1978 Volume 26 Issue 6 Pages 1915-1918
A calcium binding substance has been isolated from the soluble fraction of normal rat liver. The calcium binding substance was purified by means of gel filtration on Sephadex G-75 and G-50 followed by ion-exchange chromatography on diethylaminoethyl-cellulose. By these methods, the calcium binding activity from the soluble fraction has been purified several 100-fold and the homogeneity of the calcium binding substance has been established by polyacrylamide gel electrophoresis.