Five hydroxylated sterols, stigmastane-3β, 6α-diol, poriferastane-3β, 6α-diol, stigmast-5-ene-3β, 4β-diol, poriferast-5-ene-3β, 4β-diol, and poriferasta-5, 25-diene-3β, 4β-diol, the latter four of which are new naturally occurring compounds, were isolated from the unsaponifiable lipid of the seed extract of Trichosanthes kirilowii MAXIM. The structures were determined by spectral and chemical methods.