Antioxidant Effects of Aqueous Extracts from Dried Calyx of Hibiscus sabdariffa L<small>INN</small>. (Roselle) in Vitro Using Rat Low-Density Lipoprotein (LDL)

Vilasinee Hirunpanich; Anocha Utaipat; Noppawan Phumala Morales; Nuntavan Bunyapraphatsara; Hitoshi Sato; Angkana Herunsalee; Chuthamanee Suthisisang

doi:10.1248/bpb.28.481

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Antioxidant Effects of Aqueous Extracts from Dried Calyx of Hibiscus sabdariffa LINN. (Roselle) in Vitro Using Rat Low-Density Lipoprotein (LDL)

Vilasinee Hirunpanich, Anocha Utaipat, Noppawan Phumala Morales, Nuntavan Bunyapraphatsara, Hitoshi Sato, Angkana Herunsalee, Chuthamanee Suthisisang

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Keywords: Hibiscus sabdariffa, roselle, low-density lipoprotein (LDL) oxidation, antioxidant

JOURNAL FREE ACCESS

2005 Volume 28 Issue 3 Pages 481-484

DOI https://doi.org/10.1248/bpb.28.481

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Abstract

The present study quantitatively investigated the antioxidant effects of the aqueous extracts from dried calyx of Hibiscus sabdariffa LINN. (roselle) in vitro using rat low-density lipoprotein (LDL). Formations of the conjugated dienes and thiobarbituric acid reactive substances (TBARs) were monitored as markers of the early and later stages of the oxidation of LDL, respectively. Thus, we demonstrated that the dried calyx extracts of roselle exhibits strong antioxidant activity in Cu²⁺-mediated oxidation of LDL (p<0.05) in vitro. The inhibitory effect of the extracts on LDL oxidation was dose-dependent at concentrations ranging from 0.1 to 5 mg/ml. Moreover, 5 mg/ml of roselle inhibited TBARs-formation with greater potency than 100 μM of vitamin E. In conclusion, this study provides a quantitative insight into the potent antioxidant effect of roselle in vitro.

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