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Prevalence of nasal colonisation by methicillin-sensitive and methicillin-resistant Staphylococcus aureus among healthcare workers and students in Madagascar

BMC Infectious Diseases volume 16, Article number: 420 (2016) Cite this article

Abstract

Background

Methicillin-resistant Staphylococcus aureus (MRSA) clones pose a significant threat to hospitalised patients because the bacteria can be transmitted by asymptomatic carriers within healthcare facilities. To date, nothing is known about the prevalence of S. aureus and MRSA among healthcare workers in Madagascar.

The objective of our study was to examine the prevalence and clonal epidemiology of nasal S. aureus and MRSA among healthcare workers and non-medical University students in Antananarivo, Madagascar.

Methods

This cross sectional study screened nasal swabs taken from students and healthcare workers for S. aureus. Multiplex PCR was performed to identify S. aureus-specific (nuc), MRSA-specific mecA and mecC genes, Panton-Valentine leukocidin (PVL) (lukF-PV), and toxic shock syndrome toxin-1 (TSST-1) specific genes in methicillin-sensitive S. aureus (MSSA) and MRSA isolates. Staphylococcus protein A gene (spa) typing was performed for all confirmed MRSA isolates. The frequency distribution of nasal S. aureus and MRSA of healthcare workers and non-medical students was compared using Pearson’s χ2 test.

Results

Of 1548 nasal swabs tested, 171 (11 %) were positive for S. aureus; 20 (1.3 %) of these isolates were identified as MRSA. S. aureus was detected in 91 of 863 healthcare workers (10.4 %) and in 80 (11.8 %) of 685 students; however, 14 (1.5 %) healthcare workers carried MRSA compared with six (0.9 %) students. Nasal carriage of S. aureus and MRSA was more prevalent in women than in men, and 21 (11.7 %) S. aureus isolates were PVL-positive and 36 (21 %) were TSST-1 positive. The mecC gene was not detected in any isolates. Five different spa types were identified, with spa type t186 being the predominant MRSA clone (16/20).

Conclusion

The results of the present study reveal a low frequency of S. aureus and MRSA nasal carriage in both students and healthcare workers from Antananarivo, Madagascar. The predominant MRSA clone (t186) was previously described in hospitalised patients in Madagascar.

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Background

The bacterium Staphylococcus aureus causes a variety of infections, ranging from skin and soft tissue infections to life-threatening disease [1]. It is also a commensal that colonises the anterior nares of asymptomatic carriers, who may unknowingly transmit the pathogen within the community or within healthcare facilities [2]. Carriage among healthcare workers (HCW) may act as a source of infection in hospitals [3]. Since the introduction of β-lactam antibiotics, the spread of methicillin-resistant S. aureus (MRSA) has increased on a global scale [4, 5].

Data regarding the prevalence and distribution of methicillin-sensitive S. aureus (MSSA) and MRSA in Africa are scarce, and control measures within healthcare settings are limited due to constraints with respect to resources and diagnostic facilities [6]. Studies on S. aureus carriage by hospital patients in Madagascar report a MRSA prevalence of between 4 and 13 % [7–11]. The prevalence of S. aureus nasal carriage in outpatients was 38, and 15 % of cases were MRSA [7]. Madagascan hospitals set no guidelines for screening patients or HCW for MRSA.

Different MRSA strains have emerged in hospitals and in the community in both Europe and the USA [12, 13]. Factors such as travel and close contact with animals may contribute to the dissemination of the bacterium in Africa: one MRSA clone in particular (ST-88-MRSA), which is found worldwide, is also widespread in West, Central, and East Africa [14]. A multicentre study conducted across five different African countries identified one major clone, which is also predominant in Madagascar (ST-88 IV, spa type t186) [15]. A high prevalence of the staphylococcal virulence factor, Panton-Valentine leukocidin (PVL), was found in MSSA strains isolated from hospital patients from mainland Africa and the Western Indian Ocean region [8, 16, 17]. Evidence suggests that PVL is associated with staphylococcal skin and soft tissue infections [18]. No data are available on the presence of PVL in MSSA and MRSA isolates obtained from HCW in Madagascar. Another staphylococcal virulence factor, toxic shock syndrome toxin-1 (TSST-1), causes severe illness and multisystem clinical manifestations [19]. Few studies report the prevalence of TSST-1 in healthy carriers from Africa; to date, no data are available with respect to Madagascan HCW [20, 21]. Resistance to β-lactam antibiotics (as in MRSA) is due to the acquisition of the mecA gene. In recent years, a similar gene termed mecC has been described, which is often not detected by routine laboratory tests because standard molecular methods usually focus only on detecting mecA [22].

Here, we obtained nasal swabs from HCW from different hospitals and from healthy non-medical students at the University of Antananarivo. The aim was to identify the prevalence and local clonal epidemiology of S. aureus and MRSA in Madagascar.

Methods

Study design

This cross sectional study enrolled non-medical students from the University of Antananarivo and HCW from five different hospitals (University Children’s Hospital, the surgery department at the University Gynaecological Hospital, the University Infectious Diseases department, a military hospital, and a private clinic for general and trauma surgery) and one dispensary in Antananarivo, Madagascar. HCW were defined according to the WHO definition [23], which included all study participants working in a hospital. Hospital staff were informed and recruited during in-house training sessions, while university students were asked to participate during seminars and lectures.

Sample size calculation

The required sample size to yield at least k = 5 MRSA isolates with about 90 % probability was estimated by assuming that the proportion of MRSA among S. aureus isolates was 5 %, and that 35 % of all sampled individuals were carrying S. aureus. Using the formula

$$ 1-{\displaystyle \sum_{i=0}^k\left({}_k^n\right)}{p}^k{\left(1-p\right)}^{n-k} $$

the required minimum sample size was estimated at 526 per arm (HCW and non-medical university students).

Data and sample collection procedures

After providing informed consent, demographic data (e.g., sex, age, home community) was collected via a questionnaire. Students were asked to name their faculty, and hospital employees were asked to describe their role and whether their work included regular contact with patients. Respondents were also asked about hospital admissions within the last 6 months, their history of antimicrobial treatment within the last 6 months, whether they lived in a student/healthcare staff hall, whether they nursed chronically ill relatives at home, whether they suffer from a chronic illness, and whether they had any acute skin diseases such as atopic dermatitis, psoriasis or chronic ulcers. As S. aureus colonises pets and livestock, the participants were asked whether they had contact with animals [14]. After the participants filled in the questionnaire, they were instructed on how to collect a self-administered nasal swab.

Microbiological examination of nasal swabs in Madagascar

The study team demonstrated the technique used to obtain nasal samples using a sterile cotton-tipped swab (Copan Nasal Swab Transsystem®, Brescia, Italy) and provided assistance where necessary. The samples were then immediately transported to the study laboratory where bacteria were isolated as described below.

After incubation on Columbia blood agar (OXOID®, Vienna, Austria) for 18–24 h at 35–37 °C, the first step of the identification procedure involved examination of colony morphology and Gram staining. Suspected S. aureus colonies on the blood agar plate were tested for catalase and coagulase activity, and for latex agglutination (Staphytec plus, OXOID®) [24]. S. aureus strains were tested for antibiotic susceptibility using the standard disc diffusion method on Mueller-Hinton Agar, according to current CLSI (Clinical and Laboratory Standards Institute) guidelines. Antibiotic susceptibility testing was done for the following locally available and prescribed antibiotics: penicillin, erythromycin, clindamycin, cotrimoxazole, and ampicillin/sulbactam. Cefoxitin was used as an MRSA screening agent.

S. aureus ATCC-25923, BAA 9176, and methicillin-resistant S. aureus ATCC-43300 were used as controls. S. aureus isolates were stored at −80 °C in Cryobank tubes (Cryobank, MAST®, Liverpool, UK) until shipment at -20 °C to the Bernhard Nocht Institute for Tropical Medicine, Germany, for molecular analyses. The QIAamp DNA Mini Kit (Qiagen®, Hilden, Germany) was used to extract DNA from all S. aureus isolates, according to the manufacturer’s instructions.

Multiplex PCR to confirm S. aureus isolates

In Germany, all S. aureus isolates were further screened for S. aureus-specific (nuc), MRSA-specific (both mecA and mecC), and PVL and TSST-1 virulence genes by multiplex PCR. The primers used are listed in Additional file 1: Supporting Information Table S1.

PCR was performed using the Qiagen® multiplex PCR kit (ref. no.-201643). Only strains harbouring the mecA or mecC genes were classified as MRSA [25].

Staphylococcus protein A gene (spa) typing of MRSA isolates

To examine the clonal epidemiology of MRSA, all PCR-confirmed MRSA isolates were subjected to spa typing using the RidomStaphType standard protocol, followed by double-strand sequencing [26]. Sequences were analysed using RidomStaphType version 2.2.1 software (Ridom Ltd., Würzburg, Germany). Associated multi-locus sequence types (MLST) were automatically allocated by the Ridom SpaServer according to the based-upon-repeat-pattern (BURP) algorithm (if sufficient data were available) [27], or retrieved from the literature.

Treatment of MRSA-positive individuals

If the nasal swabs tested positive for MRSA in Madagascar, the participant was offered treatment with Mupirocin (Turixin®) ointment to eradicate the nasal MRSA (treatment followed the guidelines set down by the Robert-Koch-Institute, Germany) [28]. After treatment, further nasal swabs were taken to confirm successful decolonisation.

Statistical analyses

Pearson’s χ2 test or Fisher’s exact test was used to compare the frequency distribution of S. aureus and MRSA nasal carriage by HCW and non-medical students. Age, sex, occupation, and previous antimicrobial use were variables entered into a multivariable logistic regression model to assess their adjusted association with S. aureus carriage. Analyses were performed using Stata 12 (StataCorp LP, College Station, USA) software.

Results

Nasal swabs were taken from 863 HCW and 685 students. S. aureus was identified in 171 isolates (11.0 %), of which 20 (1.3 %) were MRSA (Fig. 1).

Fig. 1
figure 1

Examination procedure for nasal swabs obtained from university students and healthcare workers. In a first step S.aureus were identified via PCR-screening for nuc genes and in a second step these S.aureus were screened for mecA and mecC genes allowing to differentiate between methicillin-resistant and methicillin-sensitive Staphylococcus aureus

Full size image

The point prevalence of nasal S. aureus carriage was 91 (10.4 %) in the HCW group and 80 (11.4 %) in the student group. MRSA carriage was slightly higher in the HCW group (1.5 %) than in the student group (0.9 %).

Of the MSSA isolates, 7.3 % were susceptible to penicillin (Table 1). Erythromycin-induced resistance to clindamycin was not detected in any of the MSSA or MRSA isolates.

Table 1 Antibiotic susceptibility of methicillin-sensitive and methicillin-resistant Staphylococcus aureus
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The prevalence of MRSA carriage was equal among groups of the same age, and increased with age (Table 2). Overall, nasal carriage of S. aureus was more common in women (12.7 %) than in men (8.0 %). Carriage of S. aureus and MRSA was slightly higher in HCW that reported direct patient contact (Table 3). S. aureus and MRSA carriage stratified according to medical profession or workplace is shown in Table 3. There was no strong association between MRSA carriage and other risk factors (Tables 4 and 5). Multivariable logistic regression analyses identified female gender and age > 25 years as significantly associated with nasal carriage of S. aureus (p = 0.003 and p = 0.04, respectively) (Table 6).

Table 2 Prevalence of Staphylococcus aureus (S. aureus) and methicillin-sensitive (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) in healthcare workers and students in Antananarivo, Madagascar (N = 1548)
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Table 3 Descriptive characteristics of healthcare workers from Antananarivo, Madagascar carrying Staphylococcus aureus (S. aureus) and methicillin-resistant Staphylococcus aureus (MRSA)
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Table 4 Descriptive characteristics of healthcare workers from Antananarivo, Madagascar, with respect to carriage of Staphylococcus aureus (S. aureus) and methicillin-resistant Staphylococcus aureus (MRSA)
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Table 5 Descriptive characteristics of students from Antananarivo, Madagascar, with respect to carriage of Staphylococcus aureus (S. aureus) and methicillin-resistant Staphylococcus aureus (MRSA)
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Table 6 Multivariable logistic regression analyses of factors associated with nasal colonisation of healthcare workers and university students by Staphylococcus aureus
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In total, 21 S. aureus isolates (11.7 %) harboured PVL and 36 (21.1 %) harboured TSST-1. Ten (1.2 %) HCW and 11 (1.6 %) students carried PVL-positive strains in the anterior nares, while 20 HCW (2.3 %) and 16 (1.9 %) students carried TSST-1-positive strains (Table 7).

Table 7 Molecular epidemiology of methicillin-resistant Staphylococcus aureus isolated from students and healthcare workers from Antananarivo (n = 20)
Full size table

Recent findings indicate global occurrence of a new divergent mecA homologue, named mecC [29]; therefore, all samples were also screened for the mecC gene. All MRSA isolates identified in this study harboured the mecA gene, but not the mecC gene.

Staphylococcus protein A gene (spa) typing was performed for all 20 MRSA isolates. Of the five different spa types identified, spa type t186 was the predominant clone (16/20, 80.0 %) among both HCW and students (Table 7). The associated sequence type (ST) for spa types t186 and t2393 was ST-88. Eighteen MRSA-positive participants were treated with Mupirocin and were successfully decolonised. In accordance with the current guidelines of the Robert-Koch-Institute, Germany, one HCW required repeated Mupirocin treatment to ensure eradication [28].

Discussion

To the best of our knowledge, this is the first study to report nasal S. aureus and MRSA carriage rates in HCW and non-medical university students in Madagascar. The overall prevalence of nasal S. aureus carriage identified in this study is lower than that reported in another study from Madagascar, which identified MSSA in 38 % of outpatients [7].

The finding that nasal carriage of S. aureus is higher in females is in line with a study of HCW from Norway, which showed that female HCW had a 54 % increased risk of S. aureus nasal carriage versus non-HCW (OR, 1.54; 95 % CI, 1.09–2.19). However, the Norwegian study differed from the current study in terms of both study design and detection methods used [30].

Data regarding nasal carriage of MRSA by African HCW are scarce. Studies of HCW from Ethiopia and Libya identified nasal carriage rates for MRSA of 14.1 % [31] and 36.8 % [32]), respectively, whereas a study from Kenya found a nasal carriage rate of 0 % [33]. A study from São Tomé and Principe sampled HCW at two different time points and found that the MRSA prevalence ranged from 1.6 to 4.0 % [34]. A systematic review of MRSA carriage by HCW in non-outbreak situations in Europe and the USA calculated a pooled MRSA colonisation rate of 1.8 % (95 % CI, 1.34–2.50 %) [35]. Carriage rates identified by the individual studies in this particular review ranged from 0.2 to 15 %. Taken together, these studies show that MRSA carriage rates in HCW are highly variable.

PVL is a cytotoxin that causes dermonecrosis and lysis of human granulocytes and increases the adherence of S. aureus to the extracellular matrix [36]. Evidence suggests that PVL is associated with staphylococcal skin and soft tissue infections and with severe necrotising pneumonia [18]. As MRSA-colonised HCW may act as a source of infection in outbreak settings [3], we sought to identify this virulence factor in all S. aureus isolates. Previous studies from Africa reported high rates for PVL in MSSA strains (ranging from 17 to 74 %) [8, 16]. A study from Gabon reported high PVL rates in both clinical isolates (54.7 %) and isolates obtained from asymptomatic carriers (40.5 %) [21]. Finally, a study by Breurec et al. found that 30 % of clinical isolates from Antananarivo were PVL-positive [8]. The comparatively moderate PVL-positivity rate (11.6 %) identified in our study is in contrast with that found in industrialised countries, in which PVL is rare; however, our findings support those of previous studies from sub-Saharan Africa [8, 16, 21].

A recent study from Congo identified the TSST-1-encoding gene in 17.5 % of all S. aureus isolates obtained from HCW [20]. We found even higher rates in both groups examined herein, suggesting that TSST-1 may be more prevalent in S. aureus strains from Africa than in strains from Europe, making infection control practices in Malagasy hospitals an urgent priority.

MRSA spa type t186 was the predominant clone identified in the current cohort, and the most common type identified in HCW. Spa type t186 was one of the clones previously circulating in hospital patients in Antananarivo, Madagascar [15]. According to the RidomSpa Server database [26], this clone occurs worldwide, and the automatically allocated sequence type ST-88 [27] is predominant in West, Central and East Africa [14]. The spa type t2393 identified herein was also identified in hospital isolates from Ghana, north-eastern Argentina and from a patient from Berlin with a travel history to Panama, whereas spa type t5562 was found in New Zealand isolates [37–40].

Limitations

A lack of selective enrichment before culture on agar plates may explain the low prevalence of S. aureus detection. Also, S. aureus frequently colonises other sites on the skin, which were not swabbed in this study due to ethical and operational reasons [41]. This may also account for the low rate of S. aureus detection.

The samples examined herein were collected by self-administered nasal swabbing; therefore, sampling may have not been carried out in the best possible way. However, other large, population-based studies of MRSA carriage in Europe were based on self-swabbing techniques, and nasal self-swabbing itself has been shown to be appropriate for the detection of S. aureus and MRSA [42–44].

A large part of our study population consisted of students; therefore, we may expect a lower prevalence of S. aureus in such healthy participants compared with that in outpatients or hospitalised patients.

It was our intention not to include high-risk groups such as children or people over the age of 69 years, and a group of healthy and relatively young students was used as a proxy for community controls. The relatively low number of S. aureus and MRSA isolates mean that the present study has low statistical power; therefore, we may not have detected any association between colonisation and risk factors such as skin lesions [3].

Conclusion

In conclusion, this study provides a good overview of MRSA clones circulating in this particular region of Madagascar. The prevalence of MRSA in HCW and healthy students from Madagascar was low, and all S. aureus strains showed high resistance to penicillin, which is in line with other reports across the African continent. Spa type t186, which is the dominant clone on mainland Africa, was also the predominant MRSA clone identified in the current study population. The fairly high rates of TSST-1 and the presence of PVL-producing strains represent a potential reservoir and source for possible severe infection, which could lead to the emergence of successful MRSA clones. From a clinical point-of-view, MRSA screening programs in Africa are limited by a lack of facilities and a lack of qualified staff and financial resources. Antibiotic regimens to eradicate MRSA are expensive. However, general hygiene measures (e.g., hand washing) are both cheap and effective at preventing the spread of MRSA by HCW and should be promoted and performed diligently.

Abbreviations

BURP, based-upon-repeat-pattern; CC, clonal complex; CLSI, Clinical and Laboratory Standards Institute; HCW, Healthcare workers; MLST, multi-locus sequence type; MRSA, methicillin-resistant Staphylococcus aureus; MSSA, methicillin-sensitive Staphylococcus aureus; PVL, Panton-Valentine leukocidin; S. aureus, Staphylococcus aureus; spa typing, Staphylococcus protein A gene typing; ST, sequence type; TSST, toxic shock syndrome toxin

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Acknowledgments

We are grateful to Auguste Rakotondramanana and Jean de Dieu H. Rafalimanana for technical assistance and to Dr. Daniel Eibach for comments about the manuscript. We also thank all the volunteers for participating in this study.

Funding

This study was co-funded by the Else Kröner-Fresenius-Stiftung. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Availability of data and materials

Materials and data can be shared upon request through the corresponding author.

Authors’ contributions

Analysed the data: BH, DD, and NGS. Contributed reagents/materials/analysis tools: RR, SP, HF, RMH, VM, SC, DD, SC, and JM. Wrote the paper: BH, DD, NGS, SP, HF, RMH, RR, and JM. Designed the study: NGS, JM, BH, RR, DD, AH, AJ, SP, HF, and RMH. Conducted and supervised field work: BH, DD, RR, JPR, and TR. Conducted laboratory work: DD, BH, HAE, VM, and SC. All authors read and approved the final manuscript.

Competing interests

The authors declare that they have no competing interests.

Consent for publication

Not applicable.

Ethics approval and consent to participate

The study was approved by the Ethical Committee of the Ministry of Health of the Republic of Madagascar (no. 081 – MSANP/CE, 5 November 2012). Healthy volunteers were informed about the study protocol in both French and Malagasy, with an information sheet written in French. The aims of the study were also explained by an investigator (in both French and Malagasy) to small groups of people before each person provided written informed consent. The laboratory study team was blinded to the names of the participants. The study investigators were the only persons allowed to inform and counsel the volunteers after the results were obtained.

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Author notes

    Authors and Affiliations

    1. Research Group Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine (BNITM), Bernhard-Nocht-Str. 74, D-20359, Hamburg, Germany

      Benedikt Hogan, Hassan Al-Emran, Denise Dekker, Andreas Hahn, Anna Jaeger, Jürgen May & Norbert Georg Schwarz

    2. Department of Microbiology and Parasitology, University of Antananarivo, B.P. 175, Antananarivo, Madagascar

      Raphael Rakotozandrindrainy, Jean Noel Heriniaina, Jean Philibert Rakotondrainiarivelo & Tsiriniaina Razafindrabe

    3. University Medical Center, Hamburg-Eppendorf, Martinistr. 52, 20251, Hamburg, Germany

      Sven Poppert

    4. Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Bernhard-Nocht-Str. 74, D-20359, Hamburg, Germany

      Hagen Frickmann, Ralf Matthias Hagen & Volker Micheel

    5. Institute for Medical Microbiology, Virology and Hygiene, University Hospital of Rostock, Schillingallee 70, D-18057, Rostock, Germany

      Hagen Frickmann & Sabine Crusius

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    1. Benedikt Hogan
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    Corresponding author

    Correspondence to Benedikt Hogan.

    Additional file

    Additional file 1:

    Supporting Information Table S1. Primers used for PCR screening of nasal S. aureus isolates obtained from Madagascan students and healthcare workers. (PDF 73 kb)

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    Hogan, B., Rakotozandrindrainy, R., Al-Emran, H. et al. Prevalence of nasal colonisation by methicillin-sensitive and methicillin-resistant Staphylococcus aureus among healthcare workers and students in Madagascar. BMC Infect Dis 16, 420 (2016). https://0-doi-org.brum.beds.ac.uk/10.1186/s12879-016-1733-6

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    Keywords

    BMC Infectious Diseases

    ISSN: 1471-2334

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