Ethyl Acetate Soluble Fraction of Cnidium officinale <sc><font size=-2>MAKINO</font></sc> Inhibits Neuronal Cell Death by Reduction of Excessive Nitric Oxide Production in Lipopolysaccharide-Treated Rat Hippocampal Slice Cultures and Microglia Cells

Jeong Min Kim; Dongwook Son; Pyeongjae Lee; Kang Jin Lee; Hocheol Kim; Sun Yeou Kim

doi:10.1254/jphs.92.74

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Ethyl Acetate Soluble Fraction of Cnidium officinale MAKINO Inhibits Neuronal Cell Death by Reduction of Excessive Nitric Oxide Production in Lipopolysaccharide-Treated Rat Hippocampal Slice Cultures and Microglia Cells

Jeong Min Kim, Dongwook Son, Pyeongjae Lee, Kang Jin Lee, Hocheol Kim, Sun Yeou Kim

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Keywords: Cnidium officinale MAKINO, nitric oxide, hippocampal slice

JOURNAL FREE ACCESS

2003 Volume 92 Issue 1 Pages 74-78

DOI https://doi.org/10.1254/jphs.92.74

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Abstract

In the present work, we found that the ethyl acetate-soluble fraction of Cnidium officinale MAKINO (COEA) decreased nitric oxide (NO) production in the lipopolysaccharide (LPS)-stimulated BV-2 and primary microglia and suppressed expression of inducible nitric oxide synthase (iNOS) in BV-2 cells with the same pattern of NO production. In addition, we showed that excessive NO production played an important role in neuronal cell death in LPS-treated rat hippocampal slice cultures. Our data suggest that the COEA inhibits neuronal cell death by reduction of excessive NO production in LPS-treated rat hippocampal slice cultures. The ethyl acetate-soluble fraction of C. officinale reduced propidium iodide uptake and NO production in cultured media at the same time.

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