Construction of the Fingerprints of Ginseng Stem and Leaf Saponin Reference Substances and Spiked Plasma Sample by LC-ESI/MS and Its Application to Analyzing the Compounds Absorbed into Blood after Oral Administration of Ginseng Stem and Leaf Saponin in Rat

Bei Yan; Guangji Wang; Jiye A; Lin Xie; Haiping Hao; Yan Liang; Jianguo Sun; Xiaoyu Li; Yuanting Zheng

doi:10.1248/bpb.30.1657

Regular Articles

Construction of the Fingerprints of Ginseng Stem and Leaf Saponin Reference Substances and Spiked Plasma Sample by LC-ESI/MS and Its Application to Analyzing the Compounds Absorbed into Blood after Oral Administration of Ginseng Stem and Leaf Saponin in Rat

Bei Yan, Guangji Wang, Jiye A, Lin Xie, Haiping Hao, Yan Liang, Jianguo Sun, Xiaoyu Li, Yuanting Zheng

Author information

Bei Yan
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University
Guangji Wang
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University
Jiye A
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University
Lin Xie
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University
Haiping Hao
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University
Yan Liang
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University
Jianguo Sun
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University
Xiaoyu Li
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University
Yuanting Zheng
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University

Keywords: ginseng saponin, fingerprint, rat plasma, LC-ESI/MS

JOURNAL FREE ACCESS

2007 Volume 30 Issue 9 Pages 1657-1662

DOI https://doi.org/10.1248/bpb.30.1657

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Abstract

Develop a simple and reliable assay method to detect ginseng stem and leaf saponins (GSLS) in methanol and rat plasma by liquid chromatography-electrospray ionization mass spectrometry in scan mode, and construct the fingerprints of GSLS reference substances and plasma samples. In order to screen the active constituents of GSLS, analysis and comparison were carried out between the LC/ESI-MS profiles of blank rat plasma and rat plasma samples obtained after oral administration of GSLS. Thirty-one compounds were detected and 10 of them were identified in the fingerprints of reference substances and spiked plasma sample. Furthermore, 12 compounds (C7, C8, C14, C15, C18, Re, C24, Rb₁, Rc, Rb₂, Rb₃, Rd) were absorbed easily and some new compounds were generated after oral administration of GSLS, which might be the metabolites of GSLS. These absorbed components and new compounds may be the main bioactive components of GSLS.

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