Serum Level of Fibrinogen Aα Chain Fragment Increases in Chronic Thromboembolic Pulmonary Hypertension

Toshiaki Yano; Kazuyuki Sogawa; Hiroshi Umemura; Seiichiro Sakao; Yasunori Kasahara; Nobuhiro Tanabe; Yoshio Kodera; Yuichi Takiguchi; Koichiro Tatsumi; Fumio Nomura

doi:10.1253/circj.CJ-11-0045

Pulmonary Circulation

Serum Level of Fibrinogen Aα Chain Fragment Increases in Chronic Thromboembolic Pulmonary Hypertension

Toshiaki Yano, Kazuyuki Sogawa, Hiroshi Umemura, Seiichiro Sakao, Yasunori Kasahara, Nobuhiro Tanabe, Yoshio Kodera, Yuichi Takiguchi, Koichiro Tatsumi, Fumio Nomura

Author information

Toshiaki Yano
Department of Respirology, Graduate School of Medicine, Chiba University
Kazuyuki Sogawa
Clinical Proteomics Center, Chiba University Hospital
Hiroshi Umemura
Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University
Seiichiro Sakao
Department of Respirology, Graduate School of Medicine, Chiba University
Yasunori Kasahara
Department of Respirology, Graduate School of Medicine, Chiba University
Nobuhiro Tanabe
Department of Respirology, Graduate School of Medicine, Chiba University
Yoshio Kodera
Clinical Proteomics Center, Chiba University Hospital
Department of Physics, School of Science, Kitasato University
Yuichi Takiguchi
Department of Respirology, Graduate School of Medicine, Chiba University
Koichiro Tatsumi
Department of Respirology, Graduate School of Medicine, Chiba University
Fumio Nomura
Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University
Clinical Proteomics Center, Chiba University Hospital

Keywords: Biomarker, Chronic thromboembolic pulmonary hypertension, Fibrinogen Aα chain, Matrix-assisted laser desorption/ionization, Proteomics

JOURNAL FREE ACCESS

2011 Volume 75 Issue 11 Pages 2675-2682

DOI https://doi.org/10.1253/circj.CJ-11-0045

View "Advance Publication" version (September 2, 2011).

Details

Abstract

Background: The cause of chronic thromboembolic pulmonary hypertension is unknown and there is no specific circulating biomarker for its detection. The aim of the present study was to use proteomic analysis to detect serum biomarkers by evaluating the serum profiles of low-molecular-weight peptides using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry in patients with chronic thromboembolic pulmonary hypertension.Methods and Results: Serum low-molecular-weight peptide profiling using the spectrophotometric technique was studied retrospectively in patients with chronic thromboembolic pulmonary hypertension and in controls matched for sex and age. The serum level of a 2989-Da peptide in the sera of patients was significantly higher compared to that of controls. Tandem mass spectrometry indicated that the peptide was a fragment of fibrinogen Aα chain (KMADEAGSEADHEGTHSTKRGHAKSRPV). The serum level of fibrinogen Aα chain fragment, measured using a heavy isotope internal standard, tended toward negative correlation with plasmin-α2-plasmin inhibitor complex (P=0.073) and had a positive correlation with thrombin-anti-thrombin complex (P=0.031).Conclusions: This fragment may be a potential diagnostic biomarker for chronic thromboembolic pulmonary hyper-tension. (Circ J 2011; 75: 2675-2682)

Corresponding author

Register with J-STAGE for free!