Skip to main content
  • Poster presentation
  • Published:

Hepatic mitochondrial dysfunction in fluid-resuscitated porcine septic shock

Background

Sepsis-induced multiple organ failure may crucially depend on the development of mitochondrial dysfunction and consequent cellular energetic failure. We investigated whether hepatic mitochondrial dysfunction was present in a clinically relevant porcine model of fluid-resuscitated septic shock.

Methods

Anesthetized and ventilated pigs (40 ± 3 kg) were randomly assigned to septic shock by fecal peritonitis (F, n = 3) or control (C, n = 3) after placement of portal/hepatic vein catheters and portal vein and hepatic artery flow probes. F and C received 8 ± 13 ml/kg/hour and 5 ± 7 ml/kg/hour ringer lactate + starch, respectively. The mean arterial pressure (MAP), total liver flow (TLF), hepatic O2 delivery (DO2,h) and hepatic O2 consumption (VO2,h) were recorded at baseline (BL), 12 and 24 hours (ml/kg/min). Activities of mitochondrial respiratory chain enzymes (complex I–IV) were assessed by spectrophotometry in snap-frozen liver samples. Data are presented as the mean ± SD.

Results

Hyperdynamic circulation developed in F with increasing DO2,h and decreasing VO2,h (Table 1). Complex II activity significantly decreased from 19.3 ± 4.2 to 9.5 ± 2.6 (P < 0.05 vs BL and between groups) in F compared with C. Complex I–III–IV function decreased in parallel in F.

Table 1 abstract P258

Conclusion

While increasing DO2,h far exceeded decreasing VO2,h in the setting of hyperdynamic fluid-resuscitated septic shock, hepatic mitochondrial function was significantly impaired compared with control.

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wauters, J., Vanhorebeek, I., Dieudonne, A. et al. Hepatic mitochondrial dysfunction in fluid-resuscitated porcine septic shock. Crit Care 11 (Suppl 2), P259 (2007). https://0-doi-org.brum.beds.ac.uk/10.1186/cc5419

Download citation

  • Published:

  • DOI: https://0-doi-org.brum.beds.ac.uk/10.1186/cc5419

Keywords