Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
Purification of Calcium Binding Substance from Soluble Fraction of Normal Rat Liver
MASAYOSHI YAMAGUCHITAKEO YAMAMOTO
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Keywords: calcium metabolism
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1978 Volume 26 Issue 6 Pages 1915-1918

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Abstract

A calcium binding substance has been isolated from the soluble fraction of normal rat liver. The calcium binding substance was purified by means of gel filtration on Sephadex G-75 and G-50 followed by ion-exchange chromatography on diethylaminoethyl-cellulose. By these methods, the calcium binding activity from the soluble fraction has been purified several 100-fold and the homogeneity of the calcium binding substance has been established by polyacrylamide gel electrophoresis.

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© The Pharmaceutical Society of Japan
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