甘肃马先蒿密度增加,紫花针茅生物量降低80%,株高、分蘖数和根长均降低25%,吲哚乙酸、脱落酸含量分别增加43%和51%,细胞分裂素含量降低50%。内生真菌侵染显著提高紫花针茅生长特性,增加内源激素和生物碱含量;带菌紫花针茅在根寄生逆境下生物碱含量持续增加,同时甘肃马先蒿利用根部木质部通道可获取寄主内生真菌合成的生物碱。由此可见,内生真菌通过调控紫花针茅体内有关生长和逆境预警相关内源激素水平及改变生物碱含量的方式增强紫花针茅对甘肃马先蒿根寄生逆境的耐受能力,为利用禾草内生真菌共生体修复甘肃马先蒿型退化草地提供新视角。

关键词: 根寄生植物, 内源激素, 生物碱, 内生真菌, 紫花针茅, 密度

Abstract: Root hemiparasites of grasses in the natural environment impose a biotic stress on their hosts. Conversely, Epichloё endophytes enhance their hosts’ tolerance or resistance to biotic and abiotic stresses. However, only a few studies have explored the interactive effects of different densities of hemiparasite infection and the presence or absence of Epichloё endophyte on the growth, endogenous hormone levels and alkaloid contents of the host grasses. Therefore, we set up a two factor pot experiment with endophyte-infected (E+) and endophyte-free (E-) plants of Stipa purpurea as the host grass, grown without (control) or with one or three plants of the hemiparasite Pedicularis kansuensis. Various plant growth parameters and endogenous hormone levels were determined for E+ and E- S. purpurea, while alkaloid concentrations of endophyte-infected S. pupurea plants and their hemiparasites (where present) were measured. Comparing parasitized and unparasitized S. purpurea, biomass of parasitized plants was decreased 80%, plant height, tiller number and root length were reduced 25%, and indole-3-acetic acid and abscisic acid levels of S. purpurea were increased 43% and 51%, respectively. However, zeatin-riboside levels were decreased by 50%, while the growth parameters, and endogenous hormone levels of E+ host grasses were significantly higher than those of their E- counterparts. Furthermore, under parasitic stress, alkaloid production of Epichloё-endophyte-infected plants was enhanced, and alkaloids were transferred from E+ host grass plants to their root hemiparasites through the xylem bridge formed by haustoria. In conclusion, our results show that Epichloё endophyte has a potential role in improving its host grass resistance or tolerance to parasitic stress by up-regulating host grass alkaloid contents and endogenous hormone levels, especially those hormones typically sensitive to biotic stress. Our results also suggest that use of the Epichloё endophyte symbiont has potential as a tool to assist recovery of degraded grassland in the Qinghai Tibet Plateau where root hemiparasites are widely distributed.

Key words: root hemiparasite, endogenous hormone, alkaloid, Epichloë endophyte, Stipa purpurea, density

鲍根生, 宋梅玲, 王玉琴, 刘静, 王宏生. 不同密度甘肃马先蒿寄生和内生真菌互作对紫花针茅内源激素及生物碱含量的影响[J]. 草业学报, 2020, 29(4): 147-156.

BAO Gen-sheng, SONG Mei-ling, WANG Yu-qin, LIU Jing, WANG Hong-sheng. Interactive effects of different densities of Pedicularis kansuensis parasitism and Epichloё endophyte infection on the endogenous hormone levels and alkaloid contents of Stipa purpurea[J]. Acta Prataculturae Sinica, 2020, 29(4): 147-156.

参考文献

[1] Bardgett R D, Smith R S, Shiel R S, et al. Parasitic plants indirectly regulate below-ground properties in grassland ecosystems. Nature, 2006, 439(7079): 969-972.
[2] Thorogood C J, Rumsey F J, Hiscock S J. Host-specific races in the holoparasitic angiosperm Orobanche minor: Implications for speciation in parasitic plants. Annals of Botany, 2009, 103(7): 1005-1014.
[3] Joel D M, Gressel J, Musselman L J. Parasitic orobanchaceae: Parasitic mechanisms and control strategies. Berlin Heidelberg: Springer-Verlag, 2015: 75-84.
[4] Phoenix G K, Press M C. Linking physiological traits to impacts on community structure and function: The role of root hemiparasitic Orobanchaceae (ex-Scrophulariaceae). Journal of Ecology, 2005, 93(1): 67-78.
[5] Cameron D D, Coats A M, Seel W E. Differential resistance among host and non-host species underlies the variable success of the hemi-parasitic plant Rhinanthus minor. Annals of Botany, 2006, 98(6): 1289-1299.
[6] Bao G S, Suetsugu K, Wang H S, et al. Effects of the hemiparasitic plant Pedicularis kansuensis on plant community structure in a degraded grassland. Ecological Research, 2015, 30(3): 507-515.
[7] Heer N, Klimmek F, Zwahlen C, et al. Hemiparasite-density effects on grassland plant diversity, composition and biomass. Perspect Plant Ecology, 2018, 32: 22-29.
[8] Jiang F, Veselova S, Veselov D, et al. Cytokinin flows from Hordeum vulgare to the hemiparasite Rhinanthus minor and the influence of infection on host and parasite cytokinins relations. Functional Plant Biology, 2005, 32(7): 619-629.
[9] Jiang F, Jeschke W D, Hartung W, et al. Interactions between Rhinanthus minor and its hosts: A review of water, mineral nutrient and hormone flows and exchanges in the hemiparasitic association. Folia Geobotanica, 2010, 45(4): 369-385.
[10] Jiang F, Jeschke W D, Hartung W. Abscisic acid (ABA) flows from Hordeum vulgare to the hemiparasite Rhinanthus minor and the influence of infection on host and parasite abscisic acid relations. Journal of Experimental Botany, 2004, 55(406): 2323-2329.
[11] Hegenauer V, Körner M, Albert M. Plants under stress by parasitic plants. Current Opinion in Plant Biology, 2017, 38: 34-41.
[12] Santino A, Taurino M, De Domenico S, et al. Jasmonate signaling in plant development and defense response to multiple (a)biotic stresses. Plant Cell Reports, 2013, 32(7): 1085-1098.
[13] Lehtonen P, Helander M, Wink M, et al. Transfer of endophyte-origin defensive alkaloids from a grass to a hemiparasitic plant. Ecology Letters, 2005, 8(12): 1256-1263.
[14] Laitinen R K, Hellström K O, Wäli P R. Context-dependent outcomes of subarctic grass-endophyte symbiosis. Fungal Ecology, 2016, 23: 66-74.
[15] Sui X L, Zhang T, Tian Y Q, et al. A neglected alliance in battles against parasitic plants: Arbuscular mycorrhizal and rhizobial symbioses alleviate damage to a legume host by root hemiparasitic Pedicularis species. New Phytologist, 2019, 221(1): 470-481.
[16] Schardl C L, Leuchtmann A, Spiering M J. Symbioses of grasses with seedborne fungal endophytes. Annual Review of Plant Biology, 2004, 55: 315-340.
[17] Saikkonen K, Wäli P, Helander M, et al. Evolution of endophyte-plant symbioses. Trends in Plant Science, 2004, 9(6): 275-280.
[18] Fuchs B, Krauss J. Can Epichloё endophytes enhance direct and indirect plant defence? Fungal Ecology, 2019, 38: 98-103.
[19] Clay K. Fungal endophytes of grasses: A defensive mutualism between plants and fungi. Ecology, 1988, 69(1): 10-16.
[20] Saikkonen K, Gundel P E, Helander M. Chemical ecology mediated by fungal endophytes in grasses. Journal of Chemical Ecology, 2013, 39(7): 962-968.
[21] Li C J, Nan Z B, Paul V H, et al. A new Neotyphodium species symbiotic with drunken horse grass (Achnatherum inebrians) in China. Mycotaxon, 2004, 90(1): 141-147.
[22] Bao G S, Li C J. Isolation and identification eddophytes infecting Stipa purpurea, a dominant grass in meadows of the Qinghai-Tibet plateau. Acta Prataculturae Sinica, 2016, 25(3): 32-42.
鲍根生, 李春杰. 青藏高原高寒草地优势禾草-紫花针茅内生真菌分离和鉴定. 草业学报, 2016, 25(3): 32-42.
[23] Bacon C W. Abiotic stress tolerances (moisture, nutrients) and photosynthesis in endophyte-infected tall fescue. Agriculture Ecosystems & Environment, 1993, 44(1/2/3/4): 123-141.
[24] Chen Y, Guan K Y, Li A R, et al. Effects of nitrogen and phosphorus supply on root morphology of two Pedicularis species. Plant Diversity and Resources, 2014, 36(1): 56-64.
陈燕, 管开云, 李爱荣, 等. 氮磷供给对两种马先蒿根系形态建成的影响. 植物分类与资源学报, 2014, 36(1): 56-64.
[25] Sui X L, Li A R, Chen Y, et al. Arbuscular mycorrhizal fungi: Potential biocontrol agents against the damaging root hemiparasite Pedicularis kansuensis? Mycorrhiza, 2014, 24(3): 187-195.
[26] You C C, Zhu H L, Xu B B, et al. Effect of removing superior spikelets on grain filling of inferior spikelets in rice. Frontiers in Plant Science, 2016, 7: 1161.
[27] Zhou L Y, Li C J, Zhang X X, et al. Effects of cold shocked Epichloё infected Festuca sinensis on ergot alkaloid accumulation. Fungal Ecology, 2015, 14: 99-104.
[28] Siegrist J A, McCulley R L, Bush L P, et al. Alkaloids may not be responsible for endophyte-associated reductions in tall fescue decomposition rates. Functional Ecology, 2010, 24(2): 460-468.
[29] Zhang Y P, Nan Z B. Distribution of Epichloë endophytes in Chinese populations of Elymus dahuricus and variation in peramine levels. Symbiosis (Rehovot), 2007, 43(1): 13-19.
[30] Westerman P R, Hemerik L, van der Werf W, et al. Density-independent reproductive success of the hemiparasitic plant Striga hermonthica, despite positive and negative density-dependent phases. Annals of Applied Biology, 2018, 172(1): 74-87.
[31] Matthies D. Host-parasite relations in the root hemiparasite Melampyrum arvense. Flora, 1995, 190(4): 383-394.
[32] Rodenburg J, Bastiaans L, Kropff M J. Characterization of host tolerance to Striga hermonthica. Euphytica, 2006, 147(3): 353-365.
[33] Bernhard R H, Jensen J E, Andreasen C. Prediction of yield loss caused by Orobanche spp. in carrot and pea crops based on the soil seedbank. Weed Research, 1998, 38(3): 191-197.
[34] Cameron D D, Geniez J M, Seel W E, et al. Suppression of host photosynthesis by the parasitic plant Rhinanthus minor. Annals of Botany, 2008, 101(4): 573-578.
[35] Sui X L, Huang W, Li Y J, et al. Host shoot clipping depresses the growth of weedy hemiparasitic Pedicularis kansuensis. Journal of Plant Research, 2015, 128(4): 563-572.
[36] TěŠitel J. Functional biology of parasitic plants: A review. Plant Ecology and Evolution, 2016, 149(1): 5-20.
[37] Hibberd J M, Quick W P, Press M C, et al. The influence of the parasitic angiosperm Striga gesnerioides on the growth and photosynthesis of its host, Vigna unguiculata. Journal of Experimental Botany, 1996, 47(4): 507-512.
[38] Saikkonen K, Young C A, Helander M, et al. Endophytic Epichloё species and their grass hosts: From evolution to applications. Plant Molecular Biology, 2016, 90(6): 665-675.
[39] Song M L, Li X Z, Saikkonen K, et al. An asexual Epichloё endophyte enhances waterlogging tolerance of Hordeum brevisubulatum. Fungal Ecology, 2015, 13: 44-52.
[40] Saikkonen K, Lehtonen P, Helander M, et al. Model systems in ecology: Dissecting the endophyte-grass literature. Trends in Plant Science, 2006, 11(9): 428-433.
[41] Saikkonen K, Faeth S H, Helander M, et al. Fungal endophytes: A continuum of interactions with host plants. Annual Review of Ecology and Systematics, 1998, 29(1): 319-343.
[42] Tomilov A A, Tomilova N B, Abdallah I, et al. Localized hormone fluxes and early haustorium development in the hemiparasitic plant Triphysaria versicolor. Plant Physiology, 2005, 138(3): 1469-1480.
[43] Bar-Nun N, Sachs T, Mayer A M. A role for IAA in the infection of Arabidopsis thaliana by Orobanche aegyptiaca. Annals of Botany, 2007, 101(2): 261-265.
[44] De Battista J, Bacon C, Severson R, et al. Indole acetic acid production by the fungal endophyte of tall fescue. Agronomy Journal, 1990, 82(5): 878-880.
[45] Vadassery J, Ritter C, Venus Y, et al. The role of auxins and cytokinins in the mutualistic interaction between Arabidopsis and Piriformospora indica. Molecular Plant-Microbe Interactions, 2008, 21(10): 1371-1383.
[46] Schmid J, Day R, Zhang N, et al. Host tissue environment directs activities of an Epichloё endophyte, while it induces systemic hormone and defense responses in its native perennial ryegrass host. Molecular Plant-Microbe Interactions, 2017, 30(2): 138-149.
[47] Taylor A, Martin J, Seel W E. Physiology of the parasitic association between maize and witchweed (Striga hermonthica): Is ABA involved? Journal of Experimental Botany, 1996, 47(8): 1057-1065.
[48] Cheng X, Floková K, Bouwmeester H, et al. The role of endogenous strigolactones and their interaction with ABA during the infection process of the parasitic weed Phelipanche ramosa in tomato plants. Frontiers in Plant Science, 2017, 8: 392.
[49] Scott B, Green K, Berry D. The fine balance between mutualism and antagonism in the Epichloё festucae-grass symbiotic interaction. Current Opinion in Plant Biology, 2018, 44: 32-38.
[50] Faeth S H, Fagan W F. Fungal endophytes: Common host plant symbionts but uncommon mutualists. Integrative and Comparative Biology, 2002, 42(2): 360-368.

不同密度甘肃马先蒿寄生和内生真菌互作对紫花针茅内源激素及生物碱含量的影响

Interactive effects of different densities of Pedicularis kansuensis parasitism and Epichloё endophyte infection on the endogenous hormone levels and alkaloid contents of Stipa purpurea

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	张小芳, 魏小红, 刘放, 朱雪妹. PEG胁迫下紫花苜蓿幼苗内源激素对NO的响应[J]. 草业学报, 2021, 30(4): 160-169.
[2]	陈雅琦, 苏楷淇, 陈泰祥, 李春杰. 混合盐碱胁迫对醉马草种子萌发及幼苗生理特性的影响[J]. 草业学报, 2021, 30(3): 137-157.
[3]	李冬, 申洪涛, 王艳芳, 王悦华, 王丽君, 赵世民, 刘领. 外源褪黑素对干旱胁迫下烟草幼苗光合碳同化和内源激素的影响[J]. 草业学报, 2021, 30(1): 130-139.
[4]	崔雪莲, 夏超. 外源脱落酸对醉马草内生真菌共生体幼苗建植过程的影响[J]. 草业学报, 2020, 29(7): 70-80.
[5]	刘燕, 于美玲, 张然, 牛奎举, 李玉珠, 张金青, 马晖玲. 甘肃野生草地早熟禾内源激素含量的变化与无融合生殖率的关系研究[J]. 草业学报, 2020, 29(7): 99-111.
[6]	李秀璋, 宋辉, 张宗豪, 徐海峰, 刘欣, 李玉玲, 李春杰. 甘肃内生真菌基因组密码子使用的偏好性分析[J]. 草业学报, 2020, 29(5): 67-77.
[7]	康彩睿, 谢军红, 李玲玲, 王嘉男, 郭喜军, 彭正凯, 王进斌, Setor kwami Fudjoe, 王林林. 种植密度与施氮量对陇中旱农区玉米产量及光合特性的影响[J]. 草业学报, 2020, 29(5): 141-149.
[8]	李柯, 施宠, 何飞焱, 李昊宇. Pb胁迫下内生真菌侵染对德兰臭草生长及生理的影响[J]. 草业学报, 2020, 29(3): 112-120.
[9]	何雅丽, 陈振江, 魏学凯, 张海娟, 刘阳, 刘辉, 李春杰. 喷施茉莉酮酸甲酯及感染内生真菌促进醉马草抗虫性的生理作用研究[J]. 草业学报, 2020, 29(3): 121-129.
[10]	衣琨, 赵一航, 胡尧, 刘佳雪, 贺涛涛, 李旭, 宋鹏, 崔国文, 殷秀杰. GA₃和6-BA对高加索三叶草根蘖芽生长及内源激素含量的影响[J]. 草业学报, 2020, 29(2): 22-30.
[11]	鲍根生, 宋梅玲, 王玉琴, 李春杰. 甘肃马先蒿寄生对禾草内生真菌共生体共生关系的影响[J]. 草业学报, 2020, 29(2): 42-51.
[12]	王宁, 付亚军, 袁美丽, 刘征阳, 张铭鑫, 米银法. GA₃浸种对入侵植物节节麦种子破眠及发芽特性的影响[J]. 草业学报, 2020, 29(2): 73-81.
[13]	马碧花, 蔺伟虎, 高敏, 王兴迪, 田沛. 干旱胁迫下水杨酸和内生真菌对多年生黑麦草的影响[J]. 草业学报, 2020, 29(1): 135-144.
[14]	鲍根生, 俞永飞, 李春杰. 高寒草甸优势禾草-异针茅内生真菌的分离与鉴定[J]. 草业学报, 2019, 28(8): 150-160.
[15]	程瑞希, 字洪标, 罗雪萍, 杨有芳, 代迪, 王艳丽, 所尔阿芝, 王长庭. 青海省森林林下草本层化学计量特征及其碳储量[J]. 草业学报, 2019, 28(7): 26-37.