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Counteracting Age-related Loss of Skeletal Muscle Mass: a clinical and ethnological trial on the role of protein supplementation and training load (CALM Intervention Study): study protocol for a randomized controlled trial

Abstract

Background

Aging is associated with decreased muscle mass and functional capacity, which in turn decrease quality of life. The number of citizens over the age of 65 years in the Western world will increase by 50 % over the next four decades, and this demographic shift brings forth new challenges at both societal and individual levels. Only a few longitudinal studies have been reported, but whey protein supplementation seems to improve muscle mass and function, and its combination with heavy strength training appears even more effective. However, heavy resistance training may reduce adherence to training, thereby attenuating the overall benefits of training. We hypothesize that light load resistance training is more efficient when both adherence and physical improvement are considered longitudinally. We launched the interdisciplinary project on Counteracting Age-related Loss of Skeletal Muscle Mass (CALM) to investigate the impact of lifestyle changes on physical and functional outcomes as well as everyday practices and habits in a qualitative context.

Methods

We will randomize 205 participants older than 65 years to be given 1 year of two daily nutrient supplements with 10 g of sucrose and 20 g of either collagen protein, carbohydrates, or whey. Further, two groups will perform either heavy progressive resistance training or light load training on top of the whey supplement.

Discussion

The primary outcome of the CALM Intervention Study is the change in thigh cross-sectional area. Moreover, we will evaluate changes in physical performance, muscle fiber type and acute anabolic response to whey protein ingestion, sensory adaptation, gut microbiome, and a range of other measures, combined with questionnaires on life quality and qualitative interviews with selected subjects. The CALM Intervention Study will generate scientific evidence and recommendations to counteract age-related loss of skeletal muscle mass in elderly individuals.

Trial registration

ClinicalTrials.gov NCT02034760. Registered on 10 January 2014.

ClinicalTrials.gov NCT02115698. Registered on 14 April 2014.

Danish regional committee of the Capital Region H-4-2013-070. Registered on 4 July 2013.

Danish Data Protection Agency 2012-58-0004 – BBH-2015-001 I-Suite 03432. Registered on 9 January 2015.

Peer Review reports

Background

On the basis of demographic extrapolations, the number of elderly citizens above the age of 65 years will increase in the next three decades by 50–200 %, with the specific proportion being dependent on country [1, 2]. With biological aging, physical function often decreases. This increases the risk of developing frailty [3], indicating higher risk for adverse events. Even in healthy, active, and independently living older people, a gradual loss of muscle mass, termed sarcopenia [4], takes place at an annual rate of up to 1–2 % starting in the sixth decade of life [57]. The loss of muscle mass is accompanied by an even faster deterioration of muscle strength of up to 3.5 % per year [8]. If allowed to progress, the development of sarcopenia is associated with increased risk of falling [9], decreased satisfaction with life [10], and even an increased mortality rate [11]. The impact of sarcopenic progression may become detrimental to an individual’s personal life and autonomy, and the societal implications are vast when one considers future healthcare and nursing expenditures. Therefore, in the present study, we aim to test the efficacy and feasibility of different strategies for counteracting muscular deterioration. Preferably, these strategies should be easy to integrate into everyday life for the majority of the aging population to induce maximal efficiency at both individual and societal levels.

Previous research has described the multifactorial nature of the development of sarcopenia [1214]. On the basis of the aging muscle becoming less sensitive to daily anabolic stimuli due to protein intake [15] and muscular activity [16, 17], it is suggested that exactly these two factors possess a high potential to antagonize sarcopenia. Further, the nutrition and exercise training strategies are self-manageable, allowing a certain degree of flexibility for adjustment to personal preferences and everyday practices. Although their mutual dependency is acknowledged [18, 19], the “dosing” of each factor remains questionable, and the impact during long-term exposure is unknown. In contrast to studies of strategies used for treating individuals who already have loss of muscle mass and function, the aim of the Counteracting Age-related Loss of Skeletal Muscle Mass (CALM) Intervention Study is to evaluate strategies to prevent sarcopenic progression in healthy, independently living aging individuals.

With regard to protein intake, cohort studies strongly suggest an association between high protein intake and decreased rates of age-dependent decline in physical performance and reduced risk of frailty [2022]. However, researchers in intervention studies have reported inconclusive effects on physical function and muscle mass after administering protein supplements for longer periods [2325]. Research has shown that ingestion of 10 g of essential amino acids [26], corresponding to roughly 20 g of whey protein or even 35–40 g of dairy protein at rest [27] or after exercise [28], can stimulate muscle protein synthesis (MPS) fully. The quality of the ingested protein is a matter of concern, and the protein digestibility-corrected amino acid score (PDCAAS) [29] is a way to evaluate this. Protein amino acid composition and protein digestibility are the two notable factors that determine the PDCAAS, but using hydrolyzed protein maximizes protein digestibility, especially for proteins with slow digestibility, such as casein, leaving amino acid composition as the most important factor for the protein of choice when investigating the effect on muscle mass. Research has shown that whey protein appears advantageous in comparison with other protein sources [30, 31]. While the focus has been mainly on protein type and quantity, experimental results combined with eating patterns of elderly persons recently led to the hypothesis that intake distribution may be of importance [32]. In Denmark, calculations suggest that citizens between 65 and 75 years of age consume a mean of 1.1 g of protein per kilogram of body weight per day [33, 34], indicating that the average senior citizen consumes a reasonable amount of protein [13, 3537]. This amount is well above the present recommended dietary allowance of 0.8 g/kg body weight [29, 38], which is based on the estimated average requirements ±2 SD. The pattern of protein ingestion is not currently mentioned in recommendations, and data derived from elderly citizens in the United States [39] and Denmark (Fagt S, Danskernes Kostvaner 2011-2013, unpublished data) demonstrate that almost half of the daily amount of protein is ingested at dinner. The implications of this may be that the MPS is maximally stimulated only once daily [27, 40, 41], despite daily average protein consumption appearing to be adequate. Therefore, we hypothesize that a recommendation for enhanced daily protein intake should include a guideline for an evenly distributed protein intake throughout the day to optimize muscle protein accretion [42, 43]. Promoting a healthier eating pattern may be successful in improving food choices, but this has a modest success in terms of actual lifestyle changes [44]. Therefore, if research should convert any new knowledge on the timely provision of additional protein intake into actual changes in daily eating patterns and life routines in the elderly target group, scientists must take into account the short-term (weeks) and long-term (months) acceptability of daily ingestion of food supplements. Additionally, to describe the impact of daily supplementation with protein on general health, researchers should also investigate additional benefits or compromising side effects of the protein intake.

Heavy load muscle training is undoubtedly the most effective exercise strategy to gain muscle mass and strength [4548], and even very old individuals can benefit from training by experiencing an increase in muscle mass and strength [18, 19, 4952]. However, at a societal level, the overall impact of implementing a certain training regime reflects a balance between the stimulatory effect of exercise training per se and tolerability and adherence to the training modality and setting. Activities of more moderate intensity, preferably those that are simple, convenient, and cheap [53], are associated with increased participation among the elderly. Further, these activities should be performed in an informal setting [54], and sessions in a training program seeking high adherence should be either home-based or conducted in smaller groups with peers [55, 56]. Therefore, despite an increased training effect per se, the feasibility of heavy load strength training requiring admittance to a fitness center or similar facility may be questionable for the elderly population [57]. As demonstrated in Fig. 1, home-based light load resistance training may prove to be a better recommendation for preventing age-related decreases in muscle mass and physical function because of increased adherence, but longitudinal research in the elderly is lacking.

Fig. 1
figure 1

Hypothesized improvements over time with different interventions. Black lines mark the expected effect of per-protocol analysis: HRTW (solid line), LITW (long-dashed line), WHEY (short-dashed line), COLL (dashed-dotted line), and CARB (dotted line) interventions when analyzed per protocol. The gray line marks the expected effect intention-to-treat analysis of HRTW. CARB carbohydrate supplementation group, COLL collagen supplementation group, HRTW heavy resistance training with whey supplementation, LITW light-intensity training with whey supplementation, WHEY whey supplementation

We have launched a 1-year longitudinal randomized controlled trial (RCT), partly single-blinded/partly double-blinded, to investigate the impact of protein quality and training intensity on different variables. The primary outcome measure is the change in knee extensor skeletal muscle cross-sectional area (CSA) over the course of 1 year of intervention. The secondary and tertiary outcomes are listed in the Measurements section below. In this RCT, we are integrating the evaluation of the interventions’ impact on general health, physical and functional parameters, and impact on daily life routines and practices as well as on the acceptability of the interventions, covering the entire spectrum from objective physiological measures to subjective investigations on the participants. All results will be fed into the integrative and interdisciplinary outcomes of the CALM Intervention Study (Fig. 2).

Fig. 2
figure 2

Spectrum of outcome variables. In the interdisciplinary Counteracting Age-related Loss of Skeletal Muscle Mass Intervention Study, we are investigating the entire spectrum of possible impacts of the intervention by applying objective, quantitative measures of the body and subjective, qualitative investigations of the participants

Working hypotheses

We designed the CALM Intervention Study to investigate two overarching working hypotheses:

1. Whey protein hydrolysate supplementation is more efficient than collagen protein hydrolysate and carbohydrate in improving muscle size when administered to healthy elderly individuals.

2. With whey protein hydrolysate supplementation, the addition of center-based heavy resistance training is advantageous to home-based light load resistance training, and both of these regimens are superior to no training at all, in regard to improving muscle mass, overall health, and physiological parameters only with a per-protocol analysis. This will not be the case in regard to intention-to-treat analysis, where a markedly higher adherence is presumed in the home-based training group, leading to a similar or superior response compared to the center-based heavy resistance training group.

Methods/design

Overview

The CALM Intervention Study is a single-center, partly single- and partly double-blinded, multidisciplinary RCT. Collaborators are affiliated with (1) the Faculty of Science, Department of Food Science, (2) the Faculty of Humanities, SAXO Institute, and (3) the Faculty of Health and Medical Sciences, Department of Biomedical Sciences, all at the University of Copenhagen and the Institute of Sports Medicine Copenhagen, Bispebjerg Hospital, Copenhagen, Denmark. We wrote the protocol using the Standard Protocol Items: Recommendations for Interventional Trials (SPIRIT) guidelines for clinical trial protocols (http://www.spirit-statement.org/spirit-statement) and registered the protocol at ClinicalTrials.gov. Any changes will be reported there and to the local ethics committee.

Recruitment of participants

The CALM Intervention Study will include 205 participants living in the Greater Copenhagen area through local newspapers, magazines, radio programs, social media, and presentations at senior centers and public events. See the Statistics and power calculations section below for further explanation on recruitment requirements. Recruitment commenced in January 2014. Exclusion criteria are listed in Table 1.

Table 1 Exclusion criteria

Information and screening

Upon first contact, a researcher informs the possible participants briefly about the project and sends written information by mail or e-mail. If the candidate is still interested after having read the material, the staff member subsequently screens for exclusion criteria and invites the person to an information meeting after which participants give their written informed consent in accordance with Declaration of Helsinki II. The protocol has been approved by The Danish Regional Committees of the Capital Region on 4 July 2013 (number H-4-2013-070).

At the information meeting, a medical member of the research team delivers the information verbally followed by a health screening including blood pressure, blood samples, and heart auscultation to determine if the candidate can safely complete the interventions. Then, the participant performs a 30-second chair-stand test that we use for stratified randomization only.

Randomization

Following screening and health examinations, we enroll candidate participants and researchers who are not involved in interventions or tests that are sensitive to blinding randomize them using the open-source minimization program MinimPy 0.3 [58, 59]. To ensure even distribution of subjects throughout the study period, we decided to employ a stratified, biased coin minimization with 0.95 base probability and to use allocation ratios corresponding to the group sizes (see Statistics and power calculations section below). Participant stratification is based on a 30-second chair-stand test (<16 or ≥16) and on gender to ensure equal allocation to one of the five intervention groups and further to determine if the participant will participate in invasive acute mechanistic MPS trials (see Muscle protein synthesis section below), as demonstrated in Fig. 3.

Fig. 3
figure 3

Participant flow. Ntotal represents the expected number of inclusions in each group. Nacute represents the expected number of participants who will complete the measurements of fractional synthesis rate at 0 and 12 months. COLL Collagen supplementation, CARB Carbohydrate supplementation, WHEY Whey supplementation, LITW Light intensity resistance training and whey supplementation, HRTW Heavy resistance training and whey supplementation

Interventions

The five intervention groups comprise the two arms of the study. One arm concerns the importance of supplementation using the three supplementation-only groups: carbohydrate supplementation (CARB; 20 g maltodextrin + 10 g sucrose), collagen supplementation (COLL; 20 g bovine collagen protein hydrolysate + 10 g sucrose), and whey supplementation (WHEY; 20 g whey protein hydrolysate + 10 g sucrose). All supplements were developed, prepared, and individually packaged by Arla Foods Ingredients Group P/S, Viby J, Denmark. The other arm investigates the importance of training loads and includes all groups supplemented with the aforementioned whey supplements, either without training (WHEY; the same group used in the supplementation arm), with light load home-based resistance training four times weekly (LITW), or with center-based heavy resistance training three times weekly (HRTW). Table 2 describes the training protocols in detail. All subjects consume the supplements twice daily, namely in the morning and at midday, preferably just before or during meals to increase satiety, thereby avoiding excess intake of calories. Participants who exercise are encouraged to take one supplement immediately after each training session. We conduct all interventions and tests at Bispebjerg Hospital. To report the improvement in thigh strength in repetition maximums (RM), we measure the thigh 3 RM in the knee extensor machine used for heavy resistance training (Super Executive Line; TechnoGym, Cesena, Italy) using as few attempts as possible every third month. To relate to the commonly used 1 RM, we will convert the result to a 1-RM equivalent using the Brzycki formula [60]: 1 RM = weight lifted × [36/(37 − repetitions)].

Table 2 Exercise descriptions

Participants monitor their adherence to protein supplementation and their home-based exercise in hard-copy diaries, whereas the trainers register the training adherence in the HRTW group. We stress the importance of registering only the actual number of supplements taken and exercises performed.

Blinding

We achieve blinding by storing the randomization log file electronically without access for researchers performing the tests who could be affected by not being blinded. Specifically, blinded radiographers will perform magnetic resonance imaging (MRI) scans at the Department of Radiology, Bispebjerg-Frederiksberg Hospital, Copenhagen, Denmark. A blinded physiotherapist or physiologist will perform strength and functional assessments, and only one tester will assess each participant. Experienced staff not blinded to the intervention will perform dual-energy X-ray absorptiometry (DXA), muscle biopsies, anthropometry, and blood sampling. Blinded investigators will perform all analysis and interpretation of obtained data. We blind participants in supplementation-only groups, as the bags containing supplements are marked only by a code, and only relevant researchers can access the key file containing the explanation of the codes. For obvious reasons, we did not blind those performing exercises to intervention or supplement type, as stated in the study hypotheses.

Measurements

This section describes each measurement performed to evaluate the longitudinal effects of the chosen interventions. Table 3 displays the time points for each examination. At baseline, we test the peak isometric and isokinetic peak torques as well as leg extensor power in both legs to evaluate if a large side difference between thigh peak torques is associated with an improved or diminished response to the interventions.

Table 3 Time schedule of participant engagement

Primary outcome

A change in muscle CSA is a direct reflection of the intervention’s ability to restore muscle protein and a direct consequence of the net protein balance over time in the target muscles. We chose m. quadriceps and m. vastus lateralis CSA based on MRI scans of the dominant thigh as the primary outcome, as it directly reflects the intervention’s ability to counteract age-related loss of muscle mass. MRI scans have high sensitivity and validity in terms of lean mass in skeletal muscle [61], and previous researchers have considered this modality superior to DXA [62, 63].

Each scan consists of six axial slices, with the first slice placed in the medial tibia plateau. Each slide is 8 mm thick and separated by a 60-mm gap as shown in Fig. 4. The primary time interval for assessment is from baseline to 12 months.

Fig. 4
figure 4

Magnetic resonance imaging analysis. We place slices as shown for analysis of cross-sectional area of the m. quadriceps femoris muscle and analyze slices 3 (counting in distal to proximal direction) and 4 for all subjects, and we use slice 4 for primary outcome evaluation. We fix the placement of slices in absolute distances, but we measure the femur length on dual-energy X-ray absorptiometric scans from the lateral tibial plateau (0 %) to the top of the greater trochanter (100 %) to report the relative placement of slices. Currently, placement of slice 3 ranges from 27 % to 36 % and slice 4 from 40 % to 54 % of the femoral length, depending on the height of the participant

Secondary outcomes

Thigh isometric peak torque

After having the participant perform a brief warm-up on a cycle ergometer, a tester will measure the dominant thigh peak strength at 70-degree flexion (0 degrees represents horizontal) in a Kinetic Communicator (model 500-11, Kinetic Communicator; Isokinetic International, Chattanooga, TN, USA) with verbal encouragement. An investigator will choose the best of three sweeps (i.e., highest peak force) for analysis. The rate of force development and impulse will also be analyzed [51, 64].

Thigh isokinetic peak torque

Using the same settings as those used for the isometric peak strength exercise, the same tester will measure the dominant thigh isokinetic (at 60 degrees/second) force. With verbal encouragement, participants will perform maximal sweeps until peak values decline markedly from the best sweep on that particular test day, and the test is finished. An investigator will choose the best sweep (i.e., highest peak torque) and analyze the peak torque, total work, and angle at peak torque [51, 64].

Power rig

After recording the isometric and isokinetic peak torques, the tester will measure the maximum single-leg extensor power of the dominant leg using a University of Nottingham leg extensor power rig according to procedures described elsewhere [65]. This test is known to correlate with physical performance [8] even more than muscle strength [66] and serves to link the changes in strength to functional outcomes. During the test, participants are in a seated position, and a single maximum explosive leg extension accelerates a flywheel from rest. A computer calculates the power of the leg extensors on the basis of the speed of the flywheel. The participants familiarize themselves with the procedure by performing two warm-up trials followed by a minimum of five and a maximum of ten maximal trials with approximately 30 seconds of rest between them.

30-second chair-stand

The 30-second chair-stand test is a functional assessment of strength and endurance in the lower extremity by measuring the number of stands completed in 30 seconds with hands crossed on the chest [67]. The test is useful in characterizing the participant’s functionality and is also known to correlate with daily activity level [68].

Muscle biopsies and fiber-type composition

Working in sterile conditions, a qualified researcher will obtain muscle biopsies from the dominant m. vastus lateralis ad modum Bergström [69] with suction using the local anesthetic lidocaine 1 %. One biopsy is taken before and one after 12 months of the intervention, approximately 2–3 cm apart in a proximal-to-distal direction.

Body composition

A trained operator will perform whole-body DXA using the enCORE v.16 software (Lunar iDXA; GE Medical Systems, Pewaukee, WI, USA). Participants arrive having refrained from solid foods from 21:00 the day before the baseline and 12-month scans, and we perform scanning between 08:00 and 10:00. We obtain the remaining scans in the fed state at all times of the day, but prior to all scans participants are euhydrated and instructed to void. Whole-body composition is autoanalyzed, and, on a separate occasion, a blinded investigator performs separate thigh analyses manually with a region of interest (ROI) defined proximally by drawing a horizontal line laterally from the distal part of the groin and distally by drawing a line horizontally through the medial tibial plateau [7072]. The thigh ROIs are equal in size in each subject but vary with thigh length between subjects.

Bone mineral density

In the same baseline and 12-month DXA sessions, the same operator scans the bone mineral density in the lumbar region (L2–L4) and the dominant collum femoris. The investigator will place the vertebral ROI as caudal as possible, including the discus caudal to the vertebra, making sure not to include the adjacent vertebra or spinous process. On the collum femoris scan, the investigator places the ROI as distal as possible without including the greater trochanter [73, 74].

Gut microbiome

At 0, 6, and 12 months, participants place a fecal sample in an insulated bag with freezer elements until delivery at Bispebjerg Hospital within 48 h. The container is then stored at −60 °C until further analysis. Following homogenization, we extract the total DNA using the PowerSoil DNA isolation kit (MO BIO Laboratories, Carlsbad, CA, USA) with an initial bead-beating step and employ tag-encoded 16S ribosomal RNA gene (prokaryotes) and internal transcribed spacer region (eukaryotes) high-throughput sequencing using the MiSeq platform (Illumina, San Diego, CA, USA) to characterize the prokaryotic and eukaryotic components of the gut microbiome. As previously reported [75], we purify, extract, and sequence the virus-like particles for characterization of the gut virome.

Fecal metabolome

We will analyze the fecal metabolite extracts by performing gas chromatography with time-of-flight mass spectrometry (GC-TOF-MS) and nuclear magnetic resonance (NMR) spectroscopy using the same metabolite extract. The extraction procedure will involve stabilization of the homogenized fecal samples in PBS (pH 7.4) followed by freeze-drying and subsequent resuspension in methanol. We will perform untargeted metabolomics analysis and targeted profiling of short-chain fatty acids with a high-throughput GC-TOF-MS setup consisting of an Agilent 7890B gas chromatograph (Agilent Technologies, Santa Clara, CA, USA) and a high-throughput Pegasus TOF-MS spectrophotometer (LECO, St. Joseph, MI, USA). To increase GC-TOF-MS sensitivity toward a broad spectrum of nonvolatile metabolites, we will derivatize the samples as previously described [76] followed by processing of the obtained complex raw data using state-of-the-art three-way decomposition methods with Parallel Factor Analysis 2 [77]. We will further stabilize the fecal metabolite extracts using PBS prior to performing one-dimensional 1H NMR measurements using an AVANCE III 600 spectrometer (Bruker BioSpin GmbH, Rheinstetten, Germany) operating at a Larmor frequency of 600.13 MHz for protons and equipped with a cooled high-capacity autosampler (SampleJet; Bruker BioSpin GmbH) [78].

Plasma metabolome

We will collect the plasma samples with participants in fasting condition prior to the oral glucose tolerance test (OGTT) at 0 and 12 months and in fed condition at 6 and 18 months. Researchers will draw the blood into two containers, one containing tripotassium ethylenediaminetetraacetic acid (K3-EDTA) and one with heparin, and, after centrifugation at 3970 rpm for 10 minutes at 4 °C, we will pipette plasma into vials that are stored at −60 °C. We then will perform untargeted metabolomics of all samples using proton one-dimensional 1H NMR spectroscopy and for a subset of the samples by GC-TOF-MS.

We will perform the NMR measurements according to standard operating procedures developed for NMR metabolomics of plasma samples [79], and the data will provide unbiased metabolic fingerprints of the plasma samples, including information about lipoprotein particle distribution [80]. Prior to GC-TOF-MS analysis, we will thaw, vortex, and centrifuge the plasma samples at room temperature followed by addition of ice-cold acetonitrile in 1:3 (vol:vol) to precipitate proteins. Immediately after this, we will vortex the samples vigorously and centrifuge them at 20,000 × g for 10 minutes at 4 °C, and we will then dry 50 μl of clear supernatant under reduced pressure, derivatize it as described previously [76], and subject it to GC-TOF-MS.

Muscle protein synthesis

To assess MPS, we will perform an 8.5-h primed, continuous infusion of a stable isotope-labeled amino acid tracer, L-[ring-13C6]phenylalanine (Cambridge Isotope Laboratories, Tewksbury, MA, USA) at baseline and after 12 months of the intervention in a random subset of participants from each of the five intervention groups. The participants will fast overnight fast, and we will start the tracer infusion followed by collection of muscle biopsies after 1.5-h and 4.5-h infusions to measure the basal resting MPS over a 3-h period. Thereafter, we provide a protein drink as a beverage containing 20 g of whey hydrolysate plus 10 g of maltodextrin followed by collection of the third muscle biopsy. After an additional 4 h, we will measure the postprandial response of MPS. We will sample the muscle biopsies from the m. vastus lateralis muscle approximately 3 cm apart in a proximal-to-distal direction. Throughout the day, we will collect venous blood samples to measure tracer enrichment as well as amino acid and insulin concentrations, and we will measure the enrichment of phenylalanine tracer in blood and free in muscle cells by GC-MS/MS, whereas we will measure the incorporation of labeled phenylalanine in muscle proteins using GC-combustion/isotope ratio MS. We will then calculate the fractional synthesis rate (FSR) as the difference of incorporated tracer between subsequent biopsies (Ep2 − Ep1) divided by the precursor enrichment (Eprecursor; venous plasma and muscle free enrichment) and divided by incorporation time: FSR (%/h) = [(Ep2 − Ep1) × (Eprecursor)−1 × (incorporation time)−1] × 100 % [81]. This setup allows the evaluation of both basal MPS and the MPS response to a protein/carbohydrate drink before and after the 12-month intervention as a measure of muscle anabolic responsiveness to intake of a single protein supplement. We will also analyze muscle biopsies for relevant myocellular gene expression using real-time reverse transcriptase-polymerase chain reaction and for protein signaling through, for example, mammalian/mechanistic target of rapamycin complex 1 by performing Western blotting.

Tertiary outcomes

Blood parameters and anthropometry

At baseline, we will obtain standard health screening blood samples. At the remaining time points, we will measure only plasma HbA1c, cholesterol, and creatinine. Moreover, we will measure body weight, waist, and hip circumference at the remaining time points, whereas we will measure only height at baseline. Participants will wear underwear only when we measure body weight at baseline and 12 months, and they will wear light clothing at 6 and 18 months.

Hand grip strength

We will evaluate hand grip strength of the dominant hand (bilateral testing at baseline) by using a hand grip strength dynamometer (DHD-1 [SH1001]; SAEHAN Corporation, Changwon City, South Korea). The tester allows at least 30 seconds of rest between a minimum of three attempts, and the highest value at the given time point is used. The test is finished when two consecutive measurements are lower than the peak value [82].

400-m walk test (fast gait speed)

On a 20-m course marked with two colored cones, the physical examiner instructs the subject to walk 400 m as fast as possible without personal support or sitting down. Up to 1 minute of standing is permitted if the participant feels tired or experiences discomfort, as long as the test is completed within 15 minutes [83]. Among several functional measures, we chose 400-m gait speed as it is one of the more demanding functional tests available for elderly populations. This minimizes the risk of a ceiling effect among the included participants whom we expected to be relatively well-functioning.

Health-related questionnaires

At baseline and 12 months, we will hand out Danish translations of the 36-item Short Form Health Survey [84] and the Pittsburgh Sleep Quality Index [85], which the subjects complete without further instructions.

Activity monitoring

We measure 4-day (96-h) overall activity by mounting an activity monitor (activPal 3™, activPal 3c™, or activPal micro; PAL Technologies, Glasgow, UK) on the anterior surface of the thigh. The period always covers an entire weekend. An investigator will analyze data quantified as step counts, time in sitting/lying position, time in standing position, and time walking [86, 87].

Weighed food registration

We will administer a 3-day weighed food and liquid registration from Wednesday to Friday before, 4–6 weeks after, and 50 weeks after commencing the intervention. Investigators subsequently will quantify total daily intake and meal distribution of macronutrients using the MADLOG VITA system (MADLOG ApS, Kolding, Denmark).

Sensory and hedonic evaluation questionnaires

Participants will complete a questionnaire about their experience with taking the dietary supplements. We will collect baseline data on the first consumption day (day 0) and weekly for 12 weeks. Thereafter, we will reevaluate participants every third month until week 52. The flavor of the supplements will be alternated every week between fruit and cacao, and the participants will fill in the questionnaires within 1 minute after ingestion of the supplement. We will measure the participants’ acceptance after the first sip and after complete consumption on a 9-point hedonic scale ranging from 1 = “do not like at all” to 9 = “like a lot,” with neutral in the middle. After the first sip, we will record the participants’ perceived experiences using the check-all-that-apply method with 20 attributes of taste, flavor, mouth feel, and sensory appeal of the dietary supplement. After participants consume the supplements, we will collect data on how satiating, refreshing, and easy to drink it was, as well as on the strength and persistence of the aftertaste on a 9-point scale ranging from 1 = “completely disagree” to 9 = “completely agree,” with neutral in the middle.

Satisfaction with Food-related Life scale

Subjects will complete the Satisfaction with Food-related Life scale [88] on days 0, 90, 180, and 360 to identify factors contributing to satisfaction with food-related lifestyle.

Oral glucose tolerance test

Participants will undergo two OGTTs during the study. Participants will fast overnight, and a researcher will draw a basal venous blood sample and then administer 75 g of anhydrous glucose dissolved in 250 ml of tap water. Subsequently, the researcher will draw blood at 45 and 120 minutes after consumption of the glucose. Participants will lie supine throughout the 2-h period [89]. We will draw blood in K3-EDTA vials and cool the vials for at least 15 minutes, centrifuge the sample for 10 minutes at 3970 rpm at 4 °C, immediately analyze the plasma for glucose, and store aliquots at −80 °C for subsequent insulin measurement. At baseline, we draw two further blood samples in K3-EDTA and heparin vials respectively, which are cooled on ice, centrifuged for 10 minutes at 3172 × g, and the plasma is then stored at −60 °C until metabolome analysis.

Qualitative interviews and observations

Qualitative interviews

Upon participant inclusion, a researcher will conduct a short, standardized, qualitative interview with the participant to gain information on marital status, living conditions, work life, hobbies, and dietary preferences. At the 18-month follow-up, a researcher will interview the participants to gain information about their experience with the intervention and current habits regarding nutrition and exercise.

Questionnaire on food habits

At baseline, the staff will hand out a questionnaire with a range of questions about the food perceptions and habits of the participant. The questionnaire combines basic socioeducational data, quantitative questions, and quantifiable qualitative questions about lifestyle changes, dietary changes and perceptions, and intake of protein-rich foods. The participants can complete the questionnaires during the first 3 months and subsequently hand them in.

Life story interviews

We will select 25 of the intervention participants for 1.5- to 2.5-h life story/trajectory interviews in their own homes. The main themes of the interviews are past and present perceptions of and habits concerning food and physical activity, but we will also include questions regarding mobility and work life.

Ethnographic fieldwork

We will select 48 research participants to engage in a qualitative study focused on everyday life routines, eating practices, physical activity, and experiences with participating in the trial. We do this by observing the research participants in their homes and at the site of the clinical trial, thereby following their daily activities in both settings. Further, we will conduct semistructured qualitative interviews to gain in-depth knowledge about selected individuals.

Data collection, management, and analysis

According to Danish research ethics legislation, we will inform the participants about their rights as voluntary subjects in a scientific trial and interview them about their motivation for participation. We do this to make participants consider participation thoroughly to diminish the likelihood of their dropping out. We are obliged to report any unforeseen adverse effects due to participation to the local ethical committee, which will consider terminating the project depending on the reported incidents.

We will collect and manage study data using REDCap electronic data capture tools hosted at RegionH (Copenhagen, Denmark) [90] and secure it according to Danish Data Protection Agency legislation (approval on 9 January 2015, number 2012-58-0004 – BBH-2015-001 I-Suite 03432). This includes pseudoanonymization of sensitive data. Only relevant staff have logged access to the key file.

The project has a scientific advisory board with experts from all scientific disciplines applied in the research, and its members meet annually with the researchers to discuss progress and plan future work. The board members are financially and personally unrelated to the study investigators and the project as such.

The principal investigators from each scientific discipline have a shared responsibility to secure and monitor data collection and interpretation, and thus they are all involved in project management, analysis of samples, data collection, and observations and will all have access to the final dataset and jointly be involved in the interpretation of results.

Statistics and power calculations

For all power calculations, we will apply a level of significance of 0.05 and a power of 0.80, and the primary outcome is the degree of muscle hypertrophy determined as the change in quadriceps muscle CSA on MRI scans over the course of the 12-month intervention. On the basis of previous data [91], we set the following parameters: least average expected change over the course of the 12-month intervention between distinct intervention groups, 300 mm2 (corresponding to an estimated relative change of 6.5 %) with a SD set to 140 mm2. Our goal is to be able to detect differences between groups’ mean hypertrophy down to approximately 2 %, which corresponds to approximately 80 mm2. A difference in the change of quadriceps muscle CSA greater than 80 mm2 between any two groups is statistically detectable with a power of at least 0.80 with a group size of 30. We decided to include 35 subjects in each of the three groups (HRTW, LITW, and CARB), as we anticipated a dropout rate of 15 % at the time of study initiation. In the WHEY and COLL groups, we expected a larger dropout rate because of taste issues with protein supplements and no motivational effect by training as in the HRTW and LITW groups. Thus, we will include 50 each in the WHEY and COLL groups. On an ad hoc basis, we will evaluate the actual dropout rate and adjust the total number of inclusions accordingly.

In a subset of participants from the five intervention groups, we will conduct experimental tracer studies before and after the 12-month intervention period to determine the extent to which the interventions affect the responsiveness of MPS to protein intake. The goal is to measure whether any of the interventions enhance the anabolic sensitivity to protein intake and thereby investigate if we can link the observed differences in muscle mass over the course of the intervention period to changes in the anabolic response to protein feeding. Thus, the outcome measure is the relative change in the FSR of muscle proteins from the basal condition, defined as the overnight fasted and resting state, to the average 4-h postprandial period after intake of a standard protein bolus. We are not aware of any data on changes in MPS responsiveness over time, and we therefore based the power calculations on a sum of our own published and unpublished data obtained in healthy elderly participants. We established the following parameters: expected average 4-h postprandial responsiveness to protein feeding from basal condition of 0.016 %/h (i.e., approximately corresponding to an increase in muscle protein FSR of 50 %) with an interparticipant SD on the increase of 0.017 %/h. We aim to be able to detect a 50 % change in responsiveness within a group over the course of the 12-month period (i.e., a change of 0.008 %/h in the increase in the muscle protein FSR from 0.016 %/h to 0.024 %/h). The SD for the change in responsiveness is set as 0.006 %/h. By including 10–12 participants, we obtain a power of at least 0.80 to detect a statistical difference over time within each intervention group. By taking the probability of dropouts into account, we decided to include 12 participants in the HRTW, LITW, and CARB groups and 15 in the WHEY and COLL groups, respectively.

Investigators will analyze data as intention-to-treat (except in subgroup analyses) as well as per protocol, and we will test the following groups against each other using one-way analysis of variance of the relative group changes from baseline to 12 months: (1) WHEY vs. COLL vs. CARB and (2) HRTW vs. LITW vs. WHEY. We will not conduct any interim analyses in this project.

Discussion

The inclusion rate is currently 15 %, and we have been in contact with more than 1000 elderly individuals, recruiting approximately 150 participants. The major challenge until now has been to find elderly individuals who are willing to be committed to participate for 18 months as well as accept the outcome of the randomization. The interventions are very different in terms of time required for the intervention as well as potential physical and social gain. The three supplementation-only groups demand less time, but the candidates expect less gain from the intervention than those in the more time-consuming physical training groups, so the main reasons for candidates to decline participation is the uncertainty of the randomization. This has resulted in roughly 45 % of candidates having decided to refrain from volunteering. Second, our exclusion criteria are numerous in order to ensure accurate results, and we have excluded around 40 % of the potential participant population for this reason, leaving for inclusion 15 % of those who had initially contacted us. The invasive procedures and number of tests are not issues of major importance for the participants when we are recruiting. Fortunately, the dropout rate until now has been very low: Only 4.3 % have ceased participation, which is far less than the initially expected rate of 10–15 %. We recruit research participants for this study in the Capital Region of Denmark. The population in this region is wealthier [92], and likely also healthier, than the general population in Denmark, but for practical reasons we could only recruit participants locally. This represents a possible selection bias in the trial.

Safety issues and dropout rate

We consider the project safe, and the clinical staff routinely perform the tests and invasive procedures used. We supervise participants during heavy strength training and carefully instruct them in the light load training exercises. The nutrient supplements contain 510 kJ (i.e., a dose of 1020 kJ/day) and induce mild satiety when ingested. Therefore, we expect a slight decrease in the participants’ habitual diet corresponding to the energy content in the supplements, and we expect their body weight to be stable. We control HbA1c, creatinine, and cholesterol levels after 6 months to ensure that participants do not develop insulin resistance, impaired kidney function, or hyperlipidemia because of supplement intake. In case of adverse events, the Danish Patient Compensation Association covers participants’ treatment needs.

Perspectives

The purpose of the CALM Intervention Study is to provide interdisciplinary knowledge on the efficacy and feasibility of daily protein supplementation and muscular exercise training in different settings to support the maintenance of skeletal muscle mass in the elderly to ensure daily functional capacity and maintain or improve quality of life. Until now, only a few studies have investigated the physiological outcome of strength training and protein supplementation, and, to our knowledge, a light intensity strength training regime has never been investigated. Further, the interdisciplinary setting, including sensory, fecal, and qualitative investigations, will provide unique insights into how to counteract the age-related loss of skeletal muscle mass.

A key assumption in the CALM Intervention Study is that an improved adherence to a healthier lifestyle requires sensitivity to the everyday lives, routines, and cultural differences of the research participants [93]. We aim to enhance this sensitivity by gathering qualitative knowledge on the societal, cultural, and individual factors that influence daily routines related to nutrient consumption and exercise to identify and circumvent the barriers that complicate changes in these. We will perform an exploration of societal and individual influences on everyday lifestyle habits and of how the project configures the participants’ everyday food and exercise practices. Further, we will investigate how participants’ practices are (and are not) transformed by their interactions with the materiality as well as the scientific practitioners and attitudes of the project to provide insights that potentially could improve health promotion strategies [94].

We expect to translate the results of this into guidelines targeted to the home-dwelling, independent elderly population and disseminate them to the public through channels involving nongovernmental organizations, newspapers, electronic media, and stakeholders in, for example, the municipalities. The CALM Intervention Study will provide novel information on the potential longitudinal benefits of protein supplementation and strength training regarding physical performance, gut microbiota, and overall health while taking into account the feasibility and impact of the interventions on everyday life.

Trial status

Since we began advertising for participants in January 2014, we have recruited and randomized 153 (as of 1 August 2016). A total of 54 and 32 participants have completed the intervention and follow-up, respectively.

Abbreviations

CALM, Counteracting Age-related Loss of Skeletal Muscle Mass; CARB, carbohydrate supplementation group; COLL, collagen supplementation group; COPD, chronic obstructive pulmonary disease; CSA, cross-sectional area; DXA, dual-energy X-ray absorptiometry; EULAR, European League Against Rheumatism; FEV1, forced expiratory volume in 1 second; FSR, fractional synthesis rate; FVC, forced vital capacity; GC-TOF-MS, gas chromatography with time-of-flight mass spectrometry; GOLD, Global Initiative for Chronic Obstructive Lung Disease; HRTW, heavy resistance training with whey supplementation; ITS, internal transcribed spacer; K3-EDTA, tripotassium ethylenediaminetetraacetic acid; LITW, light-intensity training with whey supplementation; LVEF, left ventricular ejection fraction; MPS, muscle protein synthesis; MRI, magnetic resonance imaging; NMR, nuclear magnetic resonance; OGTT, oral glucose tolerance test; PDCAAS, protein digestibility-corrected amino acid score; PSQI, Pittsburgh Sleep Quality Index; RCT, randomized controlled trial; RM, repetition maximum; ROI, region of interest; SF-36, 36-item Short Form Health Survey; SPIRIT, Standard Protocol Items: Recommendations for Interventional Trials; SWFL, Satisfaction with Food-related Life scale; WHEY, whey supplementation

References

  1. National Institute on Aging, National Institutes of Health (NIH), World Health Organization. Global health and aging. NIH Publication 11-7737. Washington, DC: NIH; October 2011. http://www.who.int/ageing/publications/global_health.pdf. Accessed 27 Jul 2016.

  2. Population Division, Department of Economic Affairs, United Nations. World population prospects: the 2015 revision. Geneva, Switzerland: United Nations; 2015. https://esa.un.org/unpd/wpp/. Accessed 27 Jul 2016.

    Google Scholar 

  3. Fried LP, Tangen CM, Walston J, Newman AB, Hirsch C, Gottdiener J, et al. Frailty in older adults: evidence for a phenotype. J Gerontol A Biol Sci Med Sci. 2001;56:M146–56.

    Article  CAS  PubMed  Google Scholar 

  4. Rosenberg IH. Summary comments. Am J Clin Nutr. 1989;50:1231–3.

    Google Scholar 

  5. Janssen I, Heymsfield SB, Wang ZM, Ross R. Skeletal muscle mass and distribution in 468 men and women aged 18-88 yr. J Appl Physiol. 2000;89:81–8.

    CAS  PubMed  Google Scholar 

  6. Frontera WR, Hughes VA, Fielding RA, Fiatarone MA, Evans WJ, Roubenoff R. Aging of skeletal muscle: a 12-yr longitudinal study. J Appl Physiol. 2000;88:1321–6.

    CAS  PubMed  Google Scholar 

  7. Gallagher D, Ruts E, Visser M, Heshka S, Baumgartner RN, Wang J, et al. Weight stability masks sarcopenia in elderly men and women. Am J Physiol Endocrinol Metab. 2000;279:E366–75.

    CAS  PubMed  Google Scholar 

  8. Skelton DA, Greig CA, Davies JM, Young A. Strength, power and related functional ability of healthy people aged 65-89 years. Age Ageing. 1994;23:371–7.

    Article  CAS  PubMed  Google Scholar 

  9. Landi F, Liperoti R, Russo A, Giovannini S, Tosato M, Capoluongo E, et al. Sarcopenia as a risk factor for falls in elderly individuals: results from the ilSIRENTE study. Clin Nutr. 2012;31:652–8.

    Article  PubMed  Google Scholar 

  10. Friedman EM, Ryff CD. Living well with medical comorbidities: a biopsychosocial perspective. J Gerontol B Psychol Sci Soc Sci. 2012;67:535–44.

    Article  PubMed  PubMed Central  Google Scholar 

  11. da Silva AT, de Oliveira Duarte YA, Ferreira Santos JL, Wong R, Lebrão ML. Sarcopenia according to the European Working Group on Sarcopenia in Older People (EWGSOP) versus dynapenia as a risk factor for mortality in the elderly. J Nutr Health Aging. 2014;18:751–6.

    Article  Google Scholar 

  12. Morley JE, Abbatecola AM, Argiles JM, Baracos V, Bauer J, Bhasin S, et al. Sarcopenia with limited mobility: an international consensus. J Am Med Dir Assoc. 2011;12:403–9.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Deutz NEP, Bauer JM, Barazzoni R, Biolo G, Boirie Y, Bosy-Westphal A, et al. Protein intake and exercise for optimal muscle function with aging: recommendations from the ESPEN Expert Group. Clin Nutr. 2014;33:929–36.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Cruz-Jentoft AJ. Perspective: Protein and exercise for frailty and sarcopenia: still learning. J Am Med Dir Assoc. 2013;14:69–71.

    Article  PubMed  Google Scholar 

  15. Moore DR, Churchward-Venne TA, Witard O, Breen L, Burd NA, Tipton KD, et al. Protein ingestion to stimulate myofibrillar protein synthesis requires greater relative protein intakes in healthy older versus younger men. J Gerontol A Biol Sci Med Sci. 2015;70:57–62.

    Article  PubMed  Google Scholar 

  16. Kumar V, Selby A, Rankin D, Patel R, Atherton P, Hildebrandt W, et al. Age-related differences in the dose-response relationship of muscle protein synthesis to resistance exercise in young and old men. J Physiol. 2009;587(Pt 1):211–7.

    Article  CAS  PubMed  Google Scholar 

  17. Kumar V, Atherton PJ, Selby A, Rankin D, Williams J, Smith K, et al. Muscle protein synthetic responses to exercise: effects of age, volume, and intensity. J Gerontol A Biol Sci Med Sci. 2012;67:1170–7.

    Article  PubMed  Google Scholar 

  18. Tieland M, Dirks ML, van der Zwaluw N, Verdijk LB, van de Rest O, de Groot LCPGM, et al. Protein supplementation increases muscle mass gain during prolonged resistance-type exercise training in frail elderly people: a randomized, double-blind, placebo-controlled trial. J Am Med Dir Assoc. 2012;13:713–9.

    Article  PubMed  Google Scholar 

  19. Fiatarone MA, O’Neill EF, Ryan ND, Clements KM, Solares GR, Nelson ME, et al. Exercise training and nutritional supplementation for physical frailty in very elderly people. N Engl J Med. 1994;330:1769–75.

    Article  CAS  PubMed  Google Scholar 

  20. Bartali B, Frongillo EA, Bandinelli S, Lauretani F, Semba RD, Fried LP, et al. Low nutrient intake is an essential component of frailty in older persons. J Gerontol A Biol Sci Med Sci. 2006;61:589–93.

    Article  PubMed  PubMed Central  Google Scholar 

  21. Beasley JM, Shikany JM, Thomson CA. The role of dietary protein intake in the prevention of sarcopenia of aging. Nutr Clin Pract. 2013;28:684–90.

    Article  PubMed  PubMed Central  Google Scholar 

  22. Beasley JM, Wertheim BC, LaCroix AZ, Prentice RL, Neuhouser ML, Tinker LF, et al. Biomarker-calibrated protein intake and physical function in the Women’s Health Initiative. J Am Geriatr Soc. 2013;61:1863–71.

    Article  PubMed  PubMed Central  Google Scholar 

  23. Tieland M, van de Rest O, Dirks ML, van der Zwaluw N, Mensink M, van Loon LJC, et al. Protein supplementation improves physical performance in frail elderly people: a randomized, double-blind, placebo-controlled trial. J Am Med Dir Assoc. 2012;13:720–6.

    Article  PubMed  Google Scholar 

  24. Zhu K, Kerr DA, Meng X, Devine A, Solah V, Binns CW, et al. Two-year whey protein supplementation did not enhance muscle mass and physical function in well-nourished healthy older postmenopausal women. J Nutr. 2015;145:2520–6.

    Article  CAS  PubMed  Google Scholar 

  25. Bauer JM, Verlaan S, Bautmans I, Brandt K, Donini LM, Maggio M, et al. Effects of a vitamin D and leucine-enriched whey protein nutritional supplement on measures of sarcopenia in older adults, the PROVIDE Study: a randomized, double-blind, placebo-controlled trial. J Am Med Dir Assoc. 2015;16:740–7.

    Article  PubMed  Google Scholar 

  26. Cuthbertson D, Smith K, Babraj J, Leese G, Waddell T, Atherton P, et al. Anabolic signaling deficits underlie amino acid resistance of wasting, aging muscle. FASEB J. 2005;19:422–4.

    CAS  PubMed  Google Scholar 

  27. Pennings B, Groen B, de Lange A, Gijsen AP, Zorenc AH, Senden JMG, et al. Amino acid absorption and subsequent muscle protein accretion following graded intakes of whey protein in elderly men. Am J Physiol Endocrinol Metab. 2012;302:E992–9.

    Article  CAS  PubMed  Google Scholar 

  28. Yang Y, Breen L, Burd NA, Hector AJ, Churchward-Venne TA, Josse AR, et al. Resistance exercise enhances myofibrillar protein synthesis with graded intakes of whey protein in older men. Br J Nutr. 2012;108:1780–8.

    Article  CAS  PubMed  Google Scholar 

  29. World Health Organization (WHO), Food and Agriculture Organization of the United Nations (FAO), United Nations University (UNU). Protein and amino acid requirements in human nutrition: report of a joint FAO/WHO/UNU expert consultation. WHO technical report series 935. Geneva, Switzerland: WHO; 2007. http://www.who.int/nutrition/publications/nutrientrequirements/WHO_TRS_935/en/. Accessed 27 Jul 2016.

    Google Scholar 

  30. Devries MC, Breen L, Von Allmen M, MacDonald MJ, Moore DR, Offord EA, et al. Low-load resistance training during step-reduction attenuates declines in muscle mass and strength and enhances anabolic sensitivity in older men. Physiol Rep. 2015;3(8):e12493.

    Article  PubMed  PubMed Central  Google Scholar 

  31. Pennings B, Boirie Y, Senden JMG, Gijsen AP, Kuipers H, Van Loon LJC. Whey protein stimulates postprandial muscle protein accretion more effectively than do casein and casein hydrolysate in older men. Am J Clin Nutr. 2011;93:997–1005.

    Article  CAS  PubMed  Google Scholar 

  32. Volpi E, Campbell WW, Dwyer JT, Johnson MA, Jensen GL, Morley JE, et al. Is the optimal level of protein intake for older adults greater than the recommended dietary allowance? J Gerontol A Biol Sci Med Sci. 2013;68:677–81.

    Article  CAS  PubMed  Google Scholar 

  33. Christensen AI, Davidsen M, Ekholm O, Pedersen PV, Juel K. Danskernes sundhed – den nationale sundhedsprofil 2013 [in Danish]. Copenhagen: Sundhedsstyrelsen; 2014. https://sundhedsstyrelsen.dk/da/sundhed-og-livsstil/~/media/1529A4BCF9C64905BAC650B6C45B72A5.ashx.

    Google Scholar 

  34. Pedersen AN, Christensen T, Matthiessen J, Knudsen VK, Rosenlund-Sørensen M, Biltoft-Jensen A, et al. Danskernes kostvaner 2011-2013 [in Danish]. Søborg, Denmark: DTU Fødevareinstituttet; 2015.

    Google Scholar 

  35. Pedersen AN, Cederholm T. Health effects of protein intake in healthy elderly populations: a systematic literature review. Food Nutr Res. 2014;58:23364.

    Article  Google Scholar 

  36. Bauer J, Biolo G, Cederholm T, Cesari M, Cruz-Jentoft AJ, Morley JE, et al. Evidence-based recommendations for optimal dietary protein intake in older people: a position paper from the PROT-AGE Study Group. J Am Med Dir Assoc. 2013;14:542–59.

    Article  PubMed  Google Scholar 

  37. Paddon-Jones D, Short KR, Campbell WW, Volpi E, Wolfe RR. Role of dietary protein in the sarcopenia of aging. Am J Clin Nutr. 2008;87:1562S–6S.

    CAS  PubMed  Google Scholar 

  38. European Food Safety Authority (EFSA) Panel on Dietetic Products, Nutrition and Allergies. Scientific opinion on dietary reference values for protein. EFSA J. 2012;10(2):2557. doi:10.2903/j.efsa.2012.2557.

    Article  Google Scholar 

  39. Berner LA, Becker G, Wise M, Doi J. Characterization of dietary protein among older adults in the United States: amount, animal sources, and meal patterns. J Acad Nutr Diet. 2013;113:809–15.

    Article  PubMed  Google Scholar 

  40. Areta JL, Burke LM, Ross ML, Camera DM, West DWD, Broad EM, et al. Timing and distribution of protein ingestion during prolonged recovery from resistance exercise alters myofibrillar protein synthesis. J Physiol. 2013;591(Pt 9):2319–31.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Paddon-Jones D, Campbell WW, Jacques PF, Kritchevsky SB, Moore LL, Rodriguez NR, et al. Protein and healthy aging. Am J Clin Nutr. 2015;101:1339S–45S.

  42. Bollwein J, Diekmann R, Kaiser MJ, Bauer JM, Uter W, Sieber CC, et al. Distribution but not amount of protein intake is associated with frailty: a cross-sectional investigation in the region of Nürnberg. Nutr J. 2013;12:109.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Mamerow MM, Mettler JA, English KL, Casperson SL, Arentson-Lantz E, Sheffield-Moore M, et al. Dietary protein distribution positively influences 24-h muscle protein synthesis in healthy adults. J Nutr. 2014;144:876–80.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  44. Pérez-Cueto FJA, Aschemann-Witzel J, Shankar B, Brambila-Macias J, Bech-Larsen T, Mazzocchi M, et al. Assessment of evaluations made to healthy eating policies in Europe: a review within the EATWELL Project. Public Health Nutr. 2012;15:1489–96.

    Article  PubMed  Google Scholar 

  45. Abe T, Mitsukawa N, Thiebaud RS, Loenneke JP, Loftin M, Ogawa M. Lower body site-specific sarcopenia and accelerometer-determined moderate and vigorous physical activity: the HIREGASAKI study. Aging Clin Exp Res. 2012;24:657–62.

    PubMed  Google Scholar 

  46. American College of Sports Medicine. Progression models in resistance training for healthy adults. Med Sci Sports Exerc. 2009;41:687–708.

    Article  Google Scholar 

  47. Campos GER, Luecke TJ, Wendeln HK, Toma K, Hagerman FC, Murray TF, et al. Muscular adaptations in response to three different resistance-training regimens: specificity of repetition maximum training zones. Eur J Appl Physiol. 2002;88:50–60.

    Article  PubMed  Google Scholar 

  48. Holm L, Reitelseder S, Pedersen TG, Doessing S, Petersen SG, Flyvbjerg A, et al. Changes in muscle size and MHC composition in response to resistance exercise with heavy and light loading intensity. J Appl Physiol. 2008;105:1454–61.

    Article  CAS  PubMed  Google Scholar 

  49. Arnarson A, Gudny Geirsdottir O, Ramel A, Briem K, Jonsson PV, Thorsdottir I. Effects of whey proteins and carbohydrates on the efficacy of resistance training in elderly people: double blind, randomised controlled trial. Eur J Clin Nutr. 2013;67:821–6.

    Article  CAS  PubMed  Google Scholar 

  50. Sipilä S, Suominen H. Effects of strength and endurance training on thigh and leg muscle mass and composition in elderly women. J Appl Physiol. 1995;78:334–40.

    PubMed  Google Scholar 

  51. Kryger AI, Andersen JL. Resistance training in the oldest old: consequences for muscle strength, fiber types, fiber size, and MHC isoforms. Scand J Med Sci Sports. 2007;17:422–30.

    Article  CAS  PubMed  Google Scholar 

  52. Stewart VH, Saunders DH, Greig CA. Responsiveness of muscle size and strength to physical training in very elderly people: a systematic review. Scand J Med Sci Sports. 2014;24:e1–10.

    Article  CAS  PubMed  Google Scholar 

  53. King AC. Interventions to promote physical activity by older adults. J Gerontol A Biol Sci Med Sci. 2001;56(Spec No 2):36–46.

    Article  PubMed  Google Scholar 

  54. King AC, Castro C, Wilcox S, Eyler AA, Sallis JF, Brownson RC. Personal and environmental factors associated with physical inactivity among different racial-ethnic groups of U.S. middle-aged and older-aged women. Health Psychol. 2000;19:354–64.

    Article  CAS  PubMed  Google Scholar 

  55. King AC, Haskell WL, Taylor CB, Kraemer HC, DeBusk RF. Group- vs home-based exercise training in healthy older men and women: a community-based clinical trial. JAMA. 1991;266:1535–42.

    Article  CAS  PubMed  Google Scholar 

  56. Perri MG, Martin AD, Leermakers EA, Sears SF, Notelovitz M. Effects of group- versus home-based exercise in the treatment of obesity. J Consult Clin Psychol. 1997;65:278–85.

    Article  CAS  PubMed  Google Scholar 

  57. Daley AJ, Maynard IW. Preferred exercise mode and affective responses in physically active adults. Psychol Sport Exerc. 2003;4:347–56.

    Article  Google Scholar 

  58. MinimPy 0.3. https://sourceforge.net/projects/minimpy/. Accessed 27 Jul 2016.

  59. Saghaei M, Saghaei S. Implementation of an open-source customizable minimization program for allocation of patients to parallel groups in clinical trials. J Biomed Sci Eng. 2011;4:734–9.

    Article  Google Scholar 

  60. Brzycki M. A practical approach to strength training. 1st ed. Grand Rapids, MI: Masters Press; 1989.

    Google Scholar 

  61. Mitsiopoulos N, Baumgartner RN, Heymsfield SB, Lyons W, Gallagher D, Ross R. Cadaver validation of skeletal muscle measurement by magnetic resonance imaging and computerized tomography. J Appl Physiol. 1998;85:115–22.

    CAS  PubMed  Google Scholar 

  62. Fuller NJ, Hardingham CR, Graves M, Screaton N, Dixon AK, Ward LC, et al. Assessment of limb muscle and adipose tissue by dual-energy X-ray absorptiometry using magnetic resonance imaging for comparison. Int J Obes. 1999;23:1295–302.

    Article  CAS  Google Scholar 

  63. Maden-Wilkinson TM, Degens H, Jones DA, McPhee JS. Comparison of MRI and DXA to measure muscle size and age-related atrophy in thigh muscles. J Musculoskelet Neuronal Interact. 2013;13:320–8.

    CAS  PubMed  Google Scholar 

  64. Farrell M, Richards JG. Analysis of the reliability and validity of the kinetic communicator exercise device. Med Sci Sports Exerc. 1986;18:44–9.

    Article  CAS  PubMed  Google Scholar 

  65. Bassey EJ, Short AH. A new method for measuring power output in a single leg extension: feasibility, reliability and validity. Eur J Appl Physiol. 1990;60:385–90.

    Article  CAS  Google Scholar 

  66. Reid KF, Fielding RA. Skeletal muscle power: a critical determinant of physical functioning in older adults. Exerc Sport Sci Rev. 2012;40:4–12.

    Article  PubMed  PubMed Central  Google Scholar 

  67. Jones CJ, Rikli RE, Beam WC. A 30-s chair-stand test as a measure of lower body strength in community-residing older adults. Res Q Exerc Sport. 1999;70:113–9.

    Article  CAS  PubMed  Google Scholar 

  68. Rikli RE, Jones CJ. Development and validation of criterion-referenced clinically relevant fitness standards for maintaining physical independence in later years. Gerontologist. 2013;53:255–67.

    Article  PubMed  Google Scholar 

  69. Bergström J. Percutaneous needle biopsy of skeletal muscle in physiological and clinical research. Scand J Clin Lab Invest. 1975;35:609–16.

    Article  PubMed  Google Scholar 

  70. Andreoli A, Scalzo G, Masala S, Tarantino U, Guglielmi G. Body composition assessment by dual-energy X-ray absorptiometry (DXA). Radiol Med. 2009;114:286–300.

    Article  CAS  PubMed  Google Scholar 

  71. Nana A, Slater GJ, Stewart AD, Burke LM. Methodology review: using dual-energy X-ray absorptiometry (DXA) for the assessment of body composition in athletes and active people. Int J Sport Nutr Exerc Metab. 2015;25:198–215.

    Article  PubMed  Google Scholar 

  72. Marinangeli CPF, Kassis AN. Use of dual X-ray absorptiometry to measure body mass during short- to medium-term trials of nutrition and exercise interventions. Nutr Rev. 2013;71:332–42.

    Article  PubMed  Google Scholar 

  73. Leslie WD, Adler RA, El-Hajj Fuleihan G, Hodsman AB, Kendler DL, McClung M, et al. Application of the 1994 WHO classification to populations other than postmenopausal Caucasian women: the 2005 ISCD Official Positions. J Clin Densitom. 1994;2006(9):22–30.

    Google Scholar 

  74. Kanis JA, Melton LJ, Christiansen C, Johnston CC, Khaltaev N. The diagnosis of osteoporosis. J Bone Miner Res. 1994;9:1137–41.

    Article  CAS  PubMed  Google Scholar 

  75. Castro-Mejía JL, Muhammed MK, Kot W, Neve H, Franz CMAP, Hansen LH, et al. Optimizing protocols for extraction of bacteriophages prior to metagenomic analyses of phage communities in the human gut. Microbiome. 2015;3:64.

    Article  PubMed  PubMed Central  Google Scholar 

  76. Khakimov B, Motawia MS, Bak S, Engelsen SB. The use of trimethylsilyl cyanide derivatization for robust and broad-spectrum high-throughput gas chromatography-mass spectrometry based metabolomics. Anal Bioanal Chem. 2013;405:9193–205.

    Article  CAS  PubMed  Google Scholar 

  77. Khakimov B, Amigo JM, Bak S, Engelsen SB. Plant metabolomics: resolution and quantification of elusive peaks in liquid chromatography-mass spectrometry profiles of complex plant extracts using multi-way decomposition methods. J Chromatogr A. 2012;1266:84–94.

    Article  CAS  PubMed  Google Scholar 

  78. Savorani F, Rasmussen MA, Mikkelsen MS, Engelsen SB. A primer to nutritional metabolomics by NMR spectroscopy and chemometrics. Food Res Int. 2013;54:1131–45.

    Article  CAS  Google Scholar 

  79. Savorani F, Kristensen M, Larsen FH, Astrup A, Engelsen SB. High throughput prediction of chylomicron triglycerides in human plasma by nuclear magnetic resonance and chemometrics. Nutr Metab (Lond). 2010;7:43.

    Article  Google Scholar 

  80. Petersen M, Dyrby M, Toubro S, Engelsen SB, Nørgaard L, Pedersen HT, et al. Quantification of lipoprotein subclasses by proton nuclear magnetic resonance-based partial least-squares regression models. Clin Chem. 2005;51:1457–61.

    Article  CAS  PubMed  Google Scholar 

  81. Wolfe RR, Chinkes DL. Isotope tracers in metabolic research: principles and practice of kinetic analysis. 2nd ed. Hoboken, NJ: Wiley-Liss; 2005.

    Google Scholar 

  82. Dodds RM, Syddall HE, Cooper R, Benzeval M, Deary IJ, Dennison EM, et al. Grip strength across the life course: normative data from twelve British studies. PLoS One. 2014;9:e113637.

    Article  PubMed  PubMed Central  Google Scholar 

  83. Simonsick EM, Fan E, Fleg JL. Estimating cardiorespiratory fitness in well-functioning older adults: treadmill validation of the long distance corridor walk. J Am Geriatr Soc. 2006;54:127–32.

    Article  PubMed  Google Scholar 

  84. Maruish ME. User’s manual for the SF-36v2 Health Survey. 3rd ed. Lincoln, RI: QualityMetric Incorporated; 2011.

    Google Scholar 

  85. Buysse DJ, Reynolds CF, Monk TH, Berman SR, Kupfer DJ. The Pittsburgh Sleep Quality Index: a new instrument for psychiatric practice and research. Psychiatry Res. 1989;28:193–213.

    Article  CAS  PubMed  Google Scholar 

  86. Dowd KP, Harrington DM, Donnelly AE. Criterion and concurrent validity of the activPALTM professional physical activity monitor in adolescent females. PLoS One. 2012;7:e47633.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  87. Dahlgren G, Carlsson D, Moorhead A, Häger-Ross C, McDonough SM. Test-retest reliability of step counts with the ActivPALTM device in common daily activities. Gait Posture. 2010;32:386–90.

    Article  PubMed  Google Scholar 

  88. Grunert KG, Dean M, Raats MM, Nielsen NA, Lumbers M. A measure of satisfaction with food-related life. Appetite. 2007;49:486–93.

    Article  PubMed  Google Scholar 

  89. World Health Organization (WHO). Definition, diagnosis, and classification of diabetes mellitus and its complications: report of a WHO consultation. Geneva, Switzerland: WHO; 1999.

    Google Scholar 

  90. Harris PA, Taylor R, Thielke R, Payne J, Gonzalez N, Conde JG. Research Electronic Data Capture (REDCap)—a metadata driven methodology and workflow process for providing translational research informatict support. J Biomed Inform. 2009;42:377–81.

    Article  PubMed  Google Scholar 

  91. Holm L, Olesen JL, Matsumoto K, Doi T, Mizuno M, Alsted TJ, et al. Protein-containing nutrient supplementation following strength training enhances the effect on muscle mass, strength, and bone formation in postmenopausal women. J Appl Physiol. 2008;105:274–81.

    Article  CAS  PubMed  Google Scholar 

  92. Danmarks Statistik. Danmark i tal 2015 [in Danish]. http://www.dst.dk/Site/Dst/Udgivelser/GetPubFile.aspx?id=19005&sid=dk2015. Accessed 27 Jul 2016.

  93. Mathar T, Jansen YJFM. Health promotion and prevention programmes in practice. how patient’s health practices are rationalised, reconceptualised and reorganised. Bielefeld, Germany: transcript Verlag; 2010.

    Book  Google Scholar 

  94. Jespersen AP, Bønnelycke J, Eriksen HH. Careful science? Bodywork and care practices in randomised clinical trials. Sociol Health Illn. 2014;36:655–69.

    Article  PubMed  Google Scholar 

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Acknowledgements

We thank the Danish National Health Survey and Sisse Fagt, National Food Institute, Technical University of Denmark for sharing data with us. Further, we thank PROcare ApS (Roskilde, Denmark) and Hygenic Corp. (Akron, OH, USA) for kindly providing the rubber bands used for training.

Funding

The study is funded by the University of Copenhagen Excellence Programme for Interdisciplinary Research 2016 (CALM project; www.calm.ku.dk). In addition, the following other funding sources have supported the project: Innovation Fund Denmark (project title: COUNTERSTRIKE), the Danish Dairy Research Foundation, the Municipality of Copenhagen, Arla Foods Ingredients P/S, the University of Copenhagen Faculty of Health and Medical Sciences, and stipends from the University of Copenhagen and the Malaysian government.

Authors’ contributions

RB conceived and designed the study, drafted the manuscript, and read and approved the final version. SR conceived and designed the study, drafted the manuscript, and read and approved the final version. GH conceived and designed the study, revised the manuscript critically, and read and approved the final version. JC conceived and designed the study, drafted the manuscript, and read and approved the final version. BK conceived and designed the study, revised the manuscript critically, and read and approved the final version. FA conceived and designed the study, revised the manuscript critically, and read and approved the final version. MK conceived and designed the study, revised the manuscript critically, and read and approved the final version. SE conceived and designed the study, revised the manuscript critically, and read and approved the final version. SJ conceived and designed the study, drafted the manuscript, and read and approved the final version. MR conceived and designed the study, revised the manuscript critically, and read and approved the final version. AL conceived and designed the study, revised the manuscript critically, and read and approved the final version. TJ conceived and designed the study, drafted the manuscript, and read and approved the final version. NB conceived and designed the study, revised the manuscript critically, and read and approved the final version. AS conceived and designed the study, revised the manuscript critically, and read and approved the final version. FP conceived and designed the study, revised the manuscript critically, and read and approved the final version. DN conceived and designed the study, revised the manuscript critically, and read and approved the final version. AJ conceived and designed the study, drafted the manuscript, and read and approved the final version. LH conceived of and designed the study, drafted the manuscript, and read and approved the final version.

Competing interests

The research staff declare that they have no competing interests. The project is partly funded by Arla Foods Ingredients Group P/S, Viby J, Denmark. The company has no influence on data analysis or publication strategy. AS is employed as a Nutrition Research Scientist at Arla Foods Ingredients P/S. The other authors declare that they have no competing interests.

Ethics approval and consent to participate

The Danish Regional Ethical Committees of the Capital Region has approved the protocol (J-nr. H-4-2013-070). Upon inclusion, all subjects give their written informed consent to participate in accordance with the Declaration of Helsinki II.

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Correspondence to Lars Holm.

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Bechshøft, R.L., Reitelseder, S., Højfeldt, G. et al. Counteracting Age-related Loss of Skeletal Muscle Mass: a clinical and ethnological trial on the role of protein supplementation and training load (CALM Intervention Study): study protocol for a randomized controlled trial. Trials 17, 397 (2016). https://0-doi-org.brum.beds.ac.uk/10.1186/s13063-016-1512-0

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